Human Alkaline Phosphatase/ALPL Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).
  • Source
    Monoclonal Mouse IgG1 Clone # B4-78
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody
(Catalog # F0102B).
Immunocytochemistry
Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells. Alkaline Phosphatase/ALPL and Oct‑3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct‑3/4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; Catalog # NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
Alkaline Phosphatase/ALPL in Human Kidney. Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Alkaline Phosphatase/ALPL
The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.
  • References:
    1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
    2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
    3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.
  • Long Name:
    Alkaline Phosphatase Liver
  • Entrez Gene IDs:
    249 (Human); 11647 (Mouse)
  • Alternate Names:
    Akp2; Alkaline phosphatase liver/bone/kidney isozyme; alkaline phosphatase, liver/bone/kidney; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphomonoesterase; ALPL; APTNAP; AP-TNAP; EC 3.1.3.1; FLJ40094; FLJ93059; glycerophosphatase; HOPS; liver/bone/kidney-type alkaline phosphatase; MGC161443; tissue-nonspecific ALP; TNAP; TNSALP; TNSALPMGC167935
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. V-myc immortalizes human neural stem cells in the absence of pluripotency-associated traits.
    Authors: Pino-Barrio M, Garcia-Garcia E, Menendez P, Martinez-Serrano A
    PLoS ONE, 2015;10(3):e0118499.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. Osteodifferentiated mesenchymal stem cells from bone marrow and adipose tissue express HLA-G and display immunomodulatory properties in HLA-mismatched settings: implications in bone repair therapy.
    Authors: Montespan F, Deschaseaux F, Sensebe L, Carosella E, Rouas-Freiss N
    J Immunol Res, 2014;2014(0):230346.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Cementum- and periodontal ligament-like tissue formation by dental follicle cell sheets co-cultured with Hertwig's epithelial root sheath cells.
    Authors: Bai Y, Bai Y, Matsuzaka K, Hashimoto S, Fukuyama T, Wu L, Miwa T, Liu X, Wang X, Inoue T
    Bone, 2011;48(6):1417-26.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. In vivo impact of a 4 bp deletion mutation in the DLX3 gene on bone development.
    Authors: Choi SJ, Roodman GD, Feng JQ, Song IS, Amin K, Hart PS, Wright JT, Haruyama N, Hart TC
    Dev. Biol., 2009;325(1):129-37.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  5. Substance P stimulates bone marrow stromal cell osteogenic activity, osteoclast differentiation, and resorption activity in vitro.
    Authors: Wang L, Zhao R, Shi X, Wei T, Halloran BP, Clark DJ, Jacobs CR, Kingery WS
    Bone, 2009;45(2):309-20.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  6. The influence of proepicardial cells on the osteogenic potential of marrow stromal cells in a three-dimensional tubular scaffold.
    Authors: Valarmathi MT, Yost MJ, Goodwin RL, Potts JD
    Biomaterials, 2008;29(14):2203-16.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  7. Bone marrow-derived osteoblast progenitor cells in circulating blood contribute to ectopic bone formation in mice.
    Authors: Otsuru S, Tamai K, Yamazaki T, Yoshikawa H, Kaneda Y
    Biochem. Biophys. Res. Commun., 2007;354(2):453-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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