Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently upregulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25+ regulatory CD4+ T cells (Treg). GITR is also expressed on naïve CD4+ CD25- T cells, where its expression is upregulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides costimulatory signals for activated CD4+ CD25- T cells to enhance cell proliferation and augment cytokine production. On CD4+ CD25+ Treg cells, GITRL also provides costimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important costimulatory signals that play important roles in modulating diverse T cell functions (1-4).
Mouse GITR Ligand/TNFSF18 Alexa Fluor® 405‑conjugated Antibody
R&D Systems | Catalog # FAB21773V
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Thr47-Ser173
Accession # Q7TS55
Specificity
Clonality
Host
Isotype
Applications for Mouse GITR Ligand/TNFSF18 Alexa Fluor® 405‑conjugated Antibody
Flow Cytometry
Sample: NS0 Cell Line Transfected with Mouse GITR Ligand/TNFSF18
Spectra Viewer
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Formulation, Preparation, and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Calculators
Background: GITR Ligand/TNFSF18
References
- Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
- Ji, H. et al. (2004) J. Immunology 172:5823.
- Kanamaru, F. et al. (2004) J. Immunology 172:7306.
- Ronchetti, S. et al. (2004) Eur. J. Immunology 34:613.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional GITR Ligand/TNFSF18 Products
Product Documents for Mouse GITR Ligand/TNFSF18 Alexa Fluor® 405‑conjugated Antibody
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Product Specific Notices for Mouse GITR Ligand/TNFSF18 Alexa Fluor® 405‑conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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