Mouse IL-12 p70 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
PM1270
SM1270
M1270
Mouse IL-12 p70 ELISA Standard Curve
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Product Details
Procedure
Citations (95)
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Supplemental Products
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Mouse IL-12 p70 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL)
Sensitivity
2.5 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum)
Specificity
Natural and recombinant mouse IL-12 p70
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-12 p70 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-12 p70 in cell culture supernates and mouse serum. It contains recombinant mouse IL-12 expressed in Sf 21 insect cells using a baculovirus expression vector and antibodies raised against recombinant mouse IL-12. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-12 showed linear curves that were parallel to the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-12 p70.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 36 44 36
Mean 28 68 193 20 56 230
Standard Deviation 2.5 6.5 19.4 2.3 6.2 20
CV% 8.9 9.6 10.1 11.5 11.1 8.7

Recovery

The recovery of mouse IL-12 p70 spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=7) 101 95-106
Serum (n=7) 97 88-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with various concentrations of mouse IL-12 p70 in each matrix were diluted with Calibrator Diluent and assayed.
Mouse IL-12 p70 ELISA Linearity

Data Examples

Mouse IL-12 p70 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-12 p70

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Long Name:
Interleukin 12 p70
Entrez Gene IDs:
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names:
CLMF p35; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL12 p70; IL-12 p70; IL-12 subunit p35; IL-12, subunit p35; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-12 p70 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

95 Citations: Showing 1 - 10
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  1. Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population
    Authors: R Leylek, M Alcántara-, Z Lanzar, A Lüdtke, OA Perez, B Reizis, J Idoyaga
    Cell Rep, 2019;29(11):3736-3750.e8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Sphingosine-1-Phosphate Receptor 1 Activity Promotes Tumor Growth by Amplifying VEGF-VEGFR2 Angiogenic Signaling
    Authors: VA Balaji Rag, M Anwar, MZ Akhter, A Chavez, Y Mao, V Natarajan, S Lakshmikan, M Chrzanowsk, AZ Dudek, L Claesson-W, JK Kitajewski, KK Wary, AB Malik, D Mehta
    Cell Rep, 2019;29(11):3472-3487.e4.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Downregulation of RalGTPase-activating protein promotes colitis-associated cancer via NLRP3 inflammasome activation
    Authors: T Iida, Daisuke Hi, N Minami, M Matsuura, K Wagatsuma, K Kawakami, K Nagaishi, M Nojima, H Ikeuchi, S Hirota, R Shirakawa, H Horiuchi, H Nakase
    Cell Mol Gastroenterol Hepatol, 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Oncolytic activity of HF10 in head and neck squamous cell carcinomas
    Authors: S Esaki, F Goshima, H Ozaki, G Takano, Y Hatano, D Kawakita, K Ijichi, T Watanabe, Y Sato, T Murata, H Iwata, Y Shibamoto, S Murakami, Y Nishiyama, H Kimura
    Cancer Gene Ther., 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Emodin regulates neutrophil phenotypes to prevent hypercoagulation and lung carcinogenesis
    Authors: Z Li, Y Lin, S Zhang, L Zhou, G Yan, Y Wang, M Zhang, M Wang, H Lin, Q Tong, Y Duan, G Du
    J Transl Med, 2019;17(1):90.
    Species: Mouse
    Sample Types: Alveolar Fluid
  6. Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
    Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
    Redox Biol, 2019;22(0):101116.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  7. Dendritic cells modulate c-kit expression on the edge between activation and death
    Authors: S Simonetti, ABB Seijas, A Natalini, S Vitale, D Runci, A Soriani, A Di Virgili, E Aricò, L Gabriele, A Santoni, F Di Rosa
    Eur. J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Intratumoral expression using a NFkB-based promoter enhances IL12 antitumor efficacy
    Authors: G Garaulet, G Pérez-Chac, H Alarcón, A Alfranca, F Mulero, J Martínez-T, JM Zapata, A Rodríguez
    Cancer Gene Ther., 2019;0(0):.
    Species: Mouse
    Sample Types: Serum
  9. Non-replicating Newcastle Disease Virus as an adjuvant for DNA vaccine enhances antitumor efficacy through the induction of TRAIL and granzyme B expression
    Authors: A Mohebbi, MS Ebrahimzad, S Baghban Ra, M Saeidi, A Tabarraei, SR Mohebbi, S Shirian, A Gorji, A Ghaemi
    Virus Res., 2018;261(0):72-80.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. Cutting Edge: Quantitative Determination of CD40L Threshold for IL-12 and IL-23 Production from Dendritic Cells
    Authors: K Abdi, K Laky, K Padhan, C Petrovas, J Skinner, J Kabat, DW Dorward, J Brzostowsk, EO Long, G Trinchieri, R Varma
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. ?-Synuclein disrupts the anti-inflammatory role of Drd2 via interfering ?-arrestin2-TAB1 interaction in astrocytes
    Authors: RH Du, Y Zhou, ML Xia, M Lu, JH Ding, G Hu
    J Neuroinflammation, 2018;15(1):258.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Regulated intratumoral expression of IL-12 using a RheoSwitch Therapeutic System� (RTS�) gene switch as gene therapy for the treatment of glioma
    Authors: JA Barrett, H Cai, J Miao, PD Khare, P Gonzalez, J Dalsing-He, G Sharma, T Chan, LJN Cooper, F Lebel
    Cancer Gene Ther., 2018;0(0):.
    Species: Mouse
    Sample Types: Serum
  13. Carnosic acid protects mice from high-fat diet-induced NAFLD by regulating MARCKS
    Authors: HM Song, X Li, YY Liu, WP Lu, ZH Cui, L Zhou, D Yao, HM Zhang
    Int. J. Mol. Med., 2018;0(0):.
    Species: Mouse
    Sample Types: Serum
  14. Gene Electrotransfer of Plasmid-Encoding IL-12 Recruits the M1 Macrophages and Antigen-Presenting Cells Inducing the Eradication of Aggressive B16F10 Murine Melanoma
    Authors: U Lampreht T, L Loiacono, M Cemazar, U Kamensek, VM Fazio, G Sersa, E Signori
    Mediators Inflamm., 2017;2017(0):5285890.
    Species: Mouse
    Sample Types: Serum
  15. Efficacy of Juzentaihoto for Tumor Immunotherapy in B16 Melanoma Metastasis Model
    Authors: S Ishikawa, T Ishikawa, C Tezuka, K Asano, M Sunagawa, T Hisamitsu
    Evid Based Complement Alternat Med, 2017;2017(0):6054706.
  16. Microwave ablation combined with OK-432 induces Th1-type response and specific antitumor immunity in a murine model of breast cancer
    Authors: L Li, W Wang, H Pan, G Ma, X Shi, H Xie, X Liu, Q Ding, W Zhou, S Wang
    J Transl Med, 2017;15(1):23.
    Species: Mouse
    Sample Types: Plasma
  17. Fungal immunomodulatory protein-fve could modulate airway remodel through by affect IL17 cytokine
    Authors: YT Lee, CT Wu, HL Sun, JL Ko, KH Lue
    J Microbiol Immunol Infect, 2017;0(0):.
    Species: Mouse
    Sample Types: BALF
  18. Topical use and systemic action of green and roasted coffee oils and ground oils in a cutaneous incision model in rats (Rattus norvegicus albinus)
    Authors: BG Lania, J Morari, AL Souza, MND Silva, AR de Almeida, G Veira-Dami, SM Alegre, CL César, LA Velloso, ML Cintra, NB Maia, PENF Velho
    PLoS ONE, 2017;12(12):e0188779.
    Species: Rat
    Sample Types: Serum
  19. STAT1 Represses Cytokine-Producing Group 2 and Group 3 Innate Lymphoid Cells during Viral Infection
    Authors: MT Stier, K Goleniewsk, JY Cephus, DC Newcomb, TP Sherrill, KL Boyd, MH Bloodworth, ML Moore, K Chen, JK Kolls, RS Peebles
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. Tailor-made fibroblast-specific and antibiotic-free interleukin 12 plasmid for gene electrotransfer-mediated cancer immunotherapy
    Authors: Maja Cemazar
    Plasmid, 2016;89(0):9-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Subtilisin-like protease-1 secreted through type IV secretion system contributes to high virulence of Streptococcus suis 2
    Authors: Supeng Yin
    Sci Rep, 2016;6(0):27369.
    Species: Mouse
    Sample Types: Serum
  22. Epigenetic changes in bone marrow progenitor cells influence the inflammatory phenotype and alter wound healing in type 2 diabetes.
    Authors: Gallagher K, Joshi A, Carson W, Schaller M, Allen R, Mukerjee S, Kittan N, Feldman E, Henke P, Hogaboam C, Burant C, Kunkel S
    Diabetes, 2015;64(4):1420-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  23. Leishmania donovani targets tumor necrosis factor receptor-associated factor (TRAF) 3 for impairing TLR4-mediated host response.
    Authors: Gupta P, Giri J, Srivastav S, Chande A, Mukhopadhyaya R, Das P, Ukil A
    FASEB J, 2014;28(4):1756-68.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Prostaglandin E2 negatively regulates the production of inflammatory cytokines/chemokines and IL-17 in visceral leishmaniasis.
    Authors: Saha A, Biswas A, Srivastav S, Mukherjee M, Das P, Ukil A
    J Immunol, 2014;193(5):2330-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. The effects of protein malnutrition on the TNF-RI and NF-kappaB expression via the TNF-alpha signaling pathway.
    Authors: de Oliveira D, Hastreiter A, Mello A, de Oliveira Beltran J, Oliveira Santos E, Borelli P, Fock R
    Cytokine, 2014;69(2):218-25.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  26. PGI(2) signaling inhibits antigen uptake and increases migration of immature dendritic cells.
    Authors: Toki S, Goleniewska K, Huckabee M, Zhou W, Newcomb D, FitzGerald G, Lawson W, Peebles R
    J Leukoc Biol, 2013;94(1):77-88.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. Antisense oligonucleotide treatment enhances the recovery of acute lung injury through IL-10-secreting M2-like macrophage-induced expansion of CD4+ regulatory T cells.
    Authors: Guo Z, Wen Z, Qin A, Zhou Y, Liao Z, Liu Z, Liang Y, Ren T, Xu L
    J Immunol, 2013;190(8):4337-48.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  28. Evaluation of p21 promoter for interleukin 12 radiation induced transcriptional targeting in a mouse tumor model.
    Authors: Kamensek, Urska, Sersa, Gregor, Cemazar, Maja
    Mol Cancer, 2013;12(1):136.
    Species: Mouse
    Sample Types: Tissue Homogenates
  29. Enhanced immunogenicity of a tricomponent mannan tetanus toxoid conjugate vaccine targeted to dendritic cells via Dectin-1 by incorporating beta-glucan.
    Authors: Lipinski T, Fitieh A, St Pierre J, Ostergaard H, Bundle D, Touret N
    J Immunol, 2013;190(8):4116-28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. Hypomorphic mutation in the RAG2 gene affects dendritic cell distribution and migration.
    Authors: Maina, Virginia, Marrella, Veronica, Mantero, Stefano, Cassani, Barbara, Fontana, Elena, Anselmo, Achille, Del Prete, Annalisa, Sozzani, Silvano, Vezzoni, Paolo, Poliani, Pietro L, Villa, Anna
    J Leukoc Biol, 2013;94(6):1221-30.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
    Authors: Yamamoto Y, Otani S, Hirai H, Nagata K, Aritake K, Urade Y, Narumiya S, Yokozeki H, Nakamura M, Satoh T
    Am. J. Pathol., 2012;179(1):302-14.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. Anti-inflammatory effects of FTY720 do not prevent neuronal cell loss in a rat model of optic neuritis.
    Authors: Rau CR, Hein K, Sattler MB, Kretzschmar B, Hillgruber C, McRae BL, Diem R, Bahr M
    Am. J. Pathol., 2012;178(4):1770-81.
    Species: Rat
    Sample Types: Tissue Homogenates
  33. TLR-mediated distinct IFN-gamma/IL-10 pattern induces protective immunity against murine visceral leishmaniasis.
    Eur. J. Immunol., 2012;42(8):2087-99.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  34. Protein kinase C delta (PKCdelta)-extracellular signal-regulated kinase 1/2 (ERK1/2) signaling cascade regulates glycogen synthase kinase-3 (GSK-3) inhibition-mediated interleukin-10 (IL-10) expression in lipopolysaccharide (LPS)-induced endotoxemia.
    Authors: Noh KT, Son KH, Jung ID, Kang HK, Hwang SA, Lee WS, You JC, Park YM
    J. Biol. Chem., 2012;287(17):14226-33.
    Species: Mouse
    Sample Types: Serum
  35. The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
    Authors: Calderon J, Maganto-Garcia E, Punzon C, Carrion J, Terhorst C, Fresno M
    PLoS Pathog., 2012;8(7):e1002799.
    Species: Mouse
    Sample Types: Serum
  36. IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c+ Cell Populations during Visceral Leishmaniasis.
    Authors: Owens BM, Beattie L, Moore JW, Brown N, Mann JL, Dalton JE, Maroof A, Kaye PM
    PLoS Pathog., 2012;8(7):e1002827.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  37. Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10.
    Authors: Wang H, Cheng F
    J. Immunol., 2011;186(0):3986.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
    Authors: Diao J, Zhao J, Winter E
    J. Immunol., 2011;186(9):5058-67.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines.
    Authors: Vultaggio A, Nencini F, Pratesi S, Maggi L, Guarna A, Annunziato F, Romagnani S, Parronchi P, Maggi E
    J. Immunol., 2011;186(8):4707-15.
    Species: Mouse
    Sample Types: Serum
  40. The interaction between IL-18 and IL-18 receptor limits the magnitude of protective immunity and enhances pathogenic responses following infection with intracellular bacteria.
    Authors: Ghose P, Ali AQ, Fang R, Forbes D, Ballard B, Ismail N
    J. Immunol., 2011;187(3):1333-46.
    Species: Mouse
    Sample Types: Serum
  41. Improved host defense against septic peritonitis in mice lacking MyD88 and TRIF is linked to a normal interferon response.
    Authors: Reim D, Rossmann-Bloeck T, Jusek G, Prazeres da Costa O, Holzmann B
    J. Leukoc. Biol., 2011;90(3):613-20.
    Species: Mouse
    Sample Types: Plasma
  42. Critical role of the disintegrin metalloprotease ADAM17 for intestinal inflammation and regeneration in mice.
    Authors: Chalaris A, Adam N, Sina C, Rosenstiel P, Lehmann-Koch J, Schirmacher P, Hartmann D, Cichy J, Gavrilova O, Schreiber S, Jostock T, Matthews V, Hasler R, Becker C, Neurath MF, Reiss K, Saftig P, Scheller J, Rose-John S
    J. Exp. Med., 2010;207(8):1617-24.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  43. Zoledronic acid impairs myeloid differentiation to tumour-associated macrophages in mesothelioma.
    Authors: Veltman JD, Lambers ME, van Nimwegen M
    Br. J. Cancer, 2010;103(5):629-41.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  44. Proinflammatory clearance of apoptotic neutrophils induces an IL-12(low)IL-10(high) regulatory phenotype in macrophages.
    Authors: Filardy AA, Pires DR, Nunes MP, Takiya CM, Freire-de-Lima CG, Ribeiro-Gomes FL, DosReis GA
    J. Immunol., 2010;185(4):2044-50.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  45. Oncostatin M induces dendritic cell maturation and Th1 polarization.
    Authors: Jung ID, Noh KT, Lee CM, Chun SH, Jeong SK, Park JW, Park WS, Kim HW, Yun CH, Shin YK, Park YM
    Biochem. Biophys. Res. Commun., 2010;394(2):272-8.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Varying susceptibility of pulmonary cytokine production to lipopolysaccharide in mice.
    Authors: Alm AS, Li K, Yang D, Andersson R, Lu Y, Wang X
    Cytokine, 2010;49(3):256-63.
    Species: Mouse
    Sample Types: BALF
  47. A MyD88-dependent early IL-17 production protects mice against airway infection with the obligate intracellular pathogen Chlamydia muridarum.
    Authors: Zhang X, Gao L, Lei L, Zhong Y, Dube P, Berton MT, Arulanandam B, Zhang J, Zhong G
    J. Immunol., 2009;183(2):1291-300.
    Species: Mouse
    Sample Types: BALF
  48. Inhibition of monocyte-derived inflammatory cytokines by IL-25 occurs via p38 Map kinase-dependent induction of Socs-3.
    Authors: Caruso R, Stolfi C, Sarra M, Rizzo A, Fantini MC, Pallone F, MacDonald TT, Monteleone G
    Blood, 2009;113(15):3512-9.
    Species: Mouse
    Sample Types: Serum
  49. The CCL7-CCL2-CCR2 axis regulates IL-4 production in lungs and fungal immunity.
    Authors: Szymczak WA, Deepe GS
    J. Immunol., 2009;183(3):1964-74.
    Species: Mouse
    Sample Types: Tissue Homogenates
  50. TLR9 agonist, but not TLR7/8, functions as an adjuvant to diminish FI-RSV vaccine-enhanced disease, while either agonist used as therapy during primary RSV infection increases disease severity.
    Authors: Johnson TR, Rao S, Seder RA, Chen M, Graham BS
    Vaccine, 2009;27(23):3045-52.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  51. TLR9 activation coupled to IL-10 deficiency induces adverse pregnancy outcomes.
    Authors: Thaxton JE, Romero R, Sharma S
    J. Immunol., 2009;183(2):1144-54.
    Species: Mouse
    Sample Types: Serum
  52. Intravenous tolerance modulates macrophage classical activation and antigen presentation in experimental autoimmune encephalomyelitis.
    Authors: Li H, Ciric B, Yang J, Xu H, Fitzgerald DC, Elbehi M, Fonseca-Kelly Z, Yu S, Zhang GX, Rostami A
    J. Neuroimmunol., 2009;208(1):54-60.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  53. TLR-2 and IL-17A in chitin-induced macrophage activation and acute inflammation.
    Authors: Da Silva CA, Hartl D, Liu W, Lee CG, Elias JA
    J. Immunol., 2008;181(6):4279-86.
    Species: Mouse
    Sample Types: BALF
  54. CD47 deficiency protects mice from lipopolysaccharide-induced acute lung injury and Escherichia coli pneumonia.
    Authors: Su X, Johansen M, Looney MR, Brown EJ, Matthay MA
    J. Immunol., 2008;180(10):6947-53.
    Species: Mouse
    Sample Types: BALF
  55. Dendritic cells derived from TBP-2-deficient mice are defective in inducing T cell responses.
    Authors: Son A, Nakamura H, Okuyama H, Oka S, Yoshihara E, Liu W, Matsuo Y, Kondo N, Masutani H, Ishii Y, Iyoda T, Inaba K, Yodoi J
    Eur. J. Immunol., 2008;38(5):1358-67.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  56. Essential involvement of CX3CR1-mediated signals in the bactericidal host defense during septic peritonitis.
    Authors: Ishida Y, Hayashi T, Goto T, Kimura A, Akimoto S, Mukaida N, Kondo T
    J. Immunol., 2008;181(6):4208-18.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  57. The novel inosine analogue, INO-2002, protects against diabetes development in multiple low-dose streptozotocin and non-obese diabetic mouse models of type I diabetes.
    Authors: Mabley JG, Pacher P, Murthy KG, Williams W, Southan GJ, Salzman AL, Szabo C
    J. Endocrinol., 2008;198(3):581-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  58. A tumor-selective biotherapy with prolonged impact on established metastases based on cytokine gene-engineered MSCs.
    Authors: Chen X, Lin X, Zhao J, Shi W, Zhang H, Wang Y, Kan B, DU L, Wang B, Wei Y, Liu Y, Zhao X
    Mol. Ther., 2008;16(4):749-56.
    Species: Mouse
    Sample Types: Serum
  59. Curcumin attenuates ovalbumin-induced airway inflammation by regulating nitric oxide.
    Authors: Moon DO, Kim MO, Lee HJ, Choi YH, Park YM, Heo MS, Kim GY
    Biochem. Biophys. Res. Commun., 2008;375(2):275-9.
    Species: Mouse
    Sample Types: BALF
  60. The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
    Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
    Nat. Immunol., 2008;9(9):1047-54.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  61. Antifibrotic action of pirfenidone and prednisolone: different effects on pulmonary cytokines and growth factors in bleomycin-induced murine pulmonary fibrosis.
    Authors: Oku H, Shimizu T, Kawabata T, Nagira M, Hikita I, Ueyama A, Matsushima S, Torii M, Arimura A
    Eur. J. Pharmacol., 2008;590(1):400-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
  62. Morphine withdrawal inhibits IL-12 induction in a macrophage cell line through a mechanism that involves cAMP.
    Authors: Kelschenbach J, Ninkovic J, Wang J, Krishnan A, Charboneau R, Barke RA, Roy S
    J. Immunol., 2008;180(6):3670-9.
    Species: Mouse
    Sample Types: Whole Cells
  63. Combined triggering of dendritic cell receptors results in synergistic activation and potent cytotoxic immunity.
    Authors: Wells JW, Cowled CJ, Farzaneh F
    J. Immunol., 2008;181(5):3422-31.
    Species: Mouse
    Sample Types: Serum
  64. Pulmonary stromal cells induce the generation of regulatory DC attenuating T-cell-mediated lung inflammation.
    Authors: Li Q, Guo Z, Xu X, Xia S, Cao X
    Eur. J. Immunol., 2008;38(10):2751-61.
    Species: Mouse
    Sample Types: BALF
  65. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
    Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
    Am. J. Pathol., 2007;170(4):1152-64.
    Species: Mouse
    Sample Types: Tissue Homogenates
  66. The immunomodulation of endotoxin-induced acute lung injury by hesperidin in vivo and in vitro.
    Authors: Yeh CC, Kao SJ, Lin CC, Wang SD, Liu CJ, Kao ST
    Life Sci., 2007;80(20):1821-31.
    Species: Mouse
    Sample Types: BALF
  67. IL-27 synthesis induced by TLR ligation critically depends on IFN regulatory factor 3.
    Authors: Molle C, Nguyen M, Flamand V, Renneson J, Trottein F, De Wit D, Willems F, Goldman M, Goriely S
    J. Immunol., 2007;178(12):7607-15.
    Species: Mouse
    Sample Types: Serum
  68. Protectin D1 is generated in asthma and dampens airway inflammation and hyperresponsiveness.
    Authors: Levy BD, Kohli P, Gotlinger K, Haworth O, Hong S, Kazani S, Israel E, Haley KJ, Serhan CN
    J. Immunol., 2007;178(1):496-502.
    Species: Mouse
    Sample Types: BALF
  69. CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis.
    Authors: Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA
    Am. J. Respir. Crit. Care Med., 2007;177(3):301-8.
    Species: Mouse
    Sample Types: Plasma
  70. Dectin-1 interaction with Mycobacterium tuberculosis leads to enhanced IL-12p40 production by splenic dendritic cells.
    Authors: Rothfuchs AG, Bafica A, Feng CG, Egen JG, Williams DL, Brown GD, Sher A
    J. Immunol., 2007;179(6):3463-71.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  71. Granulocyte-macrophage colony-stimulating factor prevents diabetes development in NOD mice by inducing tolerogenic dendritic cells that sustain the suppressive function of CD4+CD25+ regulatory T cells.
    Authors: Gaudreau S, Guindi C, Menard M, Besin G, Dupuis G, Amrani A
    J. Immunol., 2007;179(6):3638-47.
  72. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007;245(2):70-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  73. Reversion of immune tolerance in advanced malignancy: modulation of myeloid-derived suppressor cell development by blockade of stem-cell factor function.
    Authors: Pan PY, Wang GX, Yin B, Ozao J, Ku T, Divino CM, Chen SH
    Blood, 2007;111(1):219-28.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  74. Mrp8 and Mrp14 are endogenous activators of Toll-like receptor 4, promoting lethal, endotoxin-induced shock.
    Authors: Vogl T, Tenbrock K, Ludwig S, Leukert N, Ehrhardt C, van Zoelen MA, Nacken W, Foell D, van der Poll T, Sorg C, Roth J
    Nat. Med., 2007;13(9):1042-9.
    Species: Mouse
    Sample Types: Serum
  75. Placental growth factor down-regulates type 1 T helper immune response by modulating the function of dendritic cells.
    Authors: Lin YL, Liang YC, Chiang BL
    J. Leukoc. Biol., 2007;82(6):1473-80.
    Species: Human
    Sample Types: Cell Culture Supernates
  76. Adenosine A2A receptor inactivation increases survival in polymicrobial sepsis.
    Authors: Nemeth ZH, Csoka B, Wilmanski J, Xu D, Lu Q, Ledent C, Deitch EA, Pacher P, Spolarics Z, Hasko G
    J. Immunol., 2006;176(9):5616-26.
    Species: Mouse
    Sample Types: Plasma
  77. CD27 contributes to the early systemic immune response to Mycobacterium tuberculosis infection but does not affect outcome.
    Authors: Wieland CW, Kerver ME, Florquin S, Nolte MA, Borst J, van Lier R, van Oers MH, van der Poll T
    Int. Immunol., 2006;18(11):1531-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  78. Inhalation of ultrafine carbon particles triggers biphasic pro-inflammatory response in the mouse lung.
    Authors: Andre E, Stoeger T, Takenaka S, Bahnweg M, Ritter B, Karg E, Lentner B, Reinhard C, Schulz H, Wjst M
    Eur. Respir. J., 2006;28(2):275-85.
    Species: Mouse
    Sample Types: BALF
  79. MyD88-dependent and -independent murine cytomegalovirus sensing for IFN-alpha release and initiation of immune responses in vivo.
    Authors: Delale T, Paquin A, Asselin-Paturel C, Dalod M, Brizard G, Bates EE, Kastner P, Chan S, Akira S, Vicari A, Biron CA, Trinchieri G, Briere F
    J. Immunol., 2005;175(10):6723-32.
    Species: Mouse
    Sample Types: Serum
  80. Vaccination of apoptotic Fas ligand-expressing tumors decreased antitumor responses by enhanced production of immunosuppressive cytokines.
    Authors: Wada A, Tada Y, Shimozato O, Takiguchi Y, Tatsumi K, Kuriyama T, Tagawa M
    Anticancer Res., 2005;25(1):299-303.
    Species: Mouse
    Sample Types: Tissue Secretion
  81. Endogenous interleukin-12 improves the early antimicrobial host response to murine Escherichia coli peritonitis.
    Authors: Weijer S, Florquin S, van der Poll T
    Shock, 2005;23(1):54-8.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  82. Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
    Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
    Vaccine, 2005;24(9):1470-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  83. Suppression of autoimmune retinal disease by lovastatin does not require Th2 cytokine induction.
    Authors: Gegg ME, Harry R, Hankey D, Zambarakji H, Pryce G, Baker D, Adamson P, Calder V, Greenwood J
    J. Immunol., 2005;174(4):2327-35.
    Species: Rat
    Sample Types: Cell Culture Supernates
  84. Increased and long-term generation of dendritic cells with reduced function from IL-6-deficient bone marrow.
    Authors: Bleier JI, Pillarisetty VG, Shah AB, DeMatteo RP
    J. Immunol., 2004;172(12):7408-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  85. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
    Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
    Comp. Immunol. Microbiol. Infect. Dis., 2004;27(6):393-411.
    Species: Mouse
    Sample Types: Serum
  86. Effects of poly(ADP-ribose) polymerase inhibition on inflammatory cell migration in a murine model of asthma.
    Authors: Virag L, Bai P, Bak I, Pacher P, Mabley JG, Liaudet L, Bakondi E, Gergely P, Kollai M, Szabo C
    Med. Sci. Monit., 2004;10(3):BR77-83.
    Species: Mouse
    Sample Types: BALF
  87. Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
    Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
    J. Immunol., 2004;172(3):1754-62.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  88. Gamma interferon does not enhance clearance of Pseudomonas aeruginosa but does amplify a proinflammatory response in a murine model of postseptic immunosuppression.
    Authors: Murphey ED, Herndon DN, Sherwood ER
    Infect. Immun., 2004;72(12):6892-901.
    Species: Mouse
    Sample Types: Plasma
  89. A protective role of locally administered immunostimulatory CpG oligodeoxynucleotide in a mouse model of genital herpes infection.
    Authors: Harandi AM, Eriksson K, Holmgren J
    J. Virol., 2003;77(2):953-62.
    Species: Mouse
    Sample Types: Serum
  90. Endogenous granulocyte-macrophage colony-stimulating factor overexpression in vivo results in the long-term recruitment of a distinct dendritic cell population with enhanced immunostimulatory function.
    Authors: Miller G, Pillarisetty VG, Shah AB, Lahrs S, Xing Z, DeMatteo RP
    J. Immunol., 2002;169(6):2875-85.
    Species: Mouse
    Sample Types: Serum
  91. Cutting edge: myeloid differentiation factor 88 deficiency improves resistance against sepsis caused by polymicrobial infection.
    Authors: Weighardt H, Kaiser-Moore S, Vabulas RM, Kirschning CJ, Wagner H, Holzmann B
    J. Immunol., 2002;169(6):2823-7.
    Species: Mouse
    Sample Types: Serum
  92. A profibrotic function of IL-12p40 in experimental pulmonary fibrosis.
    Authors: Huaux F, Arras M, Tomasi D, Barbarin V, Delos M, Coutelier JP, Vink A, Phan SH, Renauld JC, Lison D
    J. Immunol., 2002;169(5):2653-61.
    Species: Mouse
    Sample Types: Tissue Homogenates
  93. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
    Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
    Eur. J. Immunol., 2001;31(7):2104-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  94. In vitro and in vivo immunostimulatory potential of bone marrow-derived mast cells on B- and T-lymphocyte activation.
    Authors: Tkaczyk C, Villa I, Peronet R, David B, Chouaib S, Mecheri S
    J. Allergy Clin. Immunol., 2000;105(1):134-42.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  95. Local anticandidal immune responses in a rat model of vaginal infection by and protection against Candida albicans.
    Authors: de Bernardis F, Santoni G, Boccanera M, Spreghini E, Adriani D, Morelli L, Cassone A
    Infect. Immun., 2000;68(6):3297-304.
    Species: Rat
    Sample Types: Vaginal Fluid

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Mouse IL-12 p70 Quantikine ELISA Kit
By Anonymous on 11/02/2018
Application: Sample Tested: Serum

Mouse IL-12 p70 Quantikine ELISA Kit
By Anonymous on 10/07/2018
Application: Sample Tested: EDTA Plasma