Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat, Primate - Callithrix jacchus (Common Marmoset)

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # B4-78
View all Alkaline Phosphatase/ALPL Primary Antibodies »

Product Specifications

Immunogen

Human liver, bone and kidney-derived Alkaline Phosphatase/ALPL

Specificity

Detects liver, bone and kidney Alkaline Phosphatase/ALPL from human tissue (2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Alkaline Phosphatase/ALPL Antibody

Detection of Alkaline Phosphatase/ALPL antibody in BG01V Human Cells antibody by Flow Cytometry.

Detection of Alkaline Phosphatase/ALPL in BG01V Human Cells by Flow Cytometry.

BG01V human embryonic stem cells was stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).
Alkaline Phosphatase and Oct‑3/4 antibody in BG01V Human Stem Cells by Immunocytochemistry (ICC).

Alkaline Phosphatase and Oct‑3/4 in BG01V Human Stem Cells.

Alkaline Phosphatase/ALPL and Oct-3/4 were detected in human BG01V embryonic stem cells using 10 µg/mL Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) and 10 µg/mL Human Oct-3/4 Antigen Affinity-purified Polyclonal Antibody (AF1759). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Alkaline Phosphatase/ALPL using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (pseudo-stained green; NL007), and stained for Oct-3/4 using the NorthernLights 637-conjugated Anti-Goat IgG Secondary Antibody (red; NL002). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Alkaline Phosphatase/ALPL antibody in Human Kidney by Immunohistochemistry (IHC-P).

Alkaline Phosphatase/ALPL in Human Kidney.

Alkaline Phosphatase/ALPL was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Alkaline Phosphatase/ALPL in Whole blood granulocytes by Flow Cytometry.

Whole blood granulocytes were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Detection of Human Alkaline Phosphatase/ALPL by Immunocytochemistry/Immunofluorescence

Detection of Human Alkaline Phosphatase/ALPL by Immunocytochemistry/Immunofluorescence

Immunocytochemistry of pluripotency markers.Staining was performed 48 hours following the last passage. From left to right: staining displayed by hESCs (HS181 line, low and high magnifications), hNPCs, v-myc immortalized lines (hNS1, hVM1, and hCTX) and fibroblasts (hFF-1). From top to bottom the markers studied were: NANOG, OCT3/4, SOX2 and AP. Hoechst 33258 nuclear staining is shown in blue. Scale bar represents 100 μm for hESCs in the left hand column (low magnification) and 25 μm for all the other microphotographs. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0118499), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Alkaline Phosphatase/ALPL in iPSCs by Flow Cytometry

iPSCs were stained with Mouse Anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody (Catalog # MAB1448, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Alkaline Phosphatase/ALPL Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: BG01V human embryonic stem cells, Whole blood granulocytes, or iPSCs.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver and kidney

Reviewed Applications

Read 5 reviews rated 5 using MAB1448 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Alkaline Phosphatase/ALPL

The liver, bone and kidney Alkaline Phosphatase, also known as tissue non-specific Alkaline Phosphatase, is a glycosyl phosphatidylinositol (GPI) anchored protein. Human liver/bone/kidney Alkaline Phosphatase shares 90% amino acid sequence homology with the mouse enzyme.

References

  1. Lawson, G.M. et al. (1985) Clin. Chem. 31:381.
  2. Gronthos, S. et al. (1999) J. Bone Miner. Res. 14:47.
  3. Dorheim, M.A. et al. (1993) J. Cell Physiol. 154:317.

Long Name

Alkaline Phosphatase Liver

Alternate Names

Akp2, AP-TNAP, HOPS, TNAP, TNSALP

Entrez Gene IDs

249 (Human); 11647 (Mouse)

Gene Symbol

ALPL

Additional Alkaline Phosphatase/ALPL Products

Product Documents for Human Alkaline Phosphatase/ALPL Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Alkaline Phosphatase/ALPL Antibody

For research use only

Citations for Human Alkaline Phosphatase/ALPL Antibody

Customer Reviews for Human Alkaline Phosphatase/ALPL Antibody (5)

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Showing  1 - 5 of 5 reviews Showing All
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  • Human Alkaline Phosphatase/ALPL Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: engineered ALPL cells
    Species: Human
    Verified Customer | Posted 09/13/2022
    MAB1448 works well in western blotting HEK293T cells transfected with ALPL. I used 1:1000 dilution, the exposure time of imaging is about 10 seconds.
    Human Alkaline Phosphatase/ALPL Antibody MAB1448
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • Human Alkaline Phosphatase/ALPL Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: engineered ALPL overexpressing cell line
    Species: Human
    Verified Customer | Posted 09/12/2022
    This antibody has low background with ALPL negative cells and good shift with engineered ALPL overexpressing cells in flow assays.
    Human Alkaline Phosphatase/ALPL Antibody MAB1448
  • Human Alkaline Phosphatase/ALPL Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Ovarian cancer tissue
    Species: Human
    Verified Customer | Posted 09/09/2021
    Human Alkaline Phosphatase/ALPL Antibody MAB1448
  • Human Alkaline Phosphatase/ALPL Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver cancer tissue
    Species: Human
    Verified Customer | Posted 02/16/2020
    Human Alkaline Phosphatase/ALPL Antibody MAB1448
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID 23514783
    Species: Human
    Verified Customer | Posted 02/10/2015

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Protocols

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