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Recombinant Mouse IL-12 Protein

R&D Systems | Catalog # 419-ML

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Key Product Details

  • R&D Systems Sf 21 (baculovirus)-derived Recombinant Mouse IL-12 Protein (419-ML)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

Sf 21 (baculovirus)

Accession Number

P43432 (p40) & NP_032377 (p35)

Structure / Form

Disulfide-linked heterodimer

Applications

Bioactivity
View all IL-12 Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse IL-12 protein
Mouse IL-12 p40
(Met23-Ser335)
Accession # P43432
Mouse IL-12 p35
(Arg23-Ala215)
Accession # NP_032377
N-terminus C-terminus

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met23 (p40) & Arg23 (p35)

SDS-PAGE

70 kDa, non-reducing conditions

Activity

Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202.
The ED50 for this effect is 0.01-0.1 ng/mL.

Reviewed Applications

Read 9 reviews rated 4.8 using 419-ML in the following applications:

Scientific Data Images for Recombinant Mouse IL-12 Protein

Recombinant Mouse IL-12 Protein Bioactivity

Recombinant Mouse IL-12 Protein Bioactivity

Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01‑0.1 ng/mL.
Recombinant Mouse IL-12 Protein SDS-PAGE

Recombinant Mouse IL-12 Protein SDS-PAGE

1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 25 kDa and 43 kDa (R) and 68 kDa (NR).

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 419-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 419-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-12

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

Long Name

Interleukin 12

Alternate Names

CLMF1, IL12, IL12A, NKSF1

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat); 397053 (Porcine); 403977 (Canine); 102115316 (Cynomolgus Monkey); 493741 (Feline); 703205 (Rhesus Macaque)

Gene Symbol

IL12A

UniProt

P43432 (p40) & NP_032377 (p35)

Additional IL-12 Products

Product Documents for Recombinant Mouse IL-12 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 419-ML (with carrier)
Download SDS 419-ML/CF (carrier free)

Product Specific Notices for Recombinant Mouse IL-12 Protein

For research use only

Citations for Recombinant Mouse IL-12 Protein

Customer Reviews for Recombinant Mouse IL-12 Protein (9)

4.8 out of 5
9 Customer Ratings
5 Stars
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4 Stars
22%
3 Stars
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1 Stars
0%

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  • Recombinant Mouse IL-12 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 09/08/2024
    Splenocytes from naive Balb/c mice were cultured with mDF6006 or rmIL-12 for 60 h. IFN gamma in supernatant was analyzed.
    Recombinant Mouse IL-12 Protein 419-ML
  • Recombinant Mouse IL-12 Protein
    Name: Khuynh Nguyen
    Application: In vivo study
    Verified Customer | Posted 10/06/2023
    Recombinant Mouse IL-12 Protein 419-ML
  • Recombinant Mouse IL-12 Protein
    Name: Gagandeep Kaur
    Application: Cell Proliferation
    Verified Customer | Posted 11/09/2020
  • Recombinant Mouse IL-12 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/23/2020
    Recombinant Mouse IL-12 Protein 419-ML
  • Recombinant Mouse IL-12 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 12/02/2019
    Recombinant Mouse IL-12 Protein 419-ML
  • Recombinant Mouse IL-12 Protein
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 01/22/2018
  • Recombinant Mouse IL-12 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/27/2017
    This protein was used as the calibrator in an ELISA using MAB419 and BAF419 as the matched pair antibodies.
    Recombinant Mouse IL-12 Protein 419-ML
  • Recombinant Mouse IL-12 Protein
    Name: Anonymous
    Application: CD4+ T cell polarization toward Th1
    Verified Customer | Posted 08/24/2017
    Recombinant Mouse IL-12 Protein 419-ML
  • Recombinant Mouse IL-12 Protein
    Name: SOjan Abraham
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/21/2016

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FAQs for Recombinant Mouse IL-12 Protein

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Is there a mass to unit conversion factor for Recombinant Mouse IL-12 Protein (Catalog # 419-ML & 419-ML/CF)?

    A: We have not developed a specific mass to unit conversion factor for Recombinant Mouse IL-12 Protein (Catalog # 419-ML & 419-ML/CF).  Our best recommendation is to perform a dose-response study for your assay to determine the optimal concentration for your experiment.  We recommend checking the Citations tab on the product-specific page to review publications citing use of the product.

  • Q: Should I use Recombinant Mouse IL-12 Protein formulated with carrier BSA (Catalog # 419-ML) or the Recombinant Mouse IL-12 Protein, CF (Catalog # 419-ML/CF) that is carrier-free (no BSA) for my in vivo experiments?

    A: For in vivo aplications, we would recommend using the carrier-free Recombinant Mouse IL-12 Protein (Catalog # 419-ML/CF). The injection of a carrier protein such as BSA can cause side effects and sometimes death to the experimental animal. For more information regarding use of the protein for in vivo applications, we would recommend consulting the Citations tab on the product-specific page.

  • Q: What is the extra band observed when running Recombinant Mouse IL-12 Protein (Catalog # 419-ML) in Western blot?

    A: Recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE.

  • Q: Is there a mass to unit conversion factor for Recombinant Mouse IL-12 Protein (Catalog # 419-ML & 419-ML/CF)?

    A: We have not developed a specific mass to unit conversion factor for Recombinant Mouse IL-12 Protein (Catalog # 419-ML & 419-ML/CF).  Our best recommendation is to perform a dose-response study for your assay to determine the optimal concentration for your experiment.  We recommend checking the Citations tab on the product-specific page to review publications citing use of the product.

  • Q: Should I use Recombinant Mouse IL-12 Protein formulated with carrier BSA (Catalog # 419-ML) or the Recombinant Mouse IL-12 Protein, CF (Catalog # 419-ML/CF) that is carrier-free (no BSA) for my in vivo experiments?

    A: For in vivo aplications, we would recommend using the carrier-free Recombinant Mouse IL-12 Protein (Catalog # 419-ML/CF). The injection of a carrier protein such as BSA can cause side effects and sometimes death to the experimental animal. For more information regarding use of the protein for in vivo applications, we would recommend consulting the Citations tab on the product-specific page.

  • Q: What is the extra band observed when running Recombinant Mouse IL-12 Protein (Catalog # 419-ML) in Western blot?

    A: Recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE.

  • Q: Is there a mass to unit conversion factor for Recombinant Mouse IL-12 Protein (Catalog # 419-ML & 419-ML/CF)?

    A: We have not developed a specific mass to unit conversion factor for Recombinant Mouse IL-12 Protein (Catalog # 419-ML & 419-ML/CF).  Our best recommendation is to perform a dose-response study for your assay to determine the optimal concentration for your experiment.  We recommend checking the Citations tab on the product-specific page to review publications citing use of the product.

  • Q: Should I use Recombinant Mouse IL-12 Protein formulated with carrier BSA (Catalog # 419-ML) or the Recombinant Mouse IL-12 Protein, CF (Catalog # 419-ML/CF) that is carrier-free (no BSA) for my in vivo experiments?

    A: For in vivo aplications, we would recommend using the carrier-free Recombinant Mouse IL-12 Protein (Catalog # 419-ML/CF). The injection of a carrier protein such as BSA can cause side effects and sometimes death to the experimental animal. For more information regarding use of the protein for in vivo applications, we would recommend consulting the Citations tab on the product-specific page.

  • Q: What is the extra band observed when running Recombinant Mouse IL-12 Protein (Catalog # 419-ML) in Western blot?

    A: Recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE.

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