Mouse Mast Cell Protease-6/Mcpt6 Antibody

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Product Details
Citations (4)
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Mouse Mast Cell Protease-6/Mcpt6 Antibody Summary

Species Reactivity
Detects mouse Mast Cell Protease‑6/Mcpt6 in direct ELISAs and Western blots. In Western blots, 10% cross-reactivity with recombinant mouse (rm) MCP‑7 is observed and no cross-reactivity with rmMCP‑1 is observed.
Monoclonal Rat IgG1 Clone # 286820
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant mouse Mast Cell Protease‑6/Mcpt6
Accession # P21845
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (Catalog # 3736-SE)
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Mast Cell Protease‑6/Mcpt6 (Catalog # 3736-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Mast Cell Protease-6/Mcpt6

Tryptases are trypsin-like serine proteases, and beta tryptases appear to be the main isoenzymes expressed in mast cells (1). Mast cell protease-6 (MCP-6, Mcpt6, or Tpsb2) is one of beta tryptases produced from mouse mast cells. It is stored in secretory granules of mast cells, where it forms active tetramers with heparin proteoglycan. Because of the unique arrangement of the active sites in the tetramer, which are facing a narrow central pore, Mcpt6 is resistant to macromolecular protease inhibitors (2). When mast cells are activated, Mcpt6 is released along with other proteins in secretory granules, participating in provoking inflammatory conditions (3). Mcpt6 has been implicated as a mediator in the pathogenesis of asthma and other allergic disorders in mouse models.

  1. Caughey, G. H. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1535.
  2. Sommerhoff, C.P. et al. (1999) Proc. Natl. Acad. Sci. USA. 96:10984.
  3. Hallgren, J. and G. Pejler, (2006) FEBS J. 273:1871.
Entrez Gene IDs
7177 (Human); 17229 (Mouse); 29268 (Rat)
Alternate Names
Mast Cell Protease6; Mast Cell Protease-6; Mcpt6

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Citations for Mouse Mast Cell Protease-6/Mcpt6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. SLC10A4 regulates IgE-mediated mast cell degranulation in vitro and mast cell-mediated reactions in vivo
    Authors: H Pettersson, B Zarnegar, A Westin, V Persson, C Peuckert, J Jonsson, J Hallgren, K Kullander
    Sci Rep, 2017-04-24;7(1):1085.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. Tryptase - PAR2 axis in Experimental Autoimmune Prostatitis, a model for Chronic Pelvic Pain Syndrome
    Authors: Kenny Roman, Joseph D. Done, Anthony J. Schaeffer, Stephen F. Murphy, Praveen Thumbikat
  3. Basophils preferentially express mouse Mast Cell Protease 11 among the mast cell tryptase family in contrast to mast cells.
    Authors: Ugajin T, Kojima T, Mukai K, Obata K, Kawano Y, Minegishi Y, Eishi Y, Yokozeki H, Karasuyama H
    J. Leukoc. Biol., 2009-12-01;86(6):1417-25.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. VAMP-8 segregates mast cell-preformed mediator exocytosis from cytokine trafficking pathways.
    Authors: Tiwari N, Wang CC, Brochetta C, Ke G, Vita F, Qi Z, Rivera J, Soranzo MR, Zabucchi G, Hong W, Blank U
    Blood, 2008-01-18;111(7):3665-74.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC


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