Human/Mouse P-Cadherin PE-conjugated Antibody Summary
Accession # P10287
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of P‑Cadherin in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Rat Anti-Human/Mouse P-Cadherin PE-conjugated Monoclonal Antibody (Catalog # FAB761P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Placental Cadherin (P-Cadherin), also known as PCAD and Cadherin-3, is a 120 kDa member of the cadherin family of cell adhesion molecules, designated CDH3. Cadherins are calcium-dependent transmembrane proteins, which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. P-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Constitutive P-Cadherin expression is found in the epidermis, mesothelium, corneal epithelium, and uterine decidua. Mouse P-Cadherin is an 822 amino acid (aa) protein with a 27 aa signal sequence and a 795 aa propeptide. The mature protein begins at aa 100 and has a 542 aa extracellular region, a 27 aa transmembrane region, and a 153 aa cytoplasmic region. Over aa 100-647, mouse P-Cadherin shares 94% and 85% aa sequence identity with rat and human P-Cadherin, respectively.
Citations for Human/Mouse P-Cadherin PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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The microRNA-200 family coordinately regulates cell adhesion and proliferation in hair morphogenesis
Authors: JE Hoefert, GA Bjerke, D Wang, R Yi
J. Cell Biol., 2018;0(0):.
Sample Types: Whole Cells
Applications: Flow Cytometry
Mobilizing transit-amplifying cell-derived ectopic progenitors prevents hair loss from chemotherapy or radiation therapy
Authors: WY Huang, SF Lai, HY Chiu, M Chang, M Plikus, CC Chan, YT Chen, PN Tsao, TL Yang, HS Lee, P Chi, SJ Lin
Cancer Res., 2017;0(0):.
Sample Types: Tissue Homogenates
Applications: Flow Cytometry
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