Mouse IL-17 Quantikine HS ELISA Kit

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Catalog # Availability Size / Price Qty
MHS170
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Mouse IL-17 Quantikine HS ELISA Standard Curve
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Mouse IL-17 Quantikine HS ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
0.214 pg/mL
Assay Range
0.8 - 50 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine™ HS Mouse IL-17 Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse IL-17 levels in serum and plasma. It contains E. coli-expressed recombinant mouse IL-17 and antibodies raised against the recombinant protein. Results obtained using natural IL-17 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-17.

Recovery

The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 90 78-96
Heparin Plasma (n=4) 89 80-97
Serum (n=4) 89 75-98

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-17 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Mouse IL-17 Quantikine HS Linearity

Scientific Data

Mouse IL-17 Quantikine HS ELISA Standard Curve

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:
Interleukin 17
Entrez Gene IDs:
3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)
Alternate Names:
CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A

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