Mouse IL-38/IL-1F10 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7774V-100UG
Detection of IL‑38/IL‑1F10 in RAW 264.7 Mouse Cell Line by Flow Cytometry
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Mouse IL-38/IL-1F10 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-38/IL-1F10 in ELISA and Western Blot.
Source
Monoclonal Rat IgG2A Clone # 798036
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-38/IL-1F10
Met1-Arg152
Accession # Q8R459
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of IL‑38/IL‑1F10 in RAW 264.7 Mouse Cell Line by Flow Cytometry View Larger

Detection of IL‑38/IL‑1F10 in RAW 264.7 Mouse Cell Line by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL‑38/IL‑1F10 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB7774V, filled histogram) or isotype control antibody (Catalog # IC006V, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Membrane-associated Proteins.

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Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-38/IL-1F10

IL-1F10, also known as IL-1HY2, is a secreted protein that shares sequence and structural similarity with IL-1 receptor antagonist and other IL-1 family members. It is expressed in basal epithelia of the skin and in proliferating germinal center CD20+ B cells. IL-1F10 binds soluble IL-1RI in vitro and may participate in regulation of immune responses. Mouse IL-1F10 shares 82% and 93% aa identity with human and rat IL-1F10, respectively. IL-1F10 is predicted to be 17 kDa.

Long Name
Interleukin 1 Family Member 10
Entrez Gene IDs
84639 (Human); 215274 (Mouse)
Alternate Names
FIL1- theta; FKSG75; IL1F10; IL-1HY2; IL38; IL-38; interleukin 1 family, member 10 (theta); UNQ6119/PRO20041

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