Mouse LOX-1/OLR1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1564P

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of LOX‑1/OLR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry.
1 Image
Product Details
Citations (5)
FAQs
Supplemental Products
Reviews

Mouse LOX-1/OLR1 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LOX-1 in direct ELISAs and Western blots. In these formats, this antibody does not cross-react with recombinant human LOX‑1.
Source
Monoclonal Rat IgG2A Clone # 214012
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse LOX-1
Arg60-Ile363
Accession # AAG44998
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LOX-1/OLR1 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of LOX‑1/OLR1 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse LOX-1/OLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB1564P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LOX-1/OLR1

Lectin-like oxidized low-density-lipoprotein receptor-1 (LOX-1), also known as oxidized low-density-lipoprotein receptor-1 (OLR-1), is a type II transmembrane receptor belonging to the C-type lectin family (1). It also belongs to the functionally defined scavenger receptor (SR) superfamily, whose members share the common ability to bind and internalize modified forms of Low Density Lipoproteins (LDL) (2-4). LOX-1 is the first member of the class E scavenger receptor subfamily (SR-E). It binds and supports the internalization of multiple structurally unrelated macromolecules including oxidized LDL, advanced glycation end products (AGE), activated platelets, bacteria, apoptotic or aged cells, and heat shock proteins (5-7). LOX-1 has also been implicated as an intestinal receptor involved in the transcytosis of pancreatic bile salt-dependent lipase (8). The mouse LOX-1 gene encodes a 363 amino acid (aa) residue protein with a short N-terminal intracellular domain, a transmembrane domain, triple repeats of an extracellular stalk/neck region followed by a C-type lectin-like domain (CTLD) (11). The CTLD, which is required for ligand recognition, contains the six conserved cysteine residues present in all C-type lectins, but lacks the Ca2+-binding residues found in classical C-type lectins. LOX-1 can be detected on activated endothelial cells, vascular smooth muscle cells, macrophages, intestinal cells and dendritic cells (6-8). The expression of LOX-1 is induced by pro‑inflammatory or pro‑atherogenic stimuli, as well as by oxidized LDL itself and hemodynamic or oxidative stress. LOX-1 exists on the cell surface as covalent homodimers, which can further associate into non-covalent-linked oligomers (9). Cell surface LOX-1 can also be cleaved by yet unidentified proteases to release the soluble LOX-1 extracellular domain (6). Binding and endocytosis of oxidized LDL by LOX-1 induces oxidative stress, activates NF kappa B, and upregulates the expression of monocyte chemoattractant protein-1 and matrix metalloproteases (5-9). LOX-1-dependent oxidized LDL uptake also induces apoptosis by inducing the expression of the pro‑apoptotic Bax and downregulation of the anti-apoptotic Bcl-2 (10). Oxidized LDL plays a key role in the pathogenesis of atherosclerosis and endothelial dysfunction. Blockade of LOX-1 functions may turn out to be a suitable target for the therapeutic intervention of atherosclerosis.

References
  1. Sawamura, T. et al. (1997) Nature 386:73.
  2. Daugherty, A. (2000) Curr. Opin. Cardiovasc. Pulm. Ren. Invest. Drugs. 2:223.
  3. Platt, N. and S. Gordon (2001) J. Clin. Invest. 108:649.
  4. Platt, N. and S. Gordon (1998) Chem. Biol. 5:R193.
  5. Jono, T. et al. (2002) FEBS Lett. 511:170.
  6. Kume, N. et al. (2001) Curr. Opin. Lipidol. 12:419.
  7. Delneste, Y. et al. (2002) Immunity 17:353.
  8. Bruneau, N. et al. (2003) Mol. Biol. Cell 14:2861.
  9. Xie, Q. et al. (2004) DNA and Cell Biol. 23:111.
  10. Chen, J. et al. (2003) Circ. Res. 94:370.
  11. Hoshikawa, H. et al. (1998) Biochem. Biophys. Res. Comm. 245:841.
Long Name
Lectin-like Oxidized Low-density Lipoprotein Receptor 1
Entrez Gene IDs
4973 (Human); 108078 (Mouse)
Alternate Names
CLEC8A; CLEC8ASLOX1; C-type lectin domain family 8 member A; hLOX-1; Lectin-like oxidized LDL receptor 1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1; LOX1; LOX-1; LOX1ox LDL receptor 1; LOXIN; OLR1; oxidised low density lipoprotein (lectin-like) receptor 1; oxidized low density lipoprotein (lectin-like) receptor 1; oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1, soluble form; Ox-LDL receptor 1; SCARE1; scavenger receptor class E, member 1; SR-E1

Product Datasheets

You must select a language.

x

Citations for Mouse LOX-1/OLR1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
Filter your results:

Filter by:

  1. Advanced glycation end-products reduce lipopolysaccharide uptake by macrophages
    Authors: A Kitaura, T Nishinaka, S Hamasaki, OF Hatipoglu, H Wake, M Nishibori, S Mori, S Nakao, H Takahashi
    PLoS ONE, 2021-01-25;16(1):e0245957.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Effect of Orally Administered Atractylodes macrocephala Koidz Water Extract on Macrophage and T Cell Inflammatory Response in Mice
    Authors: TK Kwak, HS Jang, MG Lee, YS Jung, DO Kim, YB Kim, JI Kim, H Kang
    Evid Based Complement Alternat Med, 2018-08-07;2018(0):4041873.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Effects of scavenger receptors-1 class A stimulation on macrophage morphology and highly modified advanced glycation end product-protein phagocytosis
    Authors: S Hamasaki, T Kobori, Y Yamazaki, A Kitaura, A Niwa, T Nishinaka, M Nishibori, S Mori, S Nakao, H Takahashi
    Sci Rep, 2018-04-12;8(1):5901.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Deletion of LOX-1 Protects against Heart Failure Induced by Doxorubicin
    PLoS ONE, 2016-05-19;11(5):e0154994.
    Species: Mouse
    Sample Types: Plasma
    Applications: Flow Cytometry
  5. Oxidation by neutrophils-derived HOCl increases immunogenicity of proteins by converting them into ligands of several endocytic receptors involved in antigen uptake by dendritic cells and macrophages.
    Authors: Biedron R, Konopinski M, Marcinkiewicz J, Jozefowski S
    PLoS ONE, 2015-04-07;10(4):e0123293.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse LOX-1/OLR1 PE-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse LOX-1/OLR1 PE-conjugated Antibody and earn rewards!

Have you used Mouse LOX-1/OLR1 PE-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review