Mouse IL-3 R alpha /CD123 PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse IL-3 R alpha in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-3 R alpha  and recombinant mouse IL-3 R beta is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 151231
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL-3 R alpha
    Accession # P26952
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑3 R alpha /CD123 in DA3 Mouse Cell Line by Flow Cytometry. DA3 mouse myeloma cell line was stained with Rat Anti-Mouse IL‑3 R alpha /CD123 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB983C, filled histogram) or isotype control antibody (Catalog # IC006C, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-3 R alpha/CD123

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha  subunit and the beta  subunit. The alpha  subunit alone binds IL-3 with low affinity. The beta  subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. The mouse IL-3 R alpha, also called SUT-1, will form complexes with either mouse IL-3 R beta protein (3). Both the alpha and the beta  subunits are members of the cytokine receptor superfamily.

  • References:
    1. Itoh, N. et al. (1990) Science 247:324.
    2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
    3. Hara, T. and A. Miyajima (1992) EMBO J. 11:1875.
    4. Schrader, J.W. (2001) Cytokine Reference, Oppenheim, J.J. and M. Feldmann, eds, Academic Press, New York, p. 1899.
  • Long Name:
    Interleukin 3 Receptor alpha
  • Entrez Gene IDs:
    3563 (Human); 16188 (Mouse)
  • Alternate Names:
    CD123 antigen; CD123; hIL3Ra; hIL-3Ra; IL-3 receptor subunit alpha; IL3R alpha; IL-3R subunit alpha; IL3R; IL3RA; IL-3Ra; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha; MGC34174
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A PerCP‑conjugated Control Antibody

Ctrl IC006C
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse IL-3 R alpha /CD123 PerCP-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee


Customer Information