Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 157108
View all Lymphotoxin beta R/TNFRSF3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Lymphotoxin beta R/TNFRSF3
Ser28-Pro218
Accession # P50284

Specificity

Detects mouse Lymphotoxin beta R in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse (rm) 4‑1BB, rmBAFF R, rmCD27, rmCD30, rmCD40, recombinant human (rh) DR3, rhDR6, rmEDAR, rmFas, rmGITR, rmHVEM, rhLymphotoxin  beta R, rmNGF R, rmOPG, rmOX40, rmRANK, rhRELT, rmTAJ, rmTNF RI, rmTNF RII, rhTRADD, rmTRAIL R2, rhTRAIL R3, rhTRAIL R4, or rmTWEAK R.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

Detection of Lymphotoxin beta R/TNFRSF3 antibody in NIH-3T3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Lymphotoxin beta R/TNFRSF3 in NIH‑3T3 Mouse Cell Line by Flow Cytometry.

NIH-3T3 mouse embryonic fibroblast cell line was stained with Rat Anti-Mouse Lymphotoxin beta R/TNFRSF3 Monoclonal Antibody (Catalog # MAB10081, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: NIH‑3T3 mouse embryonic fibroblast cell line

Western Blot

1 µg/mL
Sample: Recombinant Mouse Lymphotoxin  beta R/TNFRSF3
under non-reducing conditions only

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin beta R, now designated TNFRSF3, is a cell surface protein expressed in a variety of tissues. Lymphotoxin beta R transduces signals following binding of LIGHT or the heterotrimeric lymphotoxin LT alpha 1/ beta 2 or LT alpha 2/ beta 1. It plays a critical role in controlling cellular immune functions and lymphoid organogenesis.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

P50284

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

For research use only

Citations for Mouse Lymphotoxin  beta R/TNFRSF3 Antibody

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