Detection of IL‑27 R alpha /WSX‑1/TCCR in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse IL‑27 R alpha /WSX‑1/TCCR PE‑conjugated Monoclonal Antibody (Catalog # FAB21091P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-27 R alpha/WSX-1/TCCR
IL-27 R alpha, also known as WSX-1 and TCCR, is a 85‑95 kDa member of the type I, group 2 cytokine receptor family (1-6). Mature IL-27 R alpha is a type I transmembrane glycoprotein that contains a 486 amino acid (aa) extracellular region, a 21 aa transmembrane segment and a 92 aa cytoplasmic domain. Consistent with type I cytokine receptors, the extracellular region contains four positionally conserved cysteine residues, a WSxWS motif (for receptor folding and ligand binding), and three fibronectin type III repeats. The intracellular domain contains a “box-1” motif that may be involved with Janus kinases (3). In mouse, a soluble 33 kDa splice form that shows a 20 aa substitution for aa 251-623 has been identified (7). The mouse IL-27 R alpha extracellular region shares 63% amino acid identity with the human IL-27 R alpha extracellular domain (2, 3). IL-27 R alpha is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells (1, 2, 4, 8). Typical of other class I cytokine receptor chains, the ligand binding IL-27 R alpha molecule is known to heterodimerize with a signal-transducing subunit (gp130) to form a functional IL-27 receptor (9, 10). In addition, IL-27 R alpha is reported to complex with CNTFR alpha and gp130 form a humanin receptor on neurons (7, 11), and to complex with gp130 and IL-6 R to form a receptor for a p28:CLF heterodimeric cytokine on lymphocytes (12). Studies using IL-27 R alpha /WSX-1-/- mice reveal that IL-27 has the ability to suppress T cell activity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity (4, 13, 14). In particular, IL-27 is known to act on naïve T cells, blocking their differentiation into a Th17 phenotype. Notably, cells committed to a Th17 phenotype, although they express a functional IL-27 receptor, are unresponsive to the effects of IL-27 (15). Activated T cells that are CD4+ and CD8+, and which express the IL-27 receptor, can be induced by IL‑27 to form a double-positive CD25+ FoxP3- IFN-gamma plus IL-10 secreting phenotype that both promotes and suppresses the inflammatory response (16).
Villarino, A.V. et al. (2004) J. Immunol. 173:715.
Chen, Q. et al. (2000) Nature 407:916.
Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82.
Artis, D. et al. (2004) J. Immunol. 173:5626.
Yoshida, H. and Y. Miyazaki (2008) Int. J. Biochem. Cell Biol. 40:2379.
Yoshida, H. and M. Yoshiyuki (2008) Immunol. Rev. 226:234.
Hashimoto, Y. et al. (2009) Biochem. Biophys. Res. Commun. 389:95
Holscher, C. et al. (2005) J. Immunol. 174:3534.
Pflanz, S. et al. (2004) J. Immunol. 172:2225.
Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
Hashimoto, Y. et al. (2009) Mol. Biol. Cell 20:2864.
Crabe, S. et al. (2009) J. Immunol. 183:7692.
Villarino, A. et al. (2003) J. Immunol. 170:645.
Hamano., S. et al. (2003) Immunity 19:657.
El-behi, M. et al. (2009) J. Immunol. 183:4957.
Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
Interleukin-27 Receptor Subunit alpha
Entrez Gene IDs:
9466 (Human); 50931 (Mouse)
class I cytokine receptor; CRL1IL-27R; Cytokine receptor-like 1; IL-27 R alpha; IL27R alpha; IL27R; IL27RA; IL-27Ra; IL-27R-alpha; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; TCCR; TCCRIL-27 receptor subunit alpha; T-cell cytokine receptor type 1; Type I T-cell cytokine receptor; WSX-1; WSX1IL-27R subunit alpha; zcytor1
Have you used Mouse IL-27 R alpha /WSX-1/TCCR PE-conjugated Antibody?
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.