Mouse Galectin-9 Antibody Summary
Accession # O08573-2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Galectin‑9 in Mouse Colon. Galectin‑9 was detected in perfusion fixed frozen sections of mouse colon using Rat Anti-Mouse Galectin‑9 Monoclonal Antibody (Catalog # MAB3535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm of epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Galectin-9 in Mouse Thymocytes by Flow Cytometry.
Mouse thymocytes were stained with Rat Anti-Mouse Galectin‑9 Monoclonal Antibody (Catalog # MAB3535, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse Galectin-9 ( beta -galactoside-binding lectin 9) is a 36-39 kDa, secreted, S-type lectin. It is 353 amino acids in length and contains no identifiable signal sequence. There are two distinct carbohydrate-binding regions (aa 81-87 and 285-291) that are joined by a linker region (aa 148-205). At least one alternate splice form exists that shows a 31 aa insertion between aa 147-148. The short form is expressed on fibroblasts, hepatocytes, endothelial cells and astrocytes. The long form is expressed on intestinal epithelium. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 is 69% and 85% aa identical to human and rat Galectin-9, respectively.
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Cell and Tissue Staining Kits
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