Key Product Details

Species Reactivity

Mouse

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 996586
View all IFN-alpha 2/IFNA2 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2
Cys24-Glu190
Accession # P01573

Specificity

Detects mouse IFN-alpha 2/IFNA2 in direct ELISA. In direct ELISAs, no cross-reactivity with mouse IFN-alpha 1, 4, 9, 11, 12 and 15 was observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse IFN-alpha 2/IFNA2 Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with mouse anti-IFNA-2 Monoclonal Antibody (Catalog # MAB101491). This product is intended for assay development on various assay platforms requiring antibody pairs.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha 2/IFNA2

Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).

References

  1. Pestka, S. (2007) J Biol Chem. 282:20047.
  2. Paul, F. et al. (2015) Gene. 567(2):132.
  3. Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
  4. Pesch, V. et al. (2004). Journal of Virology, 78:8219.
  5. Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.
  6. Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.

Long Name

Interferon alpha 2

Alternate Names

Ifa2, IFNA2, IFNA2a, IFNA2b, IFNA2c, IFNAA, IFNalpha 2

Entrez Gene IDs

3440 (Human); 15965 (Mouse); 298213 (Rat)

Gene Symbol

IFNA2

UniProt

P01573

Additional IFN-alpha 2/IFNA2 Products

Product Documents for Mouse IFN-alpha 2/IFNA2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IFN-alpha 2/IFNA2 Antibody

For research use only

Related Research Areas

Interferons

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