Mouse GM-CSF R alpha Antibody Summary
rhGM-CSF R beta is observed.
Accession # Q00941
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of GM-CSF R alpha in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM-CSF Ra Monoclonal Antibody (Catalog # MAB6130, filled histo-gram) or isotype control antibody (Catalog # MAB006, open histo-gram), followed by Allophy-cocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Cell Proliferation Induced by GM‑CSF and Neutralization by Mouse GM‑CSF R alpha Antibody. Recombinant Mouse GM-CSF (Catalog # 415-ML) stimulates proliferation in the DA3 mouse myeloma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse GM-CSF (0.1 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse GM-CSF Ra Monoclonal Anti-body (Catalog # MAB6130). The ND50 is typically 0.02-0.12 µg/mL.
GM‑CSF R alpha in J774A.1 Mouse Cell Line. GM-CSF Ra was detected in immersion fixed J774A.1 mouse reticulum cell sarcoma macrophage cell line using Rat Anti-Mouse GM-CSF Ra Mono-clonal Antibody (Catalog # MAB6130) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM-CSF R alpha
Granulocyte macrophage colony stimulating factor receptor alpha (GM-CSF R alpha ), also known as CD116, is a component of the receptor complex that mediates cellular responses to GM-CSF. GM-CSF promotes the differentiation and mobilization of granulocyte-macrophage, erythroid, megakaryocyte, and eosinophil progenitors. It enhances the activation of myeloid cell effector functions and plays a role in the development of Th1 biased immune responses, allergic inflammation, and autoimmunity (1-4). Mature mouse GM-CSF R alpha is an 80 kDa type I transmembrane glycoprotein that consists of a 298 amino acid (aa) extracellular domain (ECD) with two fibronectin type III domains and a juxtamembrane WSXWS motif, a 21 aa transmembrane segment, and a 40 aa cytoplasmic domain (5). Within the ECD, mouse GM-CSF R alpha shares approximately 33% and 58% aa sequence identity with human and rat GM-CSF R alpha, respectively. Soluble forms of the human receptor retain the ability to bind GM-CSF (6, 7). GM-CSF R alpha is expressed on hematopoietic stem cells, progenitor and differentiated cells in the myeloid lineage, vascular endothelial cells, placenta, and non‑hematopoietic solid tumor cells (8). GM-CSF R alpha associates with the common beta chain/CD131 ( beta c), a 135 kDa transmembrane protein that is also the signal transducing component of the receptors for IL-3 and IL-5 (9, 10). Association with beta c converts GM-CSF R alpha from a low affinity to a high affinity receptor for GM-CSF (9-11). The shared usage of beta c underlies the synergism between GM-CSF, IL-3, and IL-5 in their effects on myeloid cell differentiation and activation (1, 2).
- Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
- Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
- Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
- Cao, Y. (2007) J. Clin. Invest. 117:2362.
- Park, L.S. et al. (1992) Proc. Natl. Acad. Sci. 89:4295.
- Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
- Raines, M.A. et al. (1991) Proc. Natl. Acad. Sci. 88:8203.
- Chiba, S. et al. (1990) Cell Regul. 1:327.
- Kitamura, T. et al. (1991) Proc. Natl. Acad. Sci. 88:5082.
- Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
- Hoang, T. et al. (1993) J. Biol. Chem. 268:11881.
Citations for Mouse GM-CSF R alpha Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
Filter your results:
STAT3 inhibition induces Bax-dependent apoptosis in liver tumor myeloid-derived suppressor cells
Authors: P Guha, J Gardell, J Darpolor, M Cunetta, M Lima, G Miller, NJ Espat, RP Junghans, SC Katz
Sample Types: Tissue Homogenates
Applications: Western Blot
Essential Role of mTORC1 in Self-Renewal of Murine Alveolar Macrophages
Authors: Xiao-Ping Zhong
J. Immunol., 2016;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
Coincidental null mutation of Csf2r? in a colony of PI3K?-/- mice causes alveolar macrophage deficiency and fatal respiratory viral infection
J Leukoc Biol, 2016;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
Pleural innate response activator B cells protect against pneumonia via a GM-CSF-IgM axis.
Authors: Weber G, Chousterman B, Hilgendorf I, Robbins C, Theurl I, Gerhardt L, Iwamoto Y, Quach T, Ali M, Chen J, Rothstein T, Nahrendorf M, Weissleder R, Swirski F
J Exp Med, 2014;211(6):1243-56.
Sample Types: In Vivo
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Mouse GM-CSF R alpha Antibody
There are currently no reviews for this product. Be the first to review Mouse GM-CSF R alpha Antibody and earn rewards!
Have you used Mouse GM-CSF R alpha Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image