Mouse GPVI PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB6758P
Detection of GPVI in Mouse Platelets by Flow Cytometry.
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Product Details
Citations (1)
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Mouse GPVI PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GPVI in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human GPVI is observed.
Source
Monoclonal Rat IgG1 Clone # 784808
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GPVI
Gly24-Lys265
Accession # P0C191
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GPVI antibody in Mouse Platelets antibody by Flow Cytometry. View Larger

Detection of GPVI in Mouse Platelets by Flow Cytometry. Mouse platelets were stained with Rat Anti-Mouse GPVI PE-conjugated Monoclonal Antibody (Catalog # FAB6758P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: GPVI

GPVI (Platelet Glycoprotein VI), also known as Glycoprotein 5, is a member of the Ig superfamily. It is found on platelets and megakaryocytes, and serves as the main collagen receptor on platelets. Following exposure to subendothelial connective tissue, GPVI binds to a Gly-Pro-(hydroxy)Pro motif on collagen and generates a noncovalent membrane signaling complex with FcR gamma -chain. This interaction is stabilized by Integrin alpha 2 beta 1, followed by activation of PLC gamma 2 with clot initiation. Mature mouse GPVI is a 292 amino acid (aa) type I transmembrane protein. It possesses a 244 aa extracellular region (aa 22-265) that contains two C2-type Ig-like domains (aa 27-197)and two potential glycosylation sites, plus a 37 aa cytoplasmic tail (aa 287-313). There is one potential splice form that shows a deletion of aa 224-240. Over aa 24-265, mouse GPVI shares 70% and 86% aa identity with human and rat GPVI, respectively.

Long Name
Glycoprotein VI [Platelet]
Entrez Gene IDs
51206 (Human); 243816 (Mouse)
Alternate Names
Glycoprotein 6; glycoprotein VI (platelet); GP6; GPIV; GPVI; GPVIplatelet collagen receptor; MGC138168; platelet glycoprotein VI

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Citation for Mouse GPVI PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Platelets from mice lacking the aryl hydrocarbon receptor exhibit defective collagen-dependent signaling.
    Authors: Lindsey S, Jiang J, Woulfe D, Papoutsakis E
    J Thromb Haemost, 2014-01-01;12(3):383-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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