Mouse H60 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1155P
Detection of H60 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (6)
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Mouse H60 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse H60 in ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 205326
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with mouse H60 and mouse myeloma cell line NS0-derived recombinant mouse H60
Asp30-Gln212
Accession # Q3TDZ7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of H60 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of H60 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse H60 PE-conjugated Monoclonal Antibody (Catalog # FAB1155P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: H60

H60 was originally described as an immunodominant histocompatibility antigen that is expressed in BALB mice but not in B6 mice. More recently it was reported to function as a ligand for mouse NKG2D, an activating receptor found on NK cells, on some T cell subsets, and on stimulated macrophages. H60 shares approximately 25 percent amino acid identity with the Rae-1 family, a small group of proteins that also function as ligands for mouse NKG2D. H60 and the Rae-1 proteins are distantly related to MHC class I proteins, but they possesses only the alpha 1 and alpha 2 Ig-like domains, and have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. Unlike the GPI-linked Rae-1 proteins, H60 appears to be anchored to the membrane via a hydrophobic transmembrane segment. H60 transcripts were found in embryonic tissue, in spleen, and in some transformed cell lines. Transcripts were also observed in mouse skin cells after exposure to carcinogens. Binding of H60 to NKG2D results in the activation of cytolytic activity and/or cytokine production by the NKG2D-expressing effector cells. Ectopic expression of H60 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1-6).

References
  1. Malarkannan, S. et al. (1998) J. Immunol. 161:3501.
  2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
  3. Cerwenka, A. et al. (2000) Immunity 12:721.
  4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  5. Diefenbach, A. et al. (2001) Nature 413:165.
  6. NKG2D and its Ligands, www.RnDSystems.com.
Long Name
Histocompatibility 60a
Entrez Gene IDs
15101 (Mouse)
Alternate Names
H60; H60a

Product Datasheets

Citations for Mouse H60 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Immunotherapy of Dual-Function Vector with Both Immunostimulatory and B-Cell Lymphoma 2 (Bcl-2)-Silencing Effects on Gastric Carcinoma
    Authors: L Ma, M Han, Z Keyoumu, H Wang, S Keyoumu
    Med. Sci. Monit., 2017;23(0):1980-1991.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Neural stem cells sustain natural killer cells that dictate recovery from brain inflammation.
    Authors: Liu Q, Sanai N, Jin W, La Cava A, Van Kaer L, Shi F
    Nat Neurosci, 2016;19(2):243-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. In vivo eradication of MLL/ENL leukemia cells by NK cells in the absence of adaptive immunity.
    Authors: Nakata J, Nakano K, Okumura A, Mizutani Y, Kinoshita H, Iwai M, Hasegawa K, Morimoto S, Fujiki F, Tatsumi N, Nakajima H, Nakae Y, Nishida S, Tsuboi A, Oji Y, Oka Y, Sugiyama H, Kumanogoh A, Hosen N
    Leukemia, 2014;28(6):1316-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.
    J. Immunol., 2012;189(3):1162-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Small intestinal intraepithelial lymphocytes expressing CD8 and T cell receptor gammadelta are involved in bacterial clearance during Salmonella enterica serovar Typhimurium infection.
    Authors: Li Z, Zhang C, Zhou Z, Zhang J, Zhang J, Tian Z
    Infect. Immun., 2012;80(2):565-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. NKG2D receptor signaling enhances cytolytic activity by virus-specific CD8+ T cells: evidence for a protective role in virus-induced encephalitis.
    Authors: Walsh KB, Lanier LL, Lane TE
    J. Virol., 2007;82(6):3031-44.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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