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Mouse IFN-gamma  R2 Antibody

Catalog #: MAB773
1 Citation Datasheet / COA / SDS
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MAB773
MAB773-SP

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IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R2 Antibody.
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Mouse IFN-gamma  R2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IFN‑ gamma R2 extracellular domain in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IFN‑ gamma  R2, recombinant mouse IFN-gamma R1, rhIFN-gamma R1, or rhIL-10 R beta is observed.
Source
Monoclonal Hamster IgG Clone # MOB47
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Recombinant mouse IFN-gamma R2
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IFN-gamma  R2
Flow Cytometry
2.5 µg/106 cells
This antibody has been shown to recognize mouse IFN-gamma R2 expressed on mouse T cells (Bach, E. et al., 1995, Science 270:1215). This application was not tested by R&D Systems.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IFN‑ gamma  R2-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Vogel, S. and M. Hogan (1995) in Current Protocols in Immunology. Ciocio, R. (ed); John Wiley & Sons, Inc. p. 6. 9. 1. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Mouse IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R2 Antibody. View Larger

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R2 Antibody. Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse IFN-gamma R2 Monoclonal Antibody (Catalog # MAB773). The ND50 is typically 0.2-1 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R2

Interferon gamma Receptor 2 (IFN-gamma R2) is a type II transmembrane protein that associates with IFN-gamma R1 to form a high affinity receptor complex for IFN-gamma. IFN‑ gamma  R2 is required for signal transduction but does not bind IFN-gamma by itself.

Long Name
Interferon gamma Receptor 2
Entrez Gene IDs
3460 (Human); 15980 (Mouse)
Alternate Names
AF-1; AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1; IFGR2; IFN-gamma R2; IFN-gamma receptor 2; IFNgammaR2; IFN-gamma-R2; IFNGR2; IFN-gR2; IFNGT1; interferon gamma receptor 2 (interferon gamma transducer 1); interferon gamma receptor 2; interferon gamma receptor accessory factor-1; interferon gamma receptor beta chain; Interferon gamma transducer 1

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Citation for Mouse IFN-gamma  R2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Fibroblast growth factor inhibits interferon gamma-STAT1 and interleukin 6-STAT3 signaling in chondrocytes.
    Authors: Krejci P, Prochazkova J, Bryja V, Jelinkova P, Pejchalova K, Kozubik A, Thompson LM, Wilcox WR
    Cell. Signal., 2008-10-12;21(1):151-60.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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