Mouse Netrin-G2a Alexa Fluor® 405-conjugated Antibody

Catalog #: AF2744V Datasheet / COA / SDS

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AF2744V-100UG

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All Netrin-G2a Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Netrin-G2a Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Netrin-G2a in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Netrin-1, rmNetrin-G1a, and rmNetrin-4 is observed and less than 1%&nb

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Netrin-G2a
Asp18-Arg504
Accession # NP_598007

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Netrin-G2a

Mouse Netrin-G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin-G2a is approximately 485 amino acids (aa) in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF‑like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59 aa and 34 aa between EGF‑like motifs 1 and 2 generate Netrin-G2b and Netrin-G2c,respectively. Mature mouse Netrin-G2a shares 96% and 99% aa identity with human Netrin-G2 and the equivalent region in rat Netrin-G2b, respectively.

Entrez Gene IDs

84628 (Human); 171171 (Mouse)

Alternate Names

bA479K20.1;LHLL9381;Lmnt2;NEDBASH;NTNG1;NTNG2; Lmnt2a; NetrinG2a; Netrin-G2a; NTG2a