Mouse Galectin-9 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Galectin-9 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Galectin-9 or recombinant mouse Galectin-4 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 766428
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Galectin-9 (short isoform)
    Ala2-Thr322
    Accession # O08573-2
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Galectin‑9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse Galectin‑9 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC3535G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Galectin-9
Mouse Galectin-9 ( beta -Galactoside-binding Lectin 9) is a 36-39 kDa, secreted, S-type lectin. It is 353 amino acids (aa) in length and contains no identifiable signal sequence. There are two distinct carbohydrate-binding regions (aa 81-87 and 285-291) that are joined by a linker region (aa 148-205). At least one alternate splice form exists that shows a 31 aa insertion between aa 147-148. The short form is expressed on fibroblasts, hepatocytes, endothelial cells and astrocytes. The long form is expressed on intestinal epithelium. Galectin-9 binds TIM-3 on Th1 cells, inducing apoptosis. Mouse Galectin-9 is 69% and 85% aa identical to human and rat Galectin-9, respectively.
  • Entrez Gene IDs:
    3965 (Human); 16859 (Mouse)
  • Alternate Names:
    Ecalectin; GAL9; gal-9; galectin 9; Galectin9; Galectin-9; HUAT; lectin, galactoside-binding, soluble, 9; LGALS9; LGALS9A; MGC117375; MGC125973; MGC125974; Tumor antigen HOM-HD-21; urate transporter/channel protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC013G
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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