Mouse ICAM‑1/CD54 Antibody

Name: Mouse ICAM‑1/CD54 Antibody
Brand: R&D Systems
SKU: MAB796
Price: 489 USD
Availability: InStock

R&D Systems | Catalog # MAB796

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Citations (9)

Product Documents

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Western Blot, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 166623

View all ICAM-1/CD54 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ICAM-1
Gln28-Asn485
Accession # P13597

Specificity

Detects mouse ICAM-1 in ELISAs and Western blots. In Western blots, this antibody shows weak cross-reactivity with recombinant human (rh) ICAM‑1 and rmICAM-2 and no cross-reactivity with rhCD31, rmDCC, rhICAM-2, rhICAM-3, rrICAM-1, rmMAdCAM-1, or rmVCAM-1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Applications for Mouse ICAM‑1/CD54 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse ICAM‑1/CD54 Fc Chimera (Catalog # 796-IC)

Mouse ICAM-1/CD54 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Mouse ICAM‑1/CD54 Biotinylated Antibody (Catalog # BAF796)
Standard: Recombinant Mouse ICAM-1/CD54 Fc Chimera Protein, CF (Catalog # 796-IC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Mouse and human ICAM-1 share approximately 54% amino acid sequence identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

References

Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
Siu, G. et al. (1989) J. Immunol. 143:3813.
Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.