Mouse IL-17RC Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2270
Detection of IL‑17 RC in RAW 264.7 Mouse Cell Line by Flow Cytometry.
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Mouse IL-17RC Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑17 RC in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) IL‑17 RC is observed and less than 2% cross‑reactivity with rhIL‑17 R, recombinant mouse (rm) IL‑17 RD and rmIL‑17B R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL‑17 RC
Leu21-Trp465
Accession # AAH04759
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑17 RC (Catalog # 2270-ML)
Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-17 RC antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of IL‑17 RC in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse IL-17 RC Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2270, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RC

IL-17 receptor C (IL-17 RC; also known as IL-17 RL) is an 85‑110 kDa member of the IL-17 receptor family. This is one of five families, termed IL-17 RA, B, C, D and E, that comprise the cytokine receptor superfamily (1‑6). Not all receptors appear to bind known members of the IL-17 cytokine family. To date, IL-17 RA is reported to bind IL-17A, while IL-17 RB is reported to bind IL-17B and IL-17E (2, 4). Mouse IL-17 RC is a type I transmembrane glycoprotein that is expressed on a variety of nonhematopoietic cell types. Full-length IL-17 RC is synthesized as a 674 amino acid (aa) precursor. It contains a 21 aa signal sequence, a 419 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 213 aa cytoplasmic region. There are multiple potential N‑linked glycosylation sites in the ECD and potential phosphorylation sites in the cytoplasmic tail. Four mouse variants have been identified that have been designated mIL-17 RC (7). The isoform expressed here as an R&D product is an unusual 567 aa form (8). Its precursor contains a 20 aa signal sequence, a 444 aa extracellular region, a 20 aa transmembrane segment and an 83 aa cytoplasmic tail. When compared to the full length mouse IL-17 RC form, this expressed isoform’s extracellular region shows absolute aa identity, save for an additional 24 aa insert. In the cytoplasmic region, it is highly divergent and shows virtually no aa identity (8‑9). The extracellular region of mouse IL-17 RC shows about 70% aa identity to the equivalent region in human IL-17 RC isoform # 3. IL-17 RC is the cognate receptor for IL-17F (7). In humans, IL-17 RC binds IL-17A with similar affinity, and with IL-17 RA, it forms a definitive receptor for both IL-17A and IL-17F (7). The stoichiometry is unclear; it may form a heterodimer with IL-17 RA, or a heterotrimer with a preexisting IL-17 RA homodimer (4, 7, 10, 11). The heteromeric nature of the receptor may be important given that the predominant form of the IL-17 cytokine is now considered to be an IL-17A:IL-17F heterodimer (4).

References
  1. Gaffen, S.L. et al. (2006) Vitam. Horm. 74:255.
  2. Weaver, C.T. et al. (2007) Annu. Rev. Immunol. 25:821.
  3. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155.
  4. Shen, F. and S.L. Gaffen (2008) Cytokine 41:92.
  5. You, Z. et al. (2006) Cancer Res. 66:175.
  6. You, Z. et al. (2007) Neoplasia 9:464.
  7. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  8. GenBank Accession # AAH04759.
  9. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  10. Toy, D. et al. (2006) J. Immunol. 177:36.
  11. Haudenschild, D.R. et al. (2006) Prostate 66:1268.
Long Name
Interleukin 17 Receptor C, Transcript Variant 3
Entrez Gene IDs
84818 (Human); 171095 (Mouse)
Alternate Names
FLJ95963; FLJ96005; IL-17 RC; IL-17 receptor C; IL-17 RL; IL17F receptor; IL17RC; IL-17RC; IL17Rhom; IL17RL; IL-17RL; IL17-RL; interleukin 17 receptor C; interleukin-17 receptor C; Interleukin-17 receptor homolog; Interleukin-17 receptor-like protein; MGC10763; zcytoR14

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Citation for Mouse IL-17RC Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. IL-17F induces inflammation, dysfunction and cell death in mouse islets
    Authors: T Catterall, S Fynch, TWH Kay, HE Thomas, APR Sutherland
    Sci Rep, 2020-08-04;10(1):13077.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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