Mouse PLZF Alexa Fluor® 647-conjugated Antibody IC83951R-100UG: R&D Systems

Mouse PLZF Alexa Fluor® 647-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PLZF in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 816421
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse PLZF
    Met1-Gln254
    Accession # NP_001028496
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    Please refer to the PDF datasheet for specifications.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of PLZF in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes stimulated with Recombinant Mouse IL‑12 (Catalog # 419-ML)and Recombinant Mouse IL‑18 (Catalog #
9139-IL) for 3 days were stained with Rat Anti-Mouse NKp46/NCR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB22252P) and either (A) Rat Anti-Mouse PLZF Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC83951R) or (B) Rat IgG2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PLZF
Mouse PLZF, also known as Zinc finger and BTB domain containing protein 16 (ZBTB-16) PLZF and ZNF145, is a 74 kDa nuclear protein that belongs to the POK family of transcriptional repressors. It is a 673 amino acid (aa) protein that contains an N-terminal BTB domain, followed by an acidic domain, a proline-rich region and a C-terminal zinc finger domain. PLZF forms dimers with RAR alpha and LAZ3 within its zinc finger region. Alternate splice forms exist that are tissue-specific and show a deletion of either the BTB domain, the acidic region, or the proline-rich region. PLZF is highly expressed in undifferentiated, multi-potential hematopoietic progenitor cells, with levels declining as cells commit to various lineages. Mouse PLZF shares 98% and 96% aa identity with rat and human PLZF, respectively.
  • Long Name:
    Promyelocytic Leukemia Zinc Finger
  • Entrez Gene IDs:
    7704 (Human); 235320 (Mouse)
  • Alternate Names:
    PLZF; PLZFzinc finger and BTB domain-containing protein 16; ZBTB16; zinc finger and BTB domain containing 16; zinc finger protein 145 (Kruppel-like, expressed in promyelocytic leukemia); Zinc finger protein 145; Zinc finger protein PLZF; ZNF145; ZNF145Promyelocytic leukemia zinc finger protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 647-conjugated Antibody

Ctrl IC006R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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