Mouse IL-12 R beta 1 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1998
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Mouse IL-12 R beta 1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-12 R beta 1 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human IL-12 R beta 1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-12 R beta 1
Val32-Glu561
Accession # Q60837
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑12 R beta 1 (Catalog # 1998-B1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 1

IL-12 R beta 1 is a 100 kDa type I transmembrane protein that belongs to the gp130/G‑CSF R family of cytokine receptors. IL-12 R beta 1 is a common subunit of both the IL‑12 and IL-23 receptor complexes which play distinct but related roles in T cell mediated inflammatory reactions (1, 2). Mature mouse IL-12 R beta 1 contains a 546 amino acid (aa) extracellular domain (ECD) with five fibronectin type III repeats, and a 147 aa cytoplasmic domain (3). Within the ECD, mouse IL-12 R beta 1 shares 85% and 52% aa sequence identity with rat and human IL-12 R beta 1, respectively. It shares 16%‑21% aa sequence identity with the ECDs of mouse gp130, LIF R, G‑CSF R, and IL‑23 R. IL-12 and IL-23 are disulfide linked heterodimeric cytokines that share a common p40 subunit (1, 2). IL-12 R beta 1 interacts with p40 at low affinity but does not transmit signals (3). Increased ligand binding affinity and signaling capacity are gained by association of IL-12 R beta 1 with either IL-12 R beta 2 or IL‑23 R (4‑6). IL‑12 R beta 2 and IL‑23 R are the signal transducing components of these receptor complexes (4, 7). IL-12 R beta 1 is expressed on activated T cells, NK cells, B cells, macrophages, and microglia (8‑10). IL-12 induced signaling promotes the development of naïve T cells into IFN-beta producing Th1 cells (11). IL-23 contributes to chronic inflammation by inducing the production of IL-17 by memory T cells (12). Naturally occurring homodimers of p40 can function as antagonists of IL-12 and IL‑23 and can also induce macrophage chemotaxis in the absence of IL-12 R beta 2 (13, 14).

References
  1. Becker, C. et al. (2005) Inflamm. Bowel Dis. 11:755.
  2. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  3. Chua, A.O. et al. (1995) J. Immunol. 155:4286.
  4. Parham, C. et al. (2002) J. Immunol. 168:5699.
  5. Wu, C. et al. (1997) J. Immunol. 159:1658.
  6. Zou, J. et al. (1997) J. Biol. Chem. 272:6073.
  7. Presky, D.H. et al. (1996) Proc. Natl. Acad. Sci. 93:14002.
  8. Wu, C. et al. (1997) Eur. J. Immunol. 27:147.
  9. Airoldi, I. et al. (2000) J. Immunol. 165:6880.
  10. Li, J. et al. (2003) J. Neurol. Sci. 215:95.
  11. Schmitt, E. et al. (1994) Eur. J. Immunol. 24:793.
  12. Yen, D. et al. (2006) J. Clin. Invest. 116:1310.
  13. Shimozato, O. et al. (2006) Immunology 117:22.
  14. Russell, T.D. et al. (2003) J. Immunol. 171:6866.
Long Name
Interleukin 12 Receptor beta 1
Entrez Gene IDs
3594 (Human); 16161 (Mouse)
Alternate Names
CD212 antigen; CD212; IL-12 R beta 1; IL-12 receptor beta component; IL-12 receptor subunit beta-1; IL12R beta 1; IL-12R subunit beta-1; IL12R; IL12RB; IL12RB1; IL-12Rb1; IL-12R-BETA1; IL-12R-beta-1; interleukin 12 receptor, beta 1; interleukin-12 receptor beta-1 chain; interleukin-12 receptor subunit beta-1; MGC34454

Product Datasheets

You must select a language.

x

Citation for Mouse IL-12 R beta 1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Tryptophan (W) at position 37 of murine IL-12/IL-23 p40 is mandatory for binding to IL-12Rbeta1 and subsequent signal transduction
    Authors: J Georgy, Y Arlt, JM Moll, M Ouzin, HT Weitz, L Gremer, D Willbold, J Grötzinger, F Thives-Kur, J Scheller, DM Floss
    The Journal of Biological Chemistry, 2021-10-09;0(0):101295.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse IL-12 R beta 1 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse IL-12 R beta 1 Biotinylated Antibody and earn rewards!

Have you used Mouse IL-12 R beta 1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review