Mouse Integrin  alpha 9 PE-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Integrin alpha 9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human Integrin alpha 1, alpha 8, alpha 9, alpha L, recombinant mouse Integrin 3, 4, 5, E, and M is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Integin alpha 9
    Try31-Val979
    Accession # NP_598482
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Integrin  alpha 9 in D3 Mouse Cell Line by Flow Cytometry. D3 mouse embryonic stem cell line was stained with Goat Anti-Mouse Integrin  alpha 9 PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3827P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 9
Integrin alpha 9 is a 150 kDa type I transmembrane glycoprotein that is widely expressed and is found on smooth muscle, keratinocytes, skeletal muscle and hepatocytes. It principally associates with the beta 1 Integrin. alpha 9 beta 1 binds to VEGF-C, VEGF-D, and osteopontin. The 951 amino acid (aa) extracellular domain of mouse Integrin alpha 9 contains three beta -propellor repeats and multiple PheGly-GlyAlaPro repeats. A potential proteolytic cleavage site in human alpha 9 ECD is absolutely conserved in mouse alpha 9 ECD (Arg566-Val567). The ECD of mouse Integrin alpha 9 shares 95% and 89% aa sequence identity to rat and human Integrin alpha 9, respectively.
  • Entrez Gene IDs:
    3680 (Human); 104099 (Mouse); 685004 (Rat)
  • Alternate Names:
    ALPHA-RLC; Integrin alpha 9; integrin alpha-9; Integrin alpha-RLC; integrin, alpha 9; ITGA4L; ITGA9; RLC; RLC-alpha
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Isotype Controls
Description Application Cat# Citations Images  

Goat IgG PE-conjugated Antibody

Ctrl IC108P 8  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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