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Mouse P-Cadherin Alexa Fluor® 594-conjugated Antibody

    
Datasheet / CoA / SDS
Detection of P‑Cadherin in A431 Human Cell Line by Flow Cytometry.

Flow Cytometry

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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse P-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) N-Cadherin, recombinant mouse (rm) VE-Cadherin, rhCadherin-8, or rhCadherin-17 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 106020
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse P-Cadherin Glu100-Gly647 Accession # Q8BSL6
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 594
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry P-Cadherin Antibody (106020) [Alexa Fluor® 594]
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Detection of P‑Cadherin in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Rat Anti-Human/Mouse P‑Cadherin Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB761T, filled histogram) or isotype control antibody (Catalog # IC006T, open histogram). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
      12 months from date of receipt, 2 to 8 degreesC as supplied.
Background: P-Cadherin
Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules, designated CDH3. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region.
  • References:
    1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
    2. Overduin, M. et al. (1995) Science 267:386.
    3. Takeichi, M. (1991) Science 251:1451.
    4. Nose, A. et al. (1987) EMBO J. 6:3655.
  • Long Name:
    Placental Cadherin
  • Entrez Gene IDs:
    1001 (Human); 12560 (Mouse); 116777 (Rat)
  • Alternate Names:
    CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 594-conjugated Antibody

 Ctrl IC006T
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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