Mouse IFN-gamma R2 Antibody (MOB47)

R&D Systems | Catalog # MAB773

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Rat

Applications

Validated:

Western Blot, Neutralization, Flow Cytometry, CyTOF-ready

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Hamster IgG Clone # MOB47
View all IFN-gamma R2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse IFN-gamma R2
Extracellular domain

Specificity

Detects mouse IFN‑ gamma R2 extracellular domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IFN‑ gamma  R2, recombinant mouse IFN-gamma R1, rhIFN-gamma R1, or rhIL-10 R beta is observed.

Clonality

Monoclonal

Host

Hamster

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse IFN-gamma R2 Antibody (MOB47)

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R2 Antibody.

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R2 Antibody.

Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse IFN-gamma R2 Monoclonal Antibody (Catalog # MAB773). The ND50 is typically 0.2-1 µg/mL.

Applications for Mouse IFN-gamma R2 Antibody (MOB47)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: This antibody has been shown to recognize mouse IFN-gamma R2 expressed on mouse T cells (Bach, E. et al., 1995, Science 270:1215). This application was not tested by R&D Systems.

Western Blot

1 µg/mL
Sample: Recombinant Mouse IFN-gamma  R2

Neutralization

Measured by its ability to neutralize IFN‑ gamma  R2-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Mouse IFN‑ gamma.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma R2

Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma  R2 is required for signal transduction but does not bind IFN-gamma by itself.

Long Name

Interferon gamma Receptor 2

Alternate Names

AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1

Entrez Gene IDs

3460 (Human); 15980 (Mouse)

Gene Symbol

IFNGR2

Additional IFN-gamma R2 Products

Product Documents for Mouse IFN-gamma R2 Antibody (MOB47)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IFN-gamma R2 Antibody (MOB47)

For research use only

Citations for Mouse IFN-gamma R2 Antibody (MOB47)

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