Mouse P-Cadherin Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB761T-100UG
Detection  of P‑Cadherin in A431 Human Cell Line by Flow Cytometry.
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Mouse P-Cadherin Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse P-Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) N-Cadherin, recombinant mouse (rm) VE-Cadherin, rhCadherin-8, or rhCadherin-17 is observed.
Source
Monoclonal Rat IgG2A Clone # 106020
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse P-Cadherin
Glu100-Gly647
Accession # Q8BSL6
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of P‑Cadherin in A431 Human Cell Line by Flow Cytometry. View Larger

Detection of P‑Cadherin in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Rat Anti-Human/Mouse P‑Cadherin Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB761T, filled histogram) or isotype control antibody (Catalog # IC006T, open histogram). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: P-Cadherin

Placental Cadherin (P-Cadherin or PCAD) is a member of the cadherin family of cell adhesion molecules, designated CDH3. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region.

References
  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.
  4. Nose, A. et al. (1987) EMBO J. 6:3655.
Long Name
Placental Cadherin
Entrez Gene IDs
1001 (Human); 12560 (Mouse); 116777 (Rat)
Alternate Names
CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium-dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin

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