Mouse PDGF R alpha Alexa Fluor® 488-conjugated Antibody Summary
Leu25-Glu524 (Asp65Glu, Gly439Ala, Thr440Ala), predicted
Accession # P26618
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of PDGF R alpha in Mouse Bone Marrow by Flow Cytometry. Mouse bone marrow was stained with Rat Anti-Mouse CD45 APC-conjugated Monoclonal Antibody (Catalog # FAB114A) and either (A) Rat Anti-Mouse PDGF Ra Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB9215G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
Detection of PDGF R alpha in Mouse Compact Bone Tissue by Flow Cytometry. Mouse compact bone tissue was stained with Rat Anti-Mouse CD45 APC-conjugated Monoclonal Antibody (Catalog # FAB114A) and either (A) Rat Anti-Mouse PDGF Ra Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB9215G) or (B) Rat IgG2AAlexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PDGF R alpha
PDGF R alpha (Platelet-Derived Growth Factor Receptor alpha), also known as CD140a, is a 165-170 kDa member of the PDGFR (or class III) subfamily, Tyrosine Kinase family, Protein Kinase superfamily of molecules. Mature mouse PDGF R alpha is a 1065 amino acid (aa) type I transmembrane glycoprotein that contains five Ig-like domains within a 504 aa extracellular domain. It is expressed on a wide variety of cell types, both fetal and adult. In the fetus, PDGF R alpha is found on NG2+ oligodendroglial precursors, mesenchyme of both the sclerotome and dermatome (but not myotome), mesenchyme of the lung, hepatoblasts, Leydig cells, and neural crest cells that target the branchial arches. In the adult, PDGF R alpha has been found constitutively or inducibly on hepatocytes, vascular and visceral smooth muscle, Leydig cells, retinal astrocytes, fibroblasts, monocytes, hepatic stellate cells, CD44+ adipocyte precursors, endothelial cells, osteoblasts, and Ptch+ Gli+ pericryptal colonic subepithelial fibroblasts. On the cell surface, PDGF R alpha is a monomer. Upon binding to a dimeric ligand, PDGF R alpha either homodimerizes, or heterodimerizes with PDGF R beta. The PDGF R alpha homodimer binds PDGF-AA, -AB, -BB, and -CC, while the alpha beta -heterodimer binds PDGF-AB, -BB, and -CC. Over aa 25-524, mouse PDGF R alpha shares 93% and 85% aa sequence identity with rat and human PDGF R alpha, respectively. Over the same aa sequence, mouse PDGF R alpha shares only 33% aa sequence identity with mouse PDGF R beta.
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
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