Human CD8 alpha Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CD8 alpha in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse CD8 alpha is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 37006
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human CD8 alpha
    Ser22-Val198
    Accession # NP_741969
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Human whole blood lymphocytes
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry

CD8 alpha in Human PBMCs. CD8 alpha was detected in immersion fixed human peripheral blood mononuclear cells using Mouse Anti-Human CD8 alpha Monoclonal Antibody (Catalog # MAB1509) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD8

CD8 is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes.

  • Entrez Gene IDs:
    925 (Human); 12525 (Mouse); 24930 (Rat); 493799 (Feline)
  • Alternate Names:
    CD8; CD8A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Kv1.3 channels mark functionally competent CD8+ tumor infiltrating lymphocytes in head and neck cancer
    Cancer Res., 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  2. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
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