Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 798036
View all IL-38/IL-1F10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-38/IL-1F10
Met1-Arg152
Accession # Q8R459

Specificity

Detects mouse IL-38/IL-1F10 in ELISA and Western Blot.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse IL‑38/IL‑1F10 Antibody

Detection of Mouse IL-38/IL-1F10 antibody by Western Blot.

Detection of Mouse IL‑38/IL‑1F10 by Western Blot.

Western blot shows lysates of mouse skin tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-38/IL-1F10 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of IL-38/IL-1F10 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

Detection of IL‑38/IL‑1F10 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB7774, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Mouse IL‑38/IL‑1F10 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Western Blot

2 µg/mL
Sample: Mouse skin tissue

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-38/IL-1F10

IL-1F10, also known as IL-1HY2, is a secreted protein that shares sequence and structural similarity with IL-1 receptor antagonist and other IL-1 family members. It is expressed in basal epithelia of the skin and in proliferating germinal center CD20+ B cells. IL-1F10 binds soluble IL-1RI in vitro and may participate in regulation of immune responses. Mouse IL-1F10 shares 82% and 93% aa identity with human and rat IL-1F10, respectively. IL-1F10 is predicted to be 17 kDa.

Long Name

Interleukin 1 Family Member 10

Alternate Names

FIL1- theta, FKSG75, IL-1HY2, IL1F10, IL38

Entrez Gene IDs

84639 (Human); 215274 (Mouse); 102125744 (Cynomolgus Monkey)

Gene Symbol

IL1F10

UniProt

Q8R459

Additional IL-38/IL-1F10 Products

Product Documents for Mouse IL‑38/IL‑1F10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL‑38/IL‑1F10 Antibody

For research use only

Related Research Areas

IL-1 Family

Citations for Mouse IL‑38/IL‑1F10 Antibody

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