Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 286820
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑6/Mcpt6
Ile32-Ser276
Accession # P21845

Specificity

Detects mouse Mast Cell Protease‑6/Mcpt6 in direct ELISAs and Western blots. In Western blots, 10% cross-reactivity with recombinant mouse (rm) MCP‑7 is observed and no cross-reactivity with rmMCP‑1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse Mast Cell Protease‑6/Mcpt6 Alexa Fluor® 532‑conjugated Antibody

Application
Recommended Usage

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Mast Cell Protease-6/Mcpt6

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast cell protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, Mcpt6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, Mcpt6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). Mcpt6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.

Alternate Names

Mast Cell Protease6, Mcpt6

Entrez Gene IDs

7177 (Human); 17229 (Mouse); 29268 (Rat)

Gene Symbol

TPSAB1

UniProt

Additional Mast Cell Protease-6/Mcpt6 Products

Product Documents for Mouse Mast Cell Protease‑6/Mcpt6 Alexa Fluor® 532‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Mast Cell Protease‑6/Mcpt6 Alexa Fluor® 532‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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