Mouse ICAM-1/CD54 PE-conjugated Antibody

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Detection of ICAM‑1/CD54 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (1)
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Mouse ICAM-1/CD54 PE-conjugated Antibody Summary

Species Reactivity
Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and recombinant mouse (rm) ICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, recombinant rat (rr) ICAM-1, rmMAdCAM-1, or rmVCAM-1.
Monoclonal Rat IgG2B Clone # 166623
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1
Accession # P13597
Supplied in a saline solution containing BSA and Sodium Azide.
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)


Recommended Concentration
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ICAM-1/CD54 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of ICAM‑1/CD54 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse ICAM-1/CD54 PE-conjugated Monoclonal Antibody (Catalog # FAB796P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: ICAM-1/CD54

ICAM-1 (InterCellular Adhesion Molecule-1), also known as CD54, is an 85-93 kDa member of the Immunoglobulin supergene family of molecules. Mouse ICAM-1 is a 510 amino acid (aa) type I transmembrane glycoprotein that contains five C2-type Ig-like domains in a 458 aa extracellular domain (ECD) (aa 28-485). It is expressed constitutively by, or inducibly on, a very wide variety of cells, including neurons, dendritic cells, fibroblasts, skeletal muscle myocytes, keratinocytes, eosinophils, neutrophils, hepatocytes, monocytes and vascular endothelial cells. On the cell surface, it exists primarily as an "intermediate" between a homodimeric and monomeric state. The homodimeric pairing does not seem to offer a distinct advantage with respect to ligand binding. On the other hand, the degree of glycosylation has a marked impact on ICAM-1 ligand binding, with under-glycosylation associated with low affinity ligand binding. Ligands for ICAM-1 include LFA-1 ( alpha L beta 2), Mac-1 ( alpha M beta 2), CD11c/CD18, fibrinogen, hyaluronan and select rhinoviruses. Notably, ICAM-1 is recognized to be a potential coreceptor for HGF, acting in concert with c-Met during ligand binding. ICAM-1 is best known for its role in white cell trafficking (leukocyte binding to endothelium), and is also reported to play a key role in the establishment of CD8+ T cell memory, serving as a link between mature dendritic cells and naïve CD8+ T cells. Over its ECD, mouse ICAM-1 shares 78% and 54% aa sequence identity with rat and human ICAM-1 ECD, respectively. There is one isoform variant for mouse ICAM-1 listed in SwissProt that shows a 28 aa substitution for aa 1-34.

Long Name
Intercellular Adhesion Molecule 1
Entrez Gene IDs
3383 (Human); 15894 (Mouse); 25464 (Rat)
Alternate Names
BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58

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Citation for Mouse ICAM-1/CD54 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ICAM-1-based rabies virus vaccine shows increased infection and activation of primary murine B cells in vitro and enhanced antibody titers in-vivo.
    Authors: Norton, James E, Lytle, Andrew G, Shen, Shixue, Tzvetkov, Evgeni P, Dorfmeier, Corin L, McGettigan, James P
    PLoS ONE, 2014-01-29;9(1):e87098.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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