Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 698423
View all GM-CSF R alpha Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GM-CSF R alpha
Leu30-Pro327
Accession # Q00941

Specificity

Detects mouse GM-CSF R alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) GM-CSF R alpha or rhGM‑CSF R beta is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse GM‑CSF R alpha PE‑conjugated Antibody

Detection of GM-CSF Ra antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry.

Detection of GM-CSF R alpha in J774A.1 Mouse Cell Line by Flow Cytometry.

J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM-CSF Ra PE-conjugated Monoclonal Antibody (Catalog # FAB6130P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse GM‑CSF R alpha PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line

Reviewed Applications

Read 1 review rated 3 using FAB6130P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: GM-CSF R alpha

Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature mouse GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the ECD, mouse GM-CSF R alpha shares approximately 33% and 58% aa sequence identity with human and rat GM-CSF R alpha, respectively. Soluble forms of the human receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non‑hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).

References

  1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  2. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
  3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
  4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
  5. Park, L.S. et al. (1992) Proc. Natl. Acad. Sci. 89:4295.
  6. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
  7. Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
  8. Chiba, S. et al. (1990) Cell Regul. 1:327.
  9. Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
  10. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
  11. Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.

Long Name

Granulocyte Macrophage Growth Factor Receptor alpha

Alternate Names

CD116, CSF2R, CSF2RA, CSF2RY, GM-CSFRa, GMCSFR alpha

Entrez Gene IDs

1438 (Human); 12982 (Mouse); 102139775 (Cynomolgus Monkey)

Gene Symbol

CSF2RA

UniProt

Q00941

Additional GM-CSF R alpha Products

Product Documents for Mouse GM‑CSF R alpha PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Mouse GM‑CSF R alpha PE‑conjugated Antibody

For research use only

Citations for Mouse GM‑CSF R alpha PE‑conjugated Antibody

Customer Reviews for Mouse GM‑CSF R alpha PE‑conjugated Antibody (1)

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  • Mouse GM-CSF R alpha PE-conjugated Antibody
    Name: Scott Fites
    Application: Flow Cytometry
    Sample Tested: Neutrophils
    Species: Mouse
    Verified Customer | Posted 07/21/2018
    Neutrophils were differentiated in vitro from granulocyte/macrophage progenitors. Cells were stained for neutrophil surface markers (CD11b, Gr-1) and populations show gated neutrophils. Anti-GM-CSFR alpha antibody was titrated in the surface stain or after surface staining with intracellular staining after cells were fixed and permeablized. Like for many surface receptors, the antibody did not stain well when use to stain the surface of cells; however, a positive stain was seen with intracellular staining. There isn't a huge difference between positive staining and the FMO control minimizing the applications for this antibody for flow cytometry.
    Mouse GM‑CSF R alpha PE‑conjugated Antibody FAB6130P

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