Mouse IL-18/IL-1F4 Antibody (74)

Detection of IL-18/IL-1F4 by Western Blot

Parkin Restricts Mitochondrial Damage, mtROS Overproduction, and mtROS-Induced Inflammasome Activation during Viral Infection(A) Immunoblot analysis of p62, LC3II, and Park2 in cytosolic and mitochondrial subcellular fractions from Pam3CSK4-primed WT and Park2−/− BMDMs infected with VSV at MOI 5 or HSV-1 at MOI 10 for 5 h.(B) Microscopic analysis of mitochondrial membrane potential of cells stimulated with VSV or HSV-1 at MOI 1 by TMRM (tetramethylrhodamine, methyl ester) fluorescence. UI, uninfected. Scale bar, 100 μm.(C) Flow cytometry analysis of the levels of mitochondria-associated ROS (mtROS) labeled by MitoSOX in Pam3CSK4-primed WT and Park2−/− BMDMs untreated or treated with 50 nM Mito-Q (mitochondrial-specific antioxidant) followed by VSV or HSV-1 infection at MOI 5 for 12 h.(D) ELISA analysis of 8-OH-dG in the cytosol of Pam3CSK4-primed WT and Park2−/− BMDMs treated with VSV and HSV-1 at MOI 10 for 5 h.(E) ELISA analysis of IL-1 beta, IL-18, and TNF-alpha in the supernatants of Pam3CSK4-primed WT and Park2−/− BMDMs untreated or treated with 50 nM Mito-Q and then infected with VSV at MOI 1 or HSV-1 at MOI 2 for 24 h (n = 3).(F) Immunoblot analysis of cleaved IL-1 beta and caspase-1 in culture supernatants (SN) of Pam3CSK4-primed WT and Park2−/− BMDMs treated with 50 nM Mito-Q and then infected with VSV at MOI 5 or HSV-1 at MOI 10 for 24 h.(G) LDH assay of Pam3CSK4-primed WT and Park2−/− BMDMs treated as indicated in (E).Data are pooled from three (D, E, and G) independent experiments or are representative of two independent experiments (A, B, C, and F). Error bars show means ± SEM. *p < 0.05, ***p < 0.001. NS, not significant. Unpaired t test or two-way ANOVA with Sidak's multiple comparisons test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31229895), licensed under a CC-BY license. Not internally tested by R&D Systems.