Mouse Peptidase Inhibitor 16/PI16 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF4929G Datasheet / COA / SDS

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AF4929G-100UG

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All Peptidase Inhibitor 16/PI16 Antibodies

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Mouse Peptidase Inhibitor 16/PI16 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Peptidase Inhibitor 16/PI16 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Peptidase Inhibitor 16/PI16
Leu21-Glu464
Accession # Q9ET66

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Peptidase Inhibitor 16/PI16

PI16 (Peptidase Inhibitor 16) is a variable molecular weight (MW) member of the CRISP family of proteins. It is expressed by cardiomyocytes and serves as an autocrine negative growth regulator. Mature mouse PI16 is a type I transmembrane protein 469 amino acids (aa) in length. The extracellular region includes all but the C‑terminal 21 amino acids. A soluble form of PI16 is known. Although its predicted MW is 50 kDa, it runs anomalously at 70 kDa (unmodified) in SDS-PAGE. With glycosylation, it appears to be 100‑110 kDa in size. Three potential variants exist. One shows a deletion of aa 51‑124, a second shows a 9 aa extension at the N‑terminus, and a third shows a deletion of aa 191‑452. Over aa 1‑464, mouse PI16 shares 86% and 61% aa identity with rat and human PI16, respectively.

Entrez Gene IDs

221476 (Human); 74116 (Mouse); 294312 (Rat)

Alternate Names

CRISP9; CRISP-9; Cysteine-rich secretory protein 9; dJ90K10.5; DKFZp586B1817; MGC45378; microseminoprotein, beta-binding protein; MSMBBP; Peptidase Inhibitor 16; PI16; PI-16; protease inhibitor 16; PSP94-binding protein; PSPBP