Mouse IL-12 R beta 1 PE-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-12 R beta 1 in direct ELISAs and Western blots. In these formats, approximately 20% cross‑reactivity with recombinant human IL-12 R beta 1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12 R beta 1
    Val32-Glu561
    Accession # Q60837
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of IL‑12 R beta 1 in D10.G4.1 Mouse Cell Line by Flow Cytometry. D10.G4.1 mouse helper T cell line was stained with Goat Anti-Mouse IL‑12 R beta 1 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1998P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12 R beta 1

IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G-CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16%-21% aa sequence identity with the ECDs of mouse gp130, LIF R, G-CSF R, and IL-23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL-23 R (4-6). IL-12 R beta 2 and IL-23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8-10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).

  • References:
    1. Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
    2. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
    3. Chua, A.O. et al. (1995) J. Immunol. 155:4286.
    4. Parham, C. et al. (2002) J. Immunol. 168:5699.
    5. Wu, C. et al. (1997) J. Immunol. 159:1658.
    6. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
    7. Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
    8. Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
    9. Airoldi, I. et al. (2000) J. Immunol. 165:6880.
    10. Li, J. et al. (2003) J. Neurol. Sci. 215:95.
    11. Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
    12. Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
    13. Shimozato, O. et al. (2006) Immunology 117:22.
    14. Russell, T.D. et al. (2003) J. Immunol. 171:6866.
  • Long Name:
    Interleukin 12 Receptor beta 1
  • Entrez Gene IDs:
    3594 (Human); 16161 (Mouse)
  • Alternate Names:
    CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454
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Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 8  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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