Detection of GPVI in Mouse Platelets by Flow Cytometry.
Mouse platelets were stained with Rat Anti-Mouse GPVI PE‑conjugated Monoclonal Antibody (Catalog # FAB6758P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
GPVI (Platelet Glycoprotein VI), also known as Glycoprotein 5, is a member of the Ig superfamily. It is found on platelets and megakaryocytes, and serves as the main collagen receptor on platelets. Following exposure to subendothelial connective tissue, GPVI binds to a Gly-Pro-(hydroxy)Pro motif on collagen and generates a noncovalent membrane signaling complex with FcR gamma -chain. This interaction is stabilized by Integrin alpha 2 beta 1, followed by activation of PLC gamma 2 with clot initiation. Mature mouse GPVI is a 292 amino acid (aa) type I transmembrane protein. It possesses a 244 aa extracellular region (aa 22-265) that contains two C2-type Ig-like domains (aa 27-197)and two potential glycosylation sites, plus a 37 aa cytoplasmic tail (aa 287-313). There is one potential splice form that shows a deletion of aa 224-240. Over aa 24-265, mouse GPVI shares 70% and 86% aa identity with human and rat GPVI, respectively.
Glycoprotein VI [Platelet]
Entrez Gene IDs:
51206 (Human); 243816 (Mouse)
Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVI; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI
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