Mouse IL-17 Quantikine HS ELISA Kit

R&D Systems | Catalog # MHS170

R&D Systems
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Key Product Details

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity

0.214 pg/mL

Assay Range

0.781-50 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
View all IL-17/IL-17A ELISA Kits »

Product Summary for Mouse IL-17 Quantikine HS ELISA Kit

The Quantikine™ HS Mouse IL-17 Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse IL-17 levels in serum and plasma. It contains E. coli-expressed recombinant mouse IL-17 and antibodies raised against the recombinant protein. Results obtained using natural IL-17 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-17.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450 or 490nm (TMB or AP Substrate)

Conjugate

HRP

Species

Mouse

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 6.82 16.9 31.9 6.92 16.6 31.4
Standard Deviation 0.239 0.429 0.836 0.349 0.814 1.64
CV% 3.5 2.5 2.6 5.1 4.9 5.2

Recovery for Mouse IL-17 Quantikine HS ELISA Kit

The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 90 78-96
Heparin Plasma (n=4) 89 80-97
Serum (n=4) 89 75-98

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-17 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Mouse IL-17 Quantikine HS ELISA Kit

Mouse IL-17 Quantikine HS ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17/IL-17A

IL-17/IL-17A is a homodimeric cytokine that is secreted by IL-23 activated Th17 and Tc17 cells. It is upregulated during inflammation and contributes to disease severity by enhancing neutrophil infiltration and the production of pro-inflammatory chemokines. IL-17A can additionally form bioactive heterodimers with IL-17F. IL-17A and the IL-17A/F heterodimer signal through widely expressed receptor complexes containing IL-17 RA and IL-17 RC.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for Mouse IL-17 Quantikine HS ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse IL-17 Quantikine HS ELISA Kit

For research use only

Citations for Mouse IL-17 Quantikine HS ELISA Kit

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  • LPS induced inflammation in mice
    Name: Anonymous
    Sample Tested: Lung tissue
    Species: Mouse
    Verified Customer | Posted 03/28/2026
    lps induced inflammation in mice
    IL-17 used in lung homogenate
    Mouse IL-17 Quantikine HS ELISA Kit MHS170

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