Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2213C
View all IgG1 Primary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

Detects mouse IgG1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with mouse IgG2B, mouse IgG2A, and mouse IgG3 was observed.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse IgG1 PE‑conjugated Antibody

Detection of Mouse IgG1 Primary Antibody by Anti-Mouse IgG1-specific Secondary Antibody.

Detection of Mouse IgG1 Primary Antibody by Anti-Mouse IgG1-specific Secondary Antibody.

Human PBMC lymphocytes were stained with Mouse anti-HumanCD3 epsilon Monoclonal Antibody (Catalog # MAB100, Mouse IgG1 isotype, filled histogram), or Mouse anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B isotype, open histogram), followed by Rabbit anti-Mouse IgG1-specific PE-conjugated secondary antibody (Catalog #FAB9627P). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IgG1 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Cells stained with Mouse IgG1 Primary Antibody.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

Additional IgG1 Products

Product Documents for Mouse IgG1 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG1 PE‑conjugated Antibody

For research use only

Citations for Mouse IgG1 PE‑conjugated Antibody

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