Human CD3 epsilon Antibody

Clone UCHT1 was used by HLDA to establish CD designation.
(43 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Recognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
  • Source
    Monoclonal Mouse IgG1 Clone # UCHT1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human thymocytes (1)
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunoprecipitation
    McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York
  • CyTOF-reported
    Behbehani, G.K. et al. (2015) Cancer Discov. 5: 988. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • T Cell Stimulation
    This antibody can be used to activate T cells when immobilized at 1-10 μg/mL (100 μL/well).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD3 epsilon in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Immunocytochemistry
CD3 epsilon in Human PBMCs. CD3 epsilon was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3 epsilon

CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.

  • References:
    1. Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
  • Entrez Gene IDs:
    916 (Human); 12503 (Mouse)
  • Alternate Names:
    CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Immune cell-derived cytokines contribute to obesity-related inflammation, fibrogenesis and metabolic deregulation in human adipose tissue
    Authors: C Caër, C Rouault, T Le Roy, C Poitou, J Aron-Wisne, A Torcivia, JC Bichet, K Clément, M Guerre-Mil, S André
    Sci Rep, 2017;7(1):3000.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. PLC?1 regulates SDF-1?-induced lymphocyte adhesion and migration to sites of inflammation
    Authors: M Strazza, I Azoulay-Al, M Peled, AV Smrcka, EY Skolnik, S Srivastava, A Mor
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. ??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function
    Authors: C Tao, H Shao, W Zhang, H Bo, F Wu, H Shen, S Huang
    Mol Med Rep, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. Interleukin-10-producing LAG3(+) regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis
    Authors: S Nakachi, S Sumitomo, Y Tsuchida, H Tsuchiya, M Kono, R Kato, K Sakurai, N Hanata, Y Nagafuchi, S Tateishi, H Kanda, T Okamura, K Yamamoto, K Fujio
    Arthritis Res. Ther., 2017;19(1):97.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  5. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
    Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
    Sci Rep, 2017;7(1):6607.
    Species: Human
    Sample Type: Whole Cells
    Application: Fuctional Assay
  6. T-type calcium channel antagonists, mibefradil and NNC-55-0396 inhibit cell proliferation and induce cell apoptosis in leukemia cell lines.
    Authors: Huang W, Lu C, Wu Y, Ouyang S, Chen Y
    J Exp Clin Cancer Res, 2015;34(0):54.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  7. Direct and indirect effects of immune and central nervous system-resident cells on human oligodendrocyte progenitor cell differentiation.
    Authors: Moore C, Cui Q, Warsi N, Durafourt B, Zorko N, Owen D, Antel J, Bar-Or A
    J Immunol, 2015;194(2):761-72.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  8. PD-1 alters T-cell metabolic reprogramming by inhibiting glycolysis and promoting lipolysis and fatty acid oxidation.
    Authors: Patsoukis N, Bardhan K, Chatterjee P, Sari D, Liu B, Bell L, Karoly E, Freeman G, Petkova V, Seth P, Li L, Boussiotis V
    Nat Commun, 2015;6(0):6692.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  9. A sensory neuron-expressed IL-31 receptor mediates T helper cell-dependent itch: Involvement of TRPV1 and TRPA1.
    Authors: Cevikbas F, Wang X, Akiyama T, Kempkes C, Savinko T, Antal A, Kukova G, Buhl T, Ikoma A, Buddenkotte J, Soumelis V, Feld M, Alenius H, Dillon S, Carstens E, Homey B, Basbaum A, Steinhoff M
    J Allergy Clin Immunol, 2014;133(2):448-60.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  10. Autologous tumor lysate-pulsed dendritic cell immunotherapy with cytokine-induced killer cells improves survival in gastric and colorectal cancer patients.
    Authors: Gao D, Li C, Xie X, Zhao P, Wei X, Sun W, Liu H, Alexandrou A, Jones J, Zhao R, Li J
    PLoS ONE, 2014;9(4):e93886.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. 1,25-Dihydroxyvitamin D3 and its analog TX527 promote a stable regulatory T cell phenotype in T cells from type 1 diabetes patients.
    Authors: Van Belle T, Vanherwegen A, Feyaerts D, De Clercq P, Verstuyf A, Korf H, Gysemans C, Mathieu C
    PLoS ONE, 2014;9(10):e109194.
  12. CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
    Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
    Cell Stem Cell, 2014;14(3):342-56.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  13. IkappaB kinase epsilon targets interferon regulatory factor 1 in activated T lymphocytes.
    Authors: Sgarbanti M, Marsili G, Remoli A, Stellacci E, Mai A, Rotili D, Perrotti E, Acchioni C, Orsatti R, Iraci N, Ferrari M, Borsetti A, Hiscott J, Battistini A
    Mol Cell Biol, 2014;34(6):1054-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  14. Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.
    Authors: Fan, Miaomiao, Zhang, Yijun, Huang, Zhuoqion, Liu, Jun, Guo, Xuemin, Zhang, Hui, Luo, Haihua
    PLoS ONE, 2014;9(9):e108253.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  15. The secretion of IL-6 by CpG-ODN-treated cancer cells promotes T-cell immune responses partly through the TLR-9/AP-1 pathway in oral squamous cell carcinoma.
    Authors: Ruan M, Thorn K, Liu S, Li S, Yang W, Zhang C, Zhang C
    Int J Oncol, 2014;44(6):2103-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay (T-cell isola
  16. Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation.
    Authors: Zhu L, Huang Z, Stalesen R, Hansson G, Li N
    J Thromb Haemost, 2014;12(7):1156-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  17. Regulated capture by exosomes of mRNAs for cytoplasmic tRNA synthetases.
    Authors: Wang, Feng, Xu, Zhiwen, Zhou, Jie, Lo, Wing-Sze, Lau, Ching-Fu, Nangle, Leslie A, Yang, Xiang-Le, Zhang, Mingjie, Schimmel, Paul
    J Biol Chem, 2013;288(41):29223-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  18. Neuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs.
    Authors: Renand A, Milpied P, Rossignol J, Bruneau J, Lemonnier F, Dussiot M, Coulon S, Hermine O
    PLoS ONE, 2013;8(12):e85589.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  19. Naive and activated T cells display differential responsiveness to TL1A that affects Th17 generation, maintenance, and proliferation.
    Authors: Jones GW, Stumhofer JS, Foster T, Twohig JP, Hertzog P, Topley N, Williams AS, Hunter CA, Jenkins BJ, Wang EC, Jones SA
    FASEB J., 2011;25(1):409-19.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  20. B cells as a therapeutic target for IFN-beta in relapsing-remitting multiple sclerosis.
    Authors: Ramgolam VS, Sha Y, Marcus KL, Choudhary N, Troiani L, Chopra M, Markovic-Plese S
    J. Immunol., 2011;186(0):4518.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  21. Enrichment of Foxp3+ CD4 regulatory T cells in migrated T cells to IL-6- and IL-8-expressing tumors through predominant induction of CXCR1 by IL-6.
    Authors: Eikawa S, Ohue Y, Kitaoka K
    J. Immunol., 2010;185(11):6734-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  22. IL-17 amplifies human contact hypersensitivity by licensing hapten nonspecific Th1 cells to kill autologous keratinocytes.
    Authors: Pennino D, Eyerich K, Scarponi C, Carbone T, Eyerich S, Nasorri F, Garcovich S, Traidl-Hoffmann C, Albanesi C, Cavani A
    J. Immunol., 2010;184(9):4880-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  23. Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
    Authors: Ni Z, Walcheck B
    Clin. Immunol., 2009;133(2):257-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  24. Human bronchial intraepithelial T cells produce interferon-gamma and stimulate epithelial cells.
    Authors: Hirosako S, Goto E, Fujii K, Tsumori K, Hirata N, Tsumura S, Kamohara H, Kohrogi H
    Clin. Exp. Immunol., 2009;155(2):266-74.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  25. Despite IFN-lambda receptor expression, blood immune cells, but not keratinocytes or melanocytes, have an impaired response to type III interferons: implications for therapeutic applications of these cytokines.
    Authors: Witte K, Gruetz G, Volk HD, Looman AC, Asadullah K, Sterry W, Sabat R, Wolk K
    Genes Immun., 2009;10(8):702-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  26. Phase I therapeutic trial of the HIV-1 Tat protein and long term follow-up.
    Authors: Longo O, Tripiciano A, Fiorelli V, Bellino S, Scoglio A, Collacchi B, Alvarez MJ, Francavilla V, Arancio A, Paniccia G, Lazzarin A, Tambussi G, Din CT, Visintini R, Narciso P, Antinori A, D'Offizi G, Giulianelli M, Carta M, Di Carlo A, Palamara G, Giuliani M, Laguardia ME, Monini P, Magnani M, Ensoli F, Ensoli B
    Vaccine, 2009;27(25):3306-12.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  27. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells.
    Authors: Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R
    Blood, 2009;113(24):6120-7.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  28. Interactions of T cells with fibroblast-like synoviocytes: role of the B7 family costimulatory ligand B7-H3.
    Authors: Tran CN, Thacker SG, Louie DM, Oliver J, White PT, Endres JL, Urquhart AG, Chung KC, Fox DA
    J. Immunol., 2008;180(5):2989-98.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  29. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007;178(7):4184-93.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  30. Interaction between transmembrane TNF and TNFR1/2 mediates the activation of monocytes by contact with T cells.
    Authors: Rossol M, Meusch U, Pierer M, Kaltenhauser S, Hantzschel H, Hauschildt S, Wagner U
    J. Immunol., 2007;179(6):4239-48.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  31. Preferential migration of T regulatory cells induced by IL-16.
    Authors: McFadden C, Morgan R, Rahangdale S, Green D, Yamasaki H, Center D, Cruikshank W
    J. Immunol., 2007;179(10):6439-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  32. B and T lymphocytes are the primary sources of RANKL in the bone resorptive lesion of periodontal disease.
    Authors: Kawai T, Matsuyama T, Hosokawa Y, Makihira S, Seki M, Karimbux NY, Goncalves RB, Valverde P, Dibart S, Li YP, Miranda LA, Ernst CW, Izumi Y, Taubman MA
    Am. J. Pathol., 2006;169(3):987-98.
    Species: Human
    Sample Type: Whole Cells
    Application: IP
  33. Flow-cytometric analysis of cytokine production in human paracoccidioidomycosis.
    Authors: Mamoni RL, Blotta MH
    Cytokine, 2006;35(3):207-16.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  34. A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood.
    Authors: Markovic SN, Nevala WK, Uhl CB, Celis E, McKean DJ
    Clin. Exp. Immunol., 2006;145(3):438-47.
    Species: Human
    Sample Type:
    Application: Binding Assay
  35. Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
    Authors: Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY
    J. Orthop. Res., 2005;23(1):203-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  36. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005;46(6):659-67.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  37. Tristetraprolin down-regulates IL-2 gene expression through AU-rich element-mediated mRNA decay.
    Authors: Ogilvie RL, Abelson M, Hau HH, Vlasova I, Blackshear PJ, Bohjanen PR
    J. Immunol., 2005;174(2):953-61.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  38. IL-22 increases the innate immunity of tissues.
    Authors: Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
    Immunity, 2004;21(2):241-54.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  39. HIV-1 Nef binds the DOCK2-ELMO1 complex to activate rac and inhibit lymphocyte chemotaxis.
    Authors: Janardhan A, Swigut T, Hill B, Myers MP, Skowronski J
    PLoS Biol., 2004;2(1):E6.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  40. Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor.
    Authors: Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM
    Biochem. J., 2004;380(0):425-33.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  41. NF-kappaB-dependent lymphocyte hyperadhesiveness to synovial fibroblasts by hypoxia and reoxygenation: potential role in rheumatoid arthritis.
    Authors: Han MK, Kim JS, Park BH, Kim JR, Hwang BY, Lee HY, Song EK, Yoo WH
    J. Leukoc. Biol., 2003;73(4):525-9.
    Species: Feline
    Sample Type: Whole Cells
    Application: Flow
  42. In vitro differentiation from naive to mature E-selectin binding CD4 T cells: acquisition of skin-homing properties occurs independently of cutaneous lymphocyte antigen expression.
    Authors: Takahashi R, Mizukawa Y, Yamazaki Y, Hayakawa K, Hayakawa J, Kudo A, Shiohara T
    J. Immunol., 2003;171(11):5769-77.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
Expand to show all 43 Citations
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ApplicationELISA
Sample TestedHuman recombinant antibody
SpeciesHuman

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