Mouse P-Cadherin Alexa Fluor® 750-conjugated Antibody

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FAB761S-100UG
R&D Systems Antibodies
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Mouse P-Cadherin Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse P-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) N-Cadherin, recombinant mouse (rm) VE-Cadherin, rhCadherin-8, or rhCadherin-17 is observed.
Source
Monoclonal Rat IgG2a Clone # 106020
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse P-Cadherin
Glu100-Gly647
Accession # Q8BSL6
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
A431 human epithelial carcinoma cell line stained in buffer containing Ca2+ and Mg2+

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: P-Cadherin

Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules, designated CDH3. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region.

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.
  4. Nose, A. et al. (1987) EMBO J. 6:3655.
Long Name
Placental Cadherin
Entrez Gene IDs
1001 (Human); 12560 (Mouse); 116777 (Rat)
Alternate Names
CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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