Mouse H60 Antibody

Catalog # Availability Size / Price Qty
MAB1155
MAB1155-SP
Detection of H60 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (5)
FAQs
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Mouse H60 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse H60 in ELISAs and Western blots.
Source
Monoclonal Rat IgG2A Clone # 205326
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with mouse H60 and mouse myeloma cell line NS0-derived recombinant mouse H60
Asp30-Gln212
Accession # Q3TDZ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse H60 Fc Chimera (Catalog # 1155-H6) under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.1-0.3 µg/mL of this antibody will block 50% of the binding of 125 ng/mL of biotinylated Recombinant Mouse NKG-2D Fc Chimera to immobilized Recombinant Mouse H60 Fc Chimera (Catalog # 1155-H6) coated at 2 µg/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Mouse H60 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse H60 Biotinylated Antibody (Catalog # BAF1155)

Standard: Recombinant Mouse H60 Fc Chimera Protein, CF (Catalog # 1155-H6)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of H60 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of H60 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse H60 Monoclonal Antibody (Catalog # MAB1155, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by anti-Rat IgG PE-conjugated Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: H60

H60 was originally described as an immunodominant histocompatibility antigen that is expressed in BALB mice but not in B6 mice. More recently it was reported to function as a ligand for mouse NKG2D, an activating receptor found on NK cells, on some T cell subsets, and on stimulated macrophages. H60 shares approximately 25 percent amino acid identity with the Rae-1 family, a small group of proteins that also function as ligands for mouse NKG2D. H60 and the Rae-1 proteins are distantly related to MHC class I proteins, but they possesses only the alpha 1 and alpha 2 Ig-like domains, and have no capacity to bind peptide or interact with beta 2‑microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. Unlike the GPI-linked Rae-1 proteins, H60 appears to be anchored to the membrane via a hydrophobic transmembrane segment. H60 transcripts were found in embryonic tissue, in spleen, and in some transformed cell lines. Transcripts were also observed in mouse skin cells after exposure to carcinogens. Binding of H60 to NKG2D results in the activation of cytolytic activity and/or cytokine production by the NKG2D-expressing effector cells. Ectopic expression of H60 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1‑6).

References
  1. Malarkannan, S. et al. (1998) J. Immunol. 161:3501.
  2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.
  3. Cerwenka, A. et al. (2000) Immunity 12:721.
  4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.
  5. Diefenbach, A. et al. (2001) Nature 413:165.
  6. NKG2D and its Ligands, www.RnDSystems.com.
Long Name
Histocompatibility 60a
Entrez Gene IDs
15101 (Mouse)
Alternate Names
H60; H60a

Product Datasheets

Citations for Mouse H60 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Visualizing the Rapid and Dynamic Elimination of Allogeneic T Cells in Secondary Lymphoid Organs
    Authors: Y Kanda, A Takeuchi, M Ozawa, Y Kurosawa, T Kawamura, D Bogdanova, H Iioka, E Kondo, Y Kitazawa, H Ueta, K Matsuno, T Kinashi, T Katakai
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. NKG2D-NKG2D Ligand Interaction Inhibits the Outgrowth of Naturally Arising Low-Grade B Cell Lymphoma In Vivo
    J Immunol, 2016;196(11):4805-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
    Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
    J Invest Dermatol, 2015;135(5):1311-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
    Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
    Blood, 2009;113(24):6120-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. A role for activation-induced cytidine deaminase in the host response against a transforming retrovirus.
    Authors: Gourzi P, Leonova T, Papavasiliou FN
    Immunity, 2006;24(6):779-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. Which isoform of H60 does Mouse H60 Antibody, Catalog # MAB1155, detect?

    • MAB1155 detects H60a. The immunogen sequence is Asp30-Gln212 from Accession # Q3TDZ7.

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