Mouse Netrin-G2a Antibody

Catalog # Availability Size / Price Qty
AF2744
AF2744-SP
Product Details
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Mouse Netrin-G2a Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Netrin-G2a in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Netrin-1, rmNetrin-G1a, and rmNetrin-4 is observed and less than 1% cross-reactivity with recombinant chicken Netrin-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Netrin-G2a
Asp18-Arg504
Accession # NP_598007
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Netrin-G2a

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Netrin-G2a

Mouse Netrin-G2a is a member of a GPI-linked Netrin subfamily that is distantly related to classical Netrins. Netrin-G2a is approximately 485 amino acids (aa) in length. It contains an N-terminal laminin-related region plus a heparin-binding C-terminus that ends in a GPI-linkage. The laminin-related region shows a globular type VI domain followed by three variable-length EGF‑like domains. Alternate splicing generates two larger isoforms. Amino acid insertions of 59 aa and 34 aa between EGF‑like motifs 1 and 2 generate Netrin-G2b and Netrin-G2c,respectively. Mature mouse Netrin-G2a shares 96% and 99% aa identity with human Netrin-G2 and the equivalent region in rat Netrin-G2b, respectively.

Entrez Gene IDs
84628 (Human); 171171 (Mouse)
Alternate Names
bA479K20.1;LHLL9381;Lmnt2;NEDBASH;NTNG1;NTNG2; Lmnt2a; NetrinG2a; Netrin-G2a; NTG2a

Product Datasheets

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Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 5,565,331, 6,096,866, 6,017,714, 6,309,638, 6,670,451, and other U.S. and international patents pending.

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody

WB,ELISA

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