Mouse IL-17RC APC‑conjugated Antibody

Detection of IL‑17 RC in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse IL-17 RC APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2270A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of Mouse IL-17RC by Flow Cytometry

IL-17 promotes STAT-3 phosphorylation in Vk*MYC plasma cells. a Th17 polarization of OT-II splenocytes cultured for 7 days with BM serum obtained from WT, Early-MM and Late-MM Vk*MYC mice, and assessed for intracellular cytokine release by flow cytometry. None and Cytokines refer to the culture condition with or without IL-6, TGF-beta 1, anti-IL-4, and anti-IFN-gamma antibodies, respectively. (None n = 3, Cytokine n = 3, WT n = 6, Vk*MYC Early n = 11, Vk*MYC Late n = 11). Mean ± SD of three independent experiments. Unpaired t test: *P < 0.05; **P < 0.01; ***P < 0.001. b Plasma cells were also stained with anti-IL-17RA and anti-IL-17RC antibodies (blue and red line respectively) and analyzed by flow-cytometry; FMO (Fluorescence Minus One) sample was not stained for IL-17R (gray histogram). c, d Representative histograms and e quantification of Vk*MYC PCs cultured in the presence of either one of the following stimuli: saturating amounts of IL6 (light blue line) or IL-17 (dark blue line), or BM sera from Early- (red line) or Late-MM (black dotted line), or BM sera from Early-MM and anti-IL17 antibodies (purple line). After culture, plasma cells were analyzed by flow-cytometry for STAT3 phosphorylation (pSTAT3). (IL-6 n = 5, IL-17A n = 5, Vk*MYC Early n = 8, Vk*MYC Early +  alpha IL-17A n = 8, Vk*MYC Late n = 8). Mean ± SD of triplicate independent determinations. Unpaired t test: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30510245), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse IL-17RC by Flow Cytometry

An IL-17-eosinophil axis in the BM of Vk*MYC mice favors disease progression. a Frequency of BM eosinophils (i.e., CD11b+Ly6CintMHC-II−Ly6G−SSChi or CD11b+Siglec-F+ cells) in Vk*MYC IL-17WT and Vk*MYC IL-17KO mice and age- and sex-matched WT littermates. Each dot represents an individual mouse. Mean ± SD of five independent experiments. Unpaired t test: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. b Representative dot plot of IL-6+ cells (gated on CD11b+Siglec-F+ cells) in the BM. c Percentage of IL-6+ cells gated on CD11b+Siglec-F+ cells. Mean ± SD of five independent experiments. Unpaired t test. d Mean fluorescence intensity (MFI) of IL-6 within Siglec-F+CD11b+ cells. Mean ± SD of five independent experiments. Unpaired t test. e BM derived eosinophils were also stained with anti-IL-17RA and anti-IL-17RC antibodies (blue and red line respectively) and analyzed by flow-cytometry; FMO (Fluorescence Minus One) sample was not stained for IL-17R (gray histogram). f Representative histograms of IL-6 and TNF-alpha production by eosinophils after IL-17A stimulation (red line). FMO samples were not stained for IL-6 or TNF-alpha. g Representative histograms of IL-6 and TNF-alpha production by eosinophils after MCP-3 stimulation (blue line). FMO samples were not stained for IL-6 or TNF-alpha. h IL-6 levels (MFI normalized on FMO sample) in eosinophils cultured alone (None; n = 4), or in the presence of WT (n = 4) or Early-MM (n = 8) or Late-MM (n = 5) BM serum. Mean ± SD of aggregated data from three independent experiments. Unpaired t test. i IL-6 levels (MFI normalized on Early-MM sample) in eosinophils cultured alone (None; n = 5), or in the presence of Early-MM with or without the addition of anti-CCR3 (n = 5) or anti-IL-17A (n = 5). Mean ± SD of aggregated data from three independent experiments. Paired t test. a, c, d Specific n values of biologically independent mice are shown Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30510245), licensed under a CC-BY license. Not internally tested by R&D Systems.