Mouse IL‑12 R beta 1 Biotinylated Antibody

Name: Mouse IL‑12 R beta 1 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1998
Price: 499 USD
Availability: InStock

R&D Systems | Catalog # BAF1998

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Citations (4)

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Product Specifications
Scientific Data
Applications
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Calculators
Background
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all IL-12 R beta 1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12 R beta 1
Val32-Glu561
Accession # Q60837

Specificity

Detects mouse IL-12 R beta 1 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human IL-12 R beta 1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse IL‑12 R beta 1 Biotinylated Antibody

Detection of IL-12 R beta 1 by Western Blot

Murine p40 with D36K/W37K/T38K substitutions interacts with murine p19 but not with murine IL-12R beta 1.A, structure of hIL-23 extracted from the structure of the IL-23:IL-23R complex structure (PDB 5mzv). A flexible linker sequence connecting p40 and p19 is indicated. B, Western blot analysis of secreted murine HIL-23, mp19 and mp40 variants from transfected CHO-K1 cells. The position of mp19 and mp40 variants is indicated by arrows. C, cellular proliferation of Ba/F3-gp130-mIL-12R beta 1-mIL-23R cells. The cells were cultured for 3 days in the presence of 10 ng/ml HIL-6 or with the indicated cytokines (10% conditioned cell culture supernatant of transfected CHO-K1 cells). Parental Ba/F3-gp130 cells were used as controls. The results of one representative experiment of three are shown. Error bars represent S.D. for technical replicates. Statistical analysis used a one-way ANOVA, followed by Bonferroni correction (n = 3), ∗∗∗p ≤ 0.001. D, analysis of STAT3 and ERK1/2 activation. Ba/F3-gp130-mIL-12R beta 1-mIL-23R cells were washed, starved, and stimulated with the indicated cytokines (10% conditioned cell culture supernatant of transfected CHO-K1 cells) for 30 min. Cellular lysates were prepared, and equal amounts of total protein (50 μg/lane) were loaded on SDS-PAA gels, followed by immunoblotting using specific antibodies for phospho-STAT3, STAT3, phospho-ERK1/2, and ERK1/2. Western blotting data show results of one representative experiment of two. E, co-IP of FLAG-tagged murine mp19 coexpressed with either mp40 or mp40D36K/W37K/T38K and full-length mIL-12R beta 1 or mIL-23R. The position of mIL-23R and mIL-12R beta 1 is indicated by arrows. One of two independent experiments is shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34637790), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse IL‑12 R beta 1 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse IL‑12 R beta 1 (Catalog # 1998-B1)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-12 R beta 1

IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G‑CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16%‑21% aa sequence identity with the ECDs of mouse gp130, LIF R, G‑CSF R, and IL‑23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL‑23 R (4‑6). IL‑12 R beta 2 and IL‑23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8‑10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).