Mouse IL-27 R alpha /WSX-1/TCCR Fluorescein-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects mouse IL-27 R alpha /WSX‑1/TCCR in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse gp130 or recombinant human IL-27 R alpha is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 263503
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-27 R alpha /WSX‑1/TCCR
    Accession # O70394
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑27 R alpha /WSX‑1/TCCR in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse IL‑27 R alpha /WSX‑1/TCCR Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB21091F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-27 R alpha/WSX-1/TCCR

IL‑27 R alpha (also known as WSX‑1 and TCCR) is a 85‑95 kDa member of the type I, group 2 cytokine receptor family (1‑6). Mature IL‑27 R alpha is a type I transmembrane glycoprotein that contains a 486 amino acid (aa) extracellular region, a 21 aa transmembrane segment and a 92 aa cytoplasmic domain. Consistent with type I cytokine receptors, the extracellular region contains four positionally conserved cysteine residues, a WSxWS motif (for receptor folding and ligand binding), and three fibronectin type III repeats. The intracellular domain contains a “box‑1” motif that may be involved with Janus kinases (3). In mouse, a soluble 33 kDa splice form that shows a 20 aa substitution for aa 251‑623 has been identified (7). The mouse IL‑27 R alpha extracellular region shares 63% amino acid identity with the human IL‑27 R alpha extracellular domain (2, 3). IL‑27 R alpha is expressed in mast cells, endothelial cells, NK cells, macrophages, monocytes, B cells, dendritic cells, and naïve T cells (1, 2, 4, 8). Typical of other class I cytokine receptor chains, the ligand binding IL‑27 R alpha molecule is known to heterodimerize with a signal‑transducing subunit (gp130) to form a functional IL‑27 receptor (9, 10). In addition, IL‑27 R alpha is reported to complex with CNTFR alpha and gp130 form a humanin receptor on neurons (7, 11), and to complex with gp130 and IL‑6 R to form a receptor for a p28:CLF heterodimeric cytokine on lymphocytes (12). Studies using IL‑27 R alpha /WSX‑1-/- mice reveal that IL‑27 has the ability to suppress T cell activity during infection, and to mediate an inhibition of both type 1 and type 2 T cell immunity (4, 13, 14). In particular, IL‑27 is known to act on naïve T cells, blocking their differentiation into a Th17 phenotype. Notably, cells committed to a Th17 phenotype, although they express a functional IL‑27 receptor, are unresponsive to the effects of IL‑27 (15). Activated T cells that are CD4+ and CD8+, and which express the IL‑27 receptor, can be induced by IL‑27 to form a double‑positive CD25+ FoxP3- IFN‑ gamma plus IL‑10 secreting phenotype that both promotes and suppresses the inflammatory response (16).

  • References:
    1. Villarino, A.V. et al. (2004) J. Immunol. 173:715.
    2. Chen, Q. et al. (2000) Nature 407:916.
    3. Sprecher, C.A. et al. (1998) Biochem. Biophys. Res. Commun. 246:82.
    4. Artis, D. et al. (2004) J. Immunol. 173:5626.
    5. Yoshida, H. and Y. Miyazaki (2008) Int. J. Biochem. Cell Biol. 40:2379.
    6. Yoshida, H. and M. Yoshiyuki (2008) Immunol. Rev. 226:234.
    7. Hashimoto, Y. et al. (2009) Biochem. Biophys. Res. Commun. 389:95
    8. Holscher, C. et al. (2005) J. Immunol. 174:3534.
    9. Pflanz, S. et al. (2004) J. Immunol. 172:2225.
    10. Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
    11. Hashimoto, Y. et al. (2009) Mol. Biol. Cell 20:2864.
    12. Crabe, S. et al. (2009) J. Immunol. 183:7692.
    13. Villarino, A. et al. (2003) J. Immunol. 170:645.
    14. Hamano., S. et al. (2003) Immunity 19:657.
    15. El-behi, M. et al. (2009) J. Immunol. 183:4957.
    16. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  • Long Name:
    Interleukin-27 Receptor Subunit alpha
  • Entrez Gene IDs:
    9466 (Human); 50931 (Mouse)
  • Alternate Names:
    class I cytokine receptor; CRL1IL-27R; Cytokine receptor-like 1; IL-27 R alpha; IL27R alpha; IL27R; IL27RA; IL-27Ra; IL-27R-alpha; interleukin 27 receptor, alpha; interleukin-27 receptor subunit alpha; TCCR; TCCRIL-27 receptor subunit alpha; T-cell cytokine receptor type 1; Type I T-cell cytokine receptor; WSX-1; WSX1IL-27R subunit alpha; zcytor1
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. IL-27 acts on DCs to suppress the T cell response and autoimmunity by inducing expression of the immunoregulatory molecule CD39.
    Authors: Mascanfroni, Ivan D, Yeste, Ada, Vieira, Silvio M, Burns, Evan J, Patel, Bonny, Sloma, Ido, Wu, Yan, Mayo, Lior, Ben-Hamo, Rotem, Efroni, Sol, Kuchroo, Vijay K, Robson, Simon C, Quintana, Francisc
    Nat Immunol, 2013;14(10):1054-63.
    Species: Mouse
    Sample Type: whole cells
    Application: Flow
Isotype Controls
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Rat IgG2B Fluorescein-conjugated Antibody

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Flow Cytometry Staining Buffer (1X)

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Flow Cytometry Mouse Lyse Buffer (10X)

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Flow Cytometry Human Lyse Buffer (10X)

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