Human CD3 epsilon Antibody

Clone UCHT1 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB100-SP
MAB100-100
MAB100-500
Detection of CD3 epsilon  in Human Lymphocytes by Flow Cytometry.
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Product Details
Procedure
Citations (46)
FAQs
Supplemental Products
Reviews (4)

Human CD3 epsilon Antibody Summary

Species Reactivity
Human
Specificity
Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
Source
Monoclonal Mouse IgG1 Clone # UCHT1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human thymocytes (1)
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation
McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York
 
CyTOF-reported
Behbehani, G.K. et al. (2015) Cancer Discov. 5: 988. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
T Cell Stimulation
This antibody can be used to activate T cells when immobilized at 1-10 μg/mL (100 μL/well).
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CD3 epsilon  in Human Lymphocytes by Flow Cytometry. View Larger

Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Immunocytochemistry CD3 epsilon  in Human PBMCs. View Larger

CD3 epsilon in Human PBMCs. CD3 epsilon was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

References
  1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
Entrez Gene IDs
916 (Human); 12501 (Mouse)
Alternate Names
CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

Product Datasheets

Citations for Human CD3 epsilon Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

46 Citations: Showing 1 - 10
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  1. 89Zr-atezolizumab imaging as a non-invasive approach to assess clinical response to PD-L1 blockade in cancer
    Authors: F Bensch, EL van der Ve, MN Lub-de Hoo, A Jorritsma-, R Boellaard, IC Kok, SF Oosting, CP Schröder, TJN Hiltermann, AJ van der We, HJM Groen, TC Kwee, SG Elias, JA Gietema, SS Bohorquez, A de Crespig, SP Williams, C Mancao, AH Brouwers, BM Fine, EGE de Vries
    Nat. Med., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cell
    Applications: Bioassay
  2. PLC?1 regulates SDF-1?-induced lymphocyte adhesion and migration to sites of inflammation
    Authors: M Strazza, I Azoulay-Al, M Peled, AV Smrcka, EY Skolnik, S Srivastava, A Mor
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis
    Authors: S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio
    Arthritis Res. Ther., 2017;19(1):97.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  4. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
    Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
    Sci Rep, 2017;7(1):6607.
    Species: Human
    Sample Types: Whole Cells
    Applications: Fuctional Assay
  5. ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function
    Authors: C Tao, H Shao, W Zhang, H Bo, F Wu, H Shen, S Huang
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue
    Authors: C Caër, C Rouault, T Le Roy, C Poitou, J Aron-Wisne, A Torcivia, JC Bichet, K Clément, M Guerre-Mil, S André
    Sci Rep, 2017;7(1):3000.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  7. Human trophoblast cells induced MDSCs from peripheral blood CD14(+) myelomonocytic cells via elevated levels of CCL2
    Authors: Xun Qu
    Cell. Mol. Immunol., 2016;13(5):615-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  8. Expression of adipokines in osteoarthritis osteophytes and their effect on osteoblasts
    Authors: Elena Neumann
    Matrix Biol., 2016;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation.
    Authors: Moore C, Cui Q, Warsi N, Durafourt B, Zorko N, Owen D, Antel J, Bar-Or A
    J Immunol, 2015;194(2):761-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  10. T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines.
    Authors: Huang W, Lu C, Wu Y, Ouyang S, Chen Y
    J Exp Clin Cancer Res, 2015;34(0):54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  11. PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation.
    Authors: Patsoukis N, Bardhan K, Chatterjee P, Sari D, Liu B, Bell L, Karoly E, Freeman G, Petkova V, Seth P, Li L, Boussiotis V
    Nat Commun, 2015;6(0):6692.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  12. Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.
    Authors: Fan, Miaomiao, Zhang, Yijun, Huang, Zhuoqion, Liu, Jun, Guo, Xuemin, Zhang, Hui, Luo, Haihua
    PLoS ONE, 2014;9(9):e108253.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  13. IkappaB kinase epsilon targets interferon regulatory factor 1 in activated T lymphocytes.
    Authors: Sgarbanti M, Marsili G, Remoli A, Stellacci E, Mai A, Rotili D, Perrotti E, Acchioni C, Orsatti R, Iraci N, Ferrari M, Borsetti A, Hiscott J, Battistini A
    Mol Cell Biol, 2014;34(6):1054-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  14. 1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients.
    Authors: Van Belle T, Vanherwegen A, Feyaerts D, De Clercq P, Verstuyf A, Korf H, Gysemans C, Mathieu C
    PLoS ONE, 2014;9(10):e109194.
  15. Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation.
    Authors: Zhu L, Huang Z, Stalesen R, Hansson G, Li N
    J Thromb Haemost, 2014;12(7):1156-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma.
    Authors: Ruan M, Thorn K, Liu S, Li S, Yang W, Zhang C, Zhang C
    Int J Oncol, 2014;44(6):2103-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func (T-cell isola
  17. A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1.
    Authors: Cevikbas F, Wang X, Akiyama T, Kempkes C, Savinko T, Antal A, Kukova G, Buhl T, Ikoma A, Buddenkotte J, Soumelis V, Feld M, Alenius H, Dillon S, Carstens E, Homey B, Basbaum A, Steinhoff M
    J Allergy Clin Immunol, 2014;133(2):448-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  18. CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
    Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
    Cell Stem Cell, 2014;14(3):342-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  19. Autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in gastric and colorectal cancer patients.
    Authors: Gao D, Li C, Xie X, Zhao P, Wei X, Sun W, Liu H, Alexandrou A, Jones J, Zhao R, Li J
    PLoS ONE, 2014;9(4):e93886.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow
  20. Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases.
    Authors: Wang, Feng, Xu, Zhiwen, Zhou, Jie, Lo, Wing-Sze, Lau, Ching-Fu, Nangle, Leslie A, Yang, Xiang-Le, Zhang, Mingjie, Schimmel, Paul
    J Biol Chem, 2013;288(41):29223-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  21. Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
    Authors: Renand A, Milpied P, Rossignol J, Bruneau J, Lemonnier F, Dussiot M, Coulon S, Hermine O
    PLoS ONE, 2013;8(12):e85589.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  22. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
    Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
    FASEB J., 2011;25(1):409-19.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  23. B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis.
    Authors: Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S
    J. Immunol., 2011;186(0):4518.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  24. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010;184(9):4880-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  25. Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
    Authors: Eikawa S, Ohue Y, Kitaoka K
    J. Immunol., 2010;185(11):6734-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  26. Phase I therapeutic trial of the HIV-1 Tat protein and long term follow-up.
    Authors: Longo O, Tripiciano A, Fiorelli V, Bellino S, Scoglio A, Collacchi B, Alvarez MJ, Francavilla V, Arancio A, Paniccia G, Lazzarin A, Tambussi G, Din CT, Visintini R, Narciso P, Antinori A, D'Offizi G, Giulianelli M, Carta M, Di Carlo A, Palamara G, Giuliani M, Laguardia ME, Monini P, Magnani M, Ensoli F, Ensoli B
    Vaccine, 2009;27(25):3306-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  27. Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
    Authors: Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K
    Genes Immun., 2009;10(8):702-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  28. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
    Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
    Blood, 2009;113(24):6120-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  29. Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
    Authors: Ni Z, Walcheck B
    Clin. Immunol., 2009;133(2):257-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  30. Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells.
    Authors: Hirosako S, Goto E, Fujii K, Tsumori K, Hirata N, Tsumura S, Kamohara H, Kohrogi H
    Clin. Exp. Immunol., 2009;155(2):266-74.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  31. Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
    Authors: Tran CN, Thacker SG, Louie DM, Oliver J, White PT, Endres JL, Urquhart AG, Chung KC, Fox DA
    J. Immunol., 2008;180(5):2989-98.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  32. Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
    Authors: Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U
    J. Immunol., 2007;179(6):4239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  33. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007;178(7):4184-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  34. Preferential migration of T regulatory cells induced by IL-16.
    Authors: McFadden C, Morgan R, Rahangdale S, Green D, Yamasaki H, Center D, Cruikshank W
    J. Immunol., 2007;179(10):6439-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  35. Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis.
    Authors: Mamoni RL, Blotta MH
    Cytokine, 2006;35(3):207-16.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  36. B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease.
    Authors: Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux NY, Goncalves RB, Valverde P, Dibart S, Li YP, Miranda LA, Ernst CW, Izumi Y, Taubman MA
    Am. J. Pathol., 2006;169(3):987-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: IP
  37. A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood.
    Authors: Markovic SN, Nevala WK, Uhl CB, Celis E, McKean DJ
    Clin. Exp. Immunol., 2006;145(3):438-47.
    Species: Human
    Sample Types:
    Applications: Binding Assay
  38. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005;46(6):659-67.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  39. Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay.
    Authors: Ogilvie RL, Abelson M, Hau HH, Vlasova I, Blackshear PJ, Bohjanen PR
    J. Immunol., 2005;174(2):953-61.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  40. Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
    Authors: Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY
    J. Orthop. Res., 2005;23(1):203-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004;21(2):241-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  42. HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis.
    Authors: Janardhan A, Swigut T, Hill B, Myers MP, Skowronski J
    PLoS Biol., 2004;2(1):E6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  43. Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor.
    Authors: Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM
    Biochem. J., 2004;380(0):425-33.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  44. In vitro differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
    Authors: Takahashi R, Mizukawa Y, Yamazaki Y, Hayakawa K, Hayakawa J, Kudo A, Shiohara T
    J. Immunol., 2003;171(11):5769-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Func
  45. NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
    Authors: Han MK, Kim JS, Park BH, Kim JR, Hwang BY, Lee HY, Song EK, Yoo WH
    J. Leukoc. Biol., 2003;73(4):525-9.
    Species: Feline
    Sample Types: Whole Cells
    Applications: Flow

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Human CD3 epsilon Antibody
By Anonymous on 04/18/2018
Application: IHC Sample Tested: Spleen tissue Species: Mouse

Human CD3 epsilon Antibody
By Anonymous on 04/13/2018
Application: IHC Sample Tested: Tumor cell lyastes Species: Human

Human CD3 epsilon Antibody
By Anonymous on 03/21/2018
Application: Flow Sample Tested: Blood mononuclear cells (PBMCs) Species: Human

Human CD3 epsilon Antibody
By Anonymous on 10/25/2017
Application: ELISA Sample Tested: Human recombinant antibody Species: Human