Mouse IFN-beta Antibody

Catalog # Availability Size / Price Qty
32400-1
Product Details
Citations (4)
FAQs
Reviews

Mouse IFN-beta Antibody Summary

Species Reactivity
Mouse
Specificity
Mouse IFN-beta
Source
Polyclonal Rabbit Serum
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IFN-beta
Formulation
Neat serum diluted in phosphate buffered saline (PBS)
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of mouse interferon beta (Mu-IFN-beta ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using L929 cells and EMCV as the challenge virus. In this antiviral assay for interferon, about 2.5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Mu-IFN-beta provided by the National Institutes of Health [Gb02-902-511]. This material is prepared specifically for effective neutralization of Mu-IFN-beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, this product should be kept at -70˚C or below for retention of full activity.Thaw product vial by incubation in cold tap water until just thawed – the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

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Citations for Mouse IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. ZBP1 promotes inflammatory responses downstream of TLR3/TLR4 via timely delivery of RIPK1 to TRIF
    Authors: HI Muendlein, WM Connolly, Z Magri, D Jetton, I Smirnova, A Degterev, S Balachandr, A Poltorak
    Proceedings of the National Academy of Sciences of the United States of America, 2022-06-06;119(24):e2113872119.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  2. Constitutive Interferon Maintains GBP Expression Required for Release of Bacterial Components Upstream of Pyroptosis and Anti-DNA Responses
    Authors: BC Liu, J Sarhan, A Panda, HI Muendlein, V Ilyukha, J Coers, M Yamamoto, RR Isberg, A Poltorak
    Cell Rep, 2018-07-03;24(1):155-168.e5.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  3. Evaluating vaccinia virus cytokine co-expression in TLR GKO mice.
    Authors: Sutherland DB, Ranasinghe C, Regner M, Phipps S, Matthaei KI, Day SL, Ramshaw IA
    Immunol. Cell Biol., 2010-12-21;89(0):706-15.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  4. A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression.
    Authors: Joshi VD, Kalvakolanu DV, Chen W, Zhang L, Kang TJ, Thomas KE, Vogel SN, Cross AS
    J. Interferon Cytokine Res., 2006-10-01;26(10):739-47.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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