Mouse IL-12 p70 Quantikine ELISA Kit

(80 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL)
  • Sensitivity
    2.5 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant mouse IL-12 p70
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-12 p70 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-12 p70 in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 expressed in Sf 21 insect cells using a baculovirus expression vector and antibodies raised against recombinant mouse IL-12. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-12 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12 p70.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020364436
Mean28681932056230
Standard Deviation2.56.519.42.36.220
CV%8.99.610.111.511.18.7

Recovery

The recovery of mouse IL-12 p70 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 101 95-106
Serum (n=7) 97 88-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with various concentrations of mouse IL-12 p70 in each matrix were diluted with Calibrator Diluent and assayed.
Mouse IL-12 p70 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-12 p70

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

    • Long Name
      Interleukin 12 p70
    • Entrez Gene IDs
      3592 (Human); 16159 (Mouse); 84405 (Rat);
    • Alternate Names
      CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    80 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
      Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
      J. Immunol., 2017;0(0):.
      Species: Mouse
      Sample Type: Tissue Homogenates
    2. Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
      Authors: U Lampreht T, L Loiacono, M Cemazar, U Kamensek, VM Fazio, G Sersa, E Signori
      Mediators Inflamm., 2017;2017(0):5285890.
      Species: Mouse
      Sample Type: Serum
    3. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
      Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
      J Microbiol Immunol Infect, 2017;0(0):.
      Species: Mouse
      Sample Type: BALF
    4. Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model
      Authors: S Ishikawa, T Ishikawa, C Tezuka, K Asano, M Sunagawa, T Hisamitsu
      Evid Based Complement Alternat Med, 2017;2017(0):6054706.
    5. Microwave ablation combined with OK-432 induces Th1-type response and specific antitumor immunity in a murine model of breast cancer
      Authors: L Li, W Wang, H Pan, G Ma, X Shi, H Xie, X Liu, Q Ding, W Zhou, S Wang
      J Transl Med, 2017;15(1):23.
      Species: Mouse
      Sample Type: Plasma
    6. Subtilisin-like protease-1 secreted through type IV secretion system contributes to high virulence of Streptococcus suis 2
      Authors: Supeng Yin
      Sci Rep, 2016;6(0):27369.
      Species: Mouse
      Sample Type: Serum
    7. Epigenetic changes in bone marrow progenitor cells influence the inflammatory phenotype and alter wound healing in type 2 diabetes.
      Authors: Gallagher K, Joshi A, Carson W, Schaller M, Allen R, Mukerjee S, Kittan N, Feldman E, Henke P, Hogaboam C, Burant C, Kunkel S
      Diabetes, 2015;64(4):1420-30.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    8. The effects of protein malnutrition on the TNF-RI and NF-kappaB expression via the TNF-alpha signaling pathway.
      Authors: de Oliveira D, Hastreiter A, Mello A, de Oliveira Beltran J, Oliveira Santos E, Borelli P, Fock R
      Cytokine, 2014;69(2):218-25.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Prostaglandin E2 negatively regulates the production of inflammatory cytokines/chemokines and IL-17 in visceral leishmaniasis.
      Authors: Saha A, Biswas A, Srivastav S, Mukherjee M, Das P, Ukil A
      J Immunol, 2014;193(5):2330-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    10. Leishmania donovani targets tumor necrosis factor receptor-associated factor (TRAF) 3 for impairing TLR4-mediated host response.
      Authors: Gupta P, Giri J, Srivastav S, Chande A, Mukhopadhyaya R, Das P, Ukil A
      FASEB J, 2014;28(4):1756-68.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    11. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells.
      Authors: Guo Z, Wen Z, Qin A, Zhou Y, Liao Z, Liu Z, Liang Y, Ren T, Xu L
      J Immunol, 2013;190(8):4337-48.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    12. Enhanced immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to dendritic cells via Dectin-1 by incorporating beta-glucan.
      Authors: Lipinski T, Fitieh A, St Pierre J, Ostergaard H, Bundle D, Touret N
      J Immunol, 2013;190(8):4116-28.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    13. PGI(2) signaling inhibits antigen uptake and increases migration of immature dendritic cells.
      Authors: Toki S, Goleniewska K, Huckabee M, Zhou W, Newcomb D, FitzGerald G, Lawson W, Peebles R
      J Leukoc Biol, 2013;94(1):77-88.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    14. Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration.
      Authors: Maina, Virginia, Marrella, Veronica, Mantero, Stefano, Cassani, Barbara, Fontana, Elena, Anselmo, Achille, Del Prete, Annalisa, Sozzani, Silvano, Vezzoni, Paolo, Poliani, Pietro L, Villa, Anna
      J Leukoc Biol, 2013;94(6):1221-30.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    15. Evaluation of p21 promoter for interleukin 12 radiation induced transcriptional targeting in a mouse tumor model.
      Authors: Kamensek, Urska, Sersa, Gregor, Cemazar, Maja
      Mol Cancer, 2013;12(1):136.
      Species: Mouse
      Sample Type: Tissue Homogenates
    16. The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
      Authors: Calderon J, Maganto-Garcia E, Punzon C, Carrion J, Terhorst C, Fresno M
      PLoS Pathog., 2012;8(7):e1002799.
      Species: Mouse
      Sample Type: Serum
    17. IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis.
      Authors: Owens BM, Beattie L, Moore JW, Brown N, Mann JL, Dalton JE, Maroof A, Kaye PM
      PLoS Pathog., 2012;8(7):e1002827.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    18. Anti-inflammatory effects of FTY720 do not prevent neuronal cell loss in a rat model of optic neuritis.
      Authors: Rau CR, Hein K, Sattler MB, Kretzschmar B, Hillgruber C, McRae BL, Diem R, Bahr M
      Am. J. Pathol., 2012;178(4):1770-81.
      Species: Rat
      Sample Type: Tissue Homogenates
    19. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
      Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
      Am. J. Pathol., 2012;179(1):302-14.
      Species: Mouse
      Sample Type: Tissue Homogenates
    20. TLR-mediated distinct IFN-gamma/IL-10 pattern induces protective immunity against murine visceral leishmaniasis.
      Eur. J. Immunol., 2012;42(8):2087-99.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    21. Protein kinase C delta (PKCdelta)-extracellular signal-regulated kinase 1/2 (ERK1/2) signaling cascade regulates glycogen synthase kinase-3 (GSK-3) inhibition-mediated interleukin-10 (IL-10) expression in lipopolysaccharide (LPS)-induced endotoxemia.
      Authors: Noh KT, Son KH, Jung ID, Kang HK, Hwang SA, Lee WS, You JC, Park YM
      J. Biol. Chem., 2012;287(17):14226-33.
      Species: Mouse
      Sample Type: Serum
    22. Improved host defense against septic peritonitis in mice lacking MyD88 and TRIF is linked to a normal interferon response.
      Authors: Reim D, Rossmann-Bloeck T, Jusek G, Prazeres da Costa O, Holzmann B
      J. Leukoc. Biol., 2011;90(3):613-20.
      Species: Mouse
      Sample Type: Plasma
    23. The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines.
      Authors: Vultaggio A, Nencini F, Pratesi S, Maggi L, Guarna A, Annunziato F, Romagnani S, Parronchi P, Maggi E
      J. Immunol., 2011;186(8):4707-15.
      Species: Mouse
      Sample Type: Serum
    24. Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
      Authors: Wang H, Cheng F
      J. Immunol., 2011;186(0):3986.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    25. The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
      Authors: Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N
      J. Immunol., 2011;187(3):1333-46.
      Species: Mouse
      Sample Type: Serum
    26. Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
      Authors: Diao J, Zhao J, Winter E
      J. Immunol., 2011;186(9):5058-67.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    27. Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages.
      Authors: Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA
      J. Immunol., 2010;185(4):2044-50.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    28. Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
      Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
      Cytokine, 2010;49(3):256-63.
      Species: Mouse
      Sample Type: BALF
    29. Zoledronic acid impairs myeloid differentiation to tumour-associated macrophages in mesothelioma.
      Authors: Veltman JD, Lambers ME, van Nimwegen M
      Br. J. Cancer, 2010;103(5):629-41.
      Species: Mouse
      Sample Type: Peritoneal Fluid
    30. Oncostatin M induces dendritic cell maturation and Th1 polarization.
      Authors: Jung ID, Noh KT, Lee CM, Chun SH, Jeong SK, Park JW, Park WS, Kim HW, Yun CH, Shin YK, Park YM
      Biochem. Biophys. Res. Commun., 2010;394(2):272-8.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    31. Critical role of the disintegrin metalloprotease ADAM17 for intestinal inflammation and regeneration in mice.
      Authors: Chalaris A, Adam N, Sina C, Rosenstiel P, Lehmann-Koch J, Schirmacher P, Hartmann D, Cichy J, Gavrilova O, Schreiber S, Jostock T, Matthews V, Hasler R, Becker C, Neurath MF, Reiss K, Saftig P, Scheller J, Rose-John S
      J. Exp. Med., 2010;207(8):1617-24.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    32. Intravenous tolerance modulates macrophage classical activation and antigen presentation in experimental autoimmune encephalomyelitis.
      Authors: Li H, Ciric B, Yang J, Xu H, Fitzgerald DC, Elbehi M, Fonseca-Kelly Z, Yu S, Zhang GX, Rostami A
      J. Neuroimmunol., 2009;208(1):54-60.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    33. A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
      Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
      J. Immunol., 2009;183(2):1291-300.
      Species: Mouse
      Sample Type: BALF
    34. The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.
      Authors: Szymczak WA, Deepe GS
      J. Immunol., 2009;183(3):1964-74.
      Species: Mouse
      Sample Type: Tissue Homogenates
    35. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
      Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
      Vaccine, 2009;27(23):3045-52.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    36. Inhibition of monocyte-derived inflammatory cytokines by IL-25 occurs via p38 Map kinase-dependent induction of Socs-3.
      Authors: Caruso R, Stolfi C, Sarra M, Rizzo A, Fantini MC, Pallone F, MacDonald TT, Monteleone G
      Blood, 2009;113(15):3512-9.
      Species: Mouse
      Sample Type: Serum
    37. TLR9 activation coupled to IL-10 deficiency induces adverse pregnancy outcomes.
      Authors: Thaxton JE, Romero R, Sharma S
      J. Immunol., 2009;183(2):1144-54.
      Species: Mouse
      Sample Type: Serum
    38. Morphine withdrawal inhibits IL-12 induction in a macrophage cell line through a mechanism that involves cAMP.
      Authors: Kelschenbach J, Ninkovic J, Wang J, Krishnan A, Charboneau R, Barke RA, Roy S
      J. Immunol., 2008;180(6):3670-9.
      Species: Mouse
      Sample Type: Whole Cells
    39. The novel inosine analogue, INO-2002, protects against diabetes development in multiple low-dose streptozotocin and non-obese diabetic mouse models of type I diabetes.
      Authors: Mabley JG, Pacher P, Murthy KG, Williams W, Southan GJ, Salzman AL, Szabo C
      J. Endocrinol., 2008;198(3):581-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    40. Curcumin attenuates ovalbumin-induced airway inflammation by regulating nitric oxide.
      Authors: Moon DO, Kim MO, Lee HJ, Choi YH, Park YM, Heo MS, Kim GY
      Biochem. Biophys. Res. Commun., 2008;375(2):275-9.
      Species: Mouse
      Sample Type: BALF
    41. TLR-2 and IL-17A in chitin-induced macrophage activation and acute inflammation.
      Authors: Da Silva CA, Hartl D, Liu W, Lee CG, Elias JA
      J. Immunol., 2008;181(6):4279-86.
      Species: Mouse
      Sample Type: BALF
    42. Pulmonary stromal cells induce the generation of regulatory DC attenuating T-cell-mediated lung inflammation.
      Authors: Li Q, Guo Z, Xu X, Xia S, Cao X
      Eur. J. Immunol., 2008;38(10):2751-61.
      Species: Mouse
      Sample Type: BALF
    43. A tumor-selective biotherapy with prolonged impact on established metastases based on cytokine gene-engineered MSCs.
      Authors: Chen X, Lin X, Zhao J, Shi W, Zhang H, Wang Y, Kan B, DU L, Wang B, Wei Y, Liu Y, Zhao X
      Mol. Ther., 2008;16(4):749-56.
      Species: Mouse
      Sample Type: Serum
    44. Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity.
      Authors: Wells JW, Cowled CJ, Farzaneh F
      J. Immunol., 2008;181(5):3422-31.
      Species: Mouse
      Sample Type: Serum
    45. Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis.
      Authors: Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T
      J. Immunol., 2008;181(6):4208-18.
      Species: Mouse
      Sample Type: Peritoneal Lavage
    46. CD47 deficiency protects mice from lipopolysaccharide-induced acute lung injury and Escherichia coli pneumonia.
      Authors: Su X, Johansen M, Looney MR, Brown EJ, Matthay MA
      J. Immunol., 2008;180(10):6947-53.
      Species: Mouse
      Sample Type: BALF
    47. The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
      Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
      Nat. Immunol., 2008;9(9):1047-54.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    48. Dendritic cells derived from TBP-2-deficient mice are defective in inducing T cell responses.
      Authors: Son A, Nakamura H, Okuyama H, Oka S, Yoshihara E, Liu W, Matsuo Y, Kondo N, Masutani H, Ishii Y, Iyoda T, Inaba K, Yodoi J
      Eur. J. Immunol., 2008;38(5):1358-67.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    49. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
      Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
      Eur. J. Pharmacol., 2008;590(1):400-8.
      Species: Mouse
      Sample Type: Tissue Homogenates
    50. Dectin-1 interaction with Mycobacterium tuberculosis leads to enhanced IL-12p40 production by splenic dendritic cells.
      Authors: Rothfuchs AG, Bafica A, Feng CG, Egen JG, Williams DL, Brown GD, Sher A
      J. Immunol., 2007;179(6):3463-71.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    51. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
      Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
      Am. J. Pathol., 2007;170(4):1152-64.
      Species: Mouse
      Sample Type: Tissue Homogenates
    52. Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock.
      Authors: Vogl T, Tenbrock K, Ludwig S, Leukert N, Ehrhardt C, van Zoelen MA, Nacken W, Foell D, van der Poll T, Sorg C, Roth J
      Nat. Med., 2007;13(9):1042-9.
      Species: Mouse
      Sample Type: Serum
    53. Protectin D1 is generated in asthma and dampens airway inflammation and hyperresponsiveness.
      Authors: Levy BD, Kohli P, Gotlinger K, Haworth O, Hong S, Kazani S, Israel E, Haley KJ, Serhan CN
      J. Immunol., 2007;178(1):496-502.
      Species: Mouse
      Sample Type: BALF
    54. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
      Authors: Fujita H, Asahina A, Komine M, Tamaki K
      Cell. Immunol., 2007;245(2):70-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    55. Granulocyte-macrophage colony-stimulating factor prevents diabetes development in NOD mice by inducing tolerogenic dendritic cells that sustain the suppressive function of CD4+CD25+ regulatory T cells.
      Authors: Gaudreau S, Guindi C, Menard M, Besin G, Dupuis G, Amrani A
      J. Immunol., 2007;179(6):3638-47.
    56. IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3.
      Authors: Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S
      J. Immunol., 2007;178(12):7607-15.
      Species: Mouse
      Sample Type: Serum
    57. The immunomodulation of endotoxin-induced acute lung injury by hesperidin in vivo and in vitro.
      Authors: Yeh CC, Kao SJ, Lin CC, Wang SD, Liu CJ, Kao ST
      Life Sci., 2007;80(20):1821-31.
      Species: Mouse
      Sample Type: BALF
    58. CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis.
      Authors: Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA
      Am. J. Respir. Crit. Care Med., 2007;177(3):301-8.
      Species: Mouse
      Sample Type: Plasma
    59. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
      Authors: Lin YL, Liang YC, Chiang BL
      J. Leukoc. Biol., 2007;82(6):1473-80.
      Species: Human
      Sample Type: Cell Culture Supernates
    60. Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
      Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
      Blood, 2007;111(1):219-28.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    61. CD27 contributes to the early systemic immune response to Mycobacterium tuberculosis infection but does not affect outcome.
      Authors: Wieland CW, Kerver ME, Florquin S, Nolte MA, Borst J, van Lier R, van Oers MH, van der Poll T
      Int. Immunol., 2006;18(11):1531-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    62. Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
      Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
      Eur. Respir. J., 2006;28(2):275-85.
      Species: Mouse
      Sample Type: BALF
    63. Adenosine A2A receptor inactivation increases survival in polymicrobial sepsis.
      Authors: Nemeth ZH, Csoka B, Wilmanski J, Xu D, Lu Q, Ledent C, Deitch EA, Pacher P, Spolarics Z, Hasko G
      J. Immunol., 2006;176(9):5616-26.
      Species: Mouse
      Sample Type: Plasma
    64. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
      Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
      J. Immunol., 2005;174(4):2327-35.
      Species: Rat
      Sample Type: Cell Culture Supernates
    65. Endogenous interleukin-12 improves the early antimicrobial host response to murine Escherichia coli peritonitis.
      Authors: Weijer S, Florquin S, van der Poll T
      Shock, 2005;23(1):54-8.
      Species: Mouse
      Sample Type: Peritoneal Fluid
    66. Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
      Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
      Vaccine, 2005;24(9):1470-7.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    67. Vaccination of apoptotic Fas ligand-expressing tumors decreased antitumor responses by enhanced production of immunosuppressive cytokines.
      Authors: Wada A, Tada Y, Shimozato O, Takiguchi Y, Tatsumi K, Kuriyama T, Tagawa M
      Anticancer Res., 2005;25(1):299-303.
      Species: Mouse
      Sample Type: Tissue Secretion
    68. MyD88-dependent and -independent murine cytomegalovirus sensing for IFN-alpha release and initiation of immune responses in vivo.
      Authors: Delale T, Paquin A, Asselin-Paturel C, Dalod M, Brizard G, Bates EE, Kastner P, Chan S, Akira S, Vicari A, Biron CA, Trinchieri G, Briere F
      J. Immunol., 2005;175(10):6723-32.
      Species: Mouse
      Sample Type: Serum
    69. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
      Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
      Comp. Immunol. Microbiol. Infect. Dis., 2004;27(6):393-411.
      Species: Mouse
      Sample Type: Serum
    70. Gamma interferon does not enhance clearance of Pseudomonas aeruginosa but does amplify a proinflammatory response in a murine model of postseptic immunosuppression.
      Authors: Murphey ED, Herndon DN, Sherwood ER
      Infect. Immun., 2004;72(12):6892-901.
      Species: Mouse
      Sample Type: Plasma
    71. Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
      Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
      J. Immunol., 2004;172(3):1754-62.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    72. Effects of poly(ADP-ribose) polymerase inhibition on inflammatory cell migration in a murine model of asthma.
      Authors: Virag L, Bai P, Bak I, Pacher P, Mabley JG, Liaudet L, Bakondi E, Gergely P, Kollai M, Szabo C
      Med. Sci. Monit., 2004;10(3):BR77-83.
      Species: Mouse
      Sample Type: BALF
    73. Increased and long-term generation of dendritic cells with reduced function from IL-6-deficient bone marrow.
      Authors: Bleier JI, Pillarisetty VG, Shah AB, DeMatteo RP
      J. Immunol., 2004;172(12):7408-16.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    74. A protective role of locally administered immunostimulatory CpG oligodeoxynucleotide in a mouse model of genital herpes infection.
      Authors: Harandi AM, Eriksson K, Holmgren J
      J. Virol., 2003;77(2):953-62.
      Species: Mouse
      Sample Type: Serum
    75. Cutting edge: myeloid differentiation factor 88 deficiency improves resistance against sepsis caused by polymicrobial infection.
      Authors: Weighardt H, Kaiser-Moore S, Vabulas RM, Kirschning CJ, Wagner H, Holzmann B
      J. Immunol., 2002;169(6):2823-7.
      Species: Mouse
      Sample Type: Serum
    76. A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
      Authors: Huaux F, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
      J. Immunol., 2002;169(5):2653-61.
      Species: Mouse
      Sample Type: Tissue Homogenates
    77. Endogenous granulocyte-macrophage colony-stimulating factor overexpression in vivo results in the long-term recruitment of a distinct dendritic cell population with enhanced immunostimulatory function.
      Authors: Miller G, Pillarisetty VG, Shah AB, Lahrs S, Xing Z, DeMatteo RP
      J. Immunol., 2002;169(6):2875-85.
      Species: Mouse
      Sample Type: Serum
    78. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
      Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
      Eur. J. Immunol., 2001;31(7):2104-15.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    79. In vitro and in vivo immunostimulatory potential of bone marrow-derived mast cells on B- and T-lymphocyte activation.
      Authors: Tkaczyk C, Villa I, Peronet R, David B, Chouaib S, Mecheri S
      J. Allergy Clin. Immunol., 2000;105(1):134-42.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    80. Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans.
      Authors: de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A
      Infect. Immun., 2000;68(6):3297-304.
      Species: Rat
      Sample Type: Vaginal Fluid
    Expand to show all 80 Citations
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