Recombinant Mouse IL-12 Protein

(156 citations)
(3 Reviews)
  
  • Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using PHA-activated mouse splenocytes. Mattner, F. et al. (1993) Eur. J. Immunol. 23:2202. The ED50 for this effect is 0.01-0.1 ng/mL.
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived
    Mouse IL-12 p40
    (Met23-Ser335)
    Accession # P43432
    Mouse IL-12 p35
    (Arg23-Ala215)
    Accession # NP_032377
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis

    Met23 (p40) & Arg23 (p35)

  • Structure / Form
    Disulfide-linked heterodimer
  • SDS-PAGE
    70 kDa, non-reducing conditions
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
419-ML
 
419-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse IL-12 (Catalog # 419‑ML) stimulates cell proliferation of PHA-activated mouse splenocytes. The ED50 for this effect is 0.01‑0.1 ng/mL.
1 μg/lane of Recombinant Mouse IL-12 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 25 kDa and 43 kDa (R) and 68 kDa (NR).
Background: IL-12

Interleukin 12, also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL‑12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL‑12 activities include the induction of IFN‑ gamma and TNF in resting and activated T and NK cells; the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen; and the enhancement of NK cell proliferation. Current evidence indicates that IL‑12 is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL‑12 may have therapeutic potential as a vaccine adjuvant that promotes cellular-immunity and as an anti-tumor and anti-viral agent.

Human and mouse IL‑12 share 70% and 60% amino acid sequence identity in their p40 and p35 subunits, respectively. While mouse IL‑12 is active on both human and mouse cells, human IL‑12 is not active on murine cells. R&D Systems' recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE. The biological activity of this cleaved mouse IL‑12 is comparable to that of the intact human IL‑12.

  • Long Name:
    Interleukin 12
  • Entrez Gene IDs:
    3592 (Human); 16159 (Mouse); 84405 (Rat); 403977 (Canine); 493741 (Feline)
  • Alternate Names:
    CLMF p35; CLMF1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; IL12; IL-12; IL-12, subunit p35; IL12A; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocytematuration factor 1, p35); interleukin-12 alpha chain; interleukin-12 subunit alpha; natural killer cell stimulatory factor 1, 35 kD subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; NKSF1; p35
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

156 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Lamtor1 Is Critically Required for CD4(+) T Cell Proliferation and Regulatory T Cell Suppressive Function
    Authors: T Hosokawa, T Kimura, S Nada, T Okuno, D Ito, S Kang, S Nojima, K Yamashita, T Nakatani, Y Hayama, Y Kato, Y Kinehara, M Nishide, N Mikami, S Koyama, H Takamatsu, D Okuzaki, N Ohkura, S Sakaguchi, M Okada, A Kumanogoh
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. Manipulating Glucose Metabolism during Different Stages of Viral Pathogenesis Can Have either Detrimental or Beneficial Effects
    Authors: SK Varanasi, D Donohoe, U Jaggi, BT Rouse
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. The transcriptional coactivator TAZ regulates reciprocal differentiation of TH17 cells and Treg cells
    Authors: J Geng, S Yu, H Zhao, X Sun, X Li, P Wang, X Xiong, L Hong, C Xie, J Gao, Y Shi, J Peng, RL Johnson, N Xiao, L Lu, J Han, D Zhou, L Chen
    Nat. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Dectin 1 activation on macrophages by galectin 9 promotes pancreatic carcinoma and peritumoral immune tolerance
    Authors: D Daley, VR Mani, N Mohan, N Akkad, A Ochi, DW Heindel, KB Lee, CP Zambirinis, GSB Pandian, S Savadkar, A Torres-Her, S Nayak, D Wang, M Hundeyin, B Diskin, B Aykut, G Werba, RM Barilla, R Rodriguez, S Chang, L Gardner, LK Mahal, B Ueberheide, G Miller
    Nat. Med., 2017;23(5):556-567.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Absence of Notch1 in murine myeloid cells attenuates the development of experimental autoimmune encephalomyelitis by affecting Th1 and Th17 priming
    Authors: M Fernández, EM Monsalve, S López-Lópe, A Ruiz-Garcí, S Mellado, E Caminos, JJ García-Ram, J Laborda, P Tranque, MJM Díaz-Guerr
    Eur. J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Dual role of ALCAM in neuroinflammation and blood-brain barrier homeostasis
    Authors: MA L‚cuyer, O Saint-Laur, L BourbonniŠ, S Larouche, C Larochelle, L Michel, M Charabati, M Abadier, S Zandee, N Haghayegh, E Gowing, C Pittet, R Lyck, B Engelhardt, A Prat
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E524-E533.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Gut memories do not fade: epigenetic regulation of lasting gut homing receptor expression in CD4(+) memory T cells
    Authors: BA Szilagyi, J Triebus, C Kressler, M de Almeida, S Tierling, P Durek, M Mardahl, A Szilagyi, S Floess, J Huehn, U Syrbe, J Walter, JK Polansky, A Hamann
    Mucosal Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Hypoxia Enhances Immunosuppression by Inhibiting CD4+ Effector T Cell Function and Promoting Treg Activity
    Authors: AM Westendorf, K Skibbe, A Adamczyk, J Buer, R Geffers, W Hansen, E Pastille, V Jendrossek
    Cell. Physiol. Biochem, 2017;41(4):1271-1284.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. BACH2 immunodeficiency illustrates an association between super-enhancers and haploinsufficiency
    Authors: B Afzali, J Grönholm, J Vandrovcov, C O'Brien, HW Sun, I Vanderleyd, FP Davis, A Khoder, Y Zhang, AN Hegazy, AV Villarino, IW Palmer, J Kaufman, NR Watts, M Kazemian, O Kamenyeva, J Keith, A Sayed, D Kasperavic, M Mueller, JD Hughes, IJ Fuss, MF Sadiyah, K Montgomery, J McElwee, NP Restifo, W Strober, MA Linterman, PT Wingfield, HH Uhlig, R Roychoudhu, TJ Aitman, P Kelleher, MJ Lenardo, JJ O'Shea, N Cooper, ADJ Laurence
    Nat. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. Armc5 deletion causes developmental defects and compromises T-cell immune responses
    Authors: Y Hu, L Lao, J Mao, W Jin, H Luo, T Charpentie, S Qi, J Peng, B Hu, MM Marcinkiew, A Lamarre, J Wu
    Nat Commun, 2017;8(0):13834.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. A Murine Intestinal Intraepithelial NKp46-Negative Innate Lymphoid Cell Population Characterized by Group 1 Properties
    Authors: A Van Acker, K Gronke, A Biswas, L Martens, Y Saeys, J Filtjens, S Taveirne, E Van Ammel, T Kerre, P Matthys, T Taghon, B Vandekerck, J Plum, IR Dunay, A Diefenbach, G Leclercq
    Cell Rep, 2017;19(7):1431-1443.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. The RNA-binding protein HuR contributes to neuroinflammation by promoting C-C chemokine receptor 6 (CCR6) expression on Th17 cells
    Authors: J Chen, JL Martindale, C Cramer, M Gorospe, U Atasoy, PD Drew, S Yu
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. Induction of Colonic Regulatory T Cells by Mesalamine by Activating the Aryl Hydrocarbon Receptor
    Authors: K Oh-Oka, Y Kojima, K Uchida, K Yoda, K Ishimaru, S Nakajima, J Hemmi, H Kano, Y Fujii-Kuri, R Katoh, H Ito, A Nakao
    Cell Mol Gastroenterol Hepatol, 2017;4(1):135-151.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. TLR7 Signaling Regulates Th17 Cells and Autoimmunity: Novel Potential for Autoimmune Therapy
    Authors: J Ye, Y Wang, X Liu, L Li, A Opejin, EC Hsueh, H Luo, T Wang, D Hawiger, G Peng
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. Egr2 and 3 Inhibit T-bet-Mediated IFN-? Production in T Cells
    Authors: R Singh, T Miao, ALJ Symonds, B Omodho, S Li, P Wang
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. A role for cathepsin Z in neuroinflammation provides mechanistic support for an epigenetic risk factor in multiple sclerosis
    Authors: ERO Allan, RI Campden, BW Ewanchuk, P Tailor, DR Balce, NT McKenna, CJ Greene, AL Warren, T Reinheckel, RM Yates
    J Neuroinflammation, 2017;14(1):103.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. RGS10 deficiency ameliorates the severity of disease in experimental autoimmune encephalomyelitis.
    Authors: Lee J, Kannarkat G, Chung J, Joon Lee H, Graham K, Tansey M
    J Neuroinflammation, 2016;13(1):24.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Mesothelin-specific cell-based vaccine generates antigen-specific immunity and potent antitumor effects by combining with IL-12 immunomodulator.
    Authors: Chang M, Chen Y, Chiang Y, Chen T, Tang Y, Chen C, Sun W, Cheng W
    Gene Ther, 2016;23(1):38-49.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  19. Inflammation enhances the vaccination potential of CD40-activated B cells in mice
    Eur. J. Immunol., 2016;0(0):2016.
    Species: Mouse
    Sample Type: In Vivo
  20. Th1 cells downregulate connexin 43 gap junctions in astrocytes via microglial activation
    Sci Rep, 2016;6(0):38387.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. Attenuation of PKR-like ER Kinase (PERK) Signaling Selectively Controls Endoplasmic Reticulum Stress-induced Inflammation Without Compromising Immunological Responses
    Authors: LN Guthrie, K Abiraman, ES Plyler, NT Sprenkle, SA Gibson, BC McFarland, R Rajbhandar, AL Rowse, EN Benveniste, GP Meares
    J. Biol. Chem., 2016;291(30):15830-40.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  22. T cells are necessary for ILC2 activation in house dust mite-induced allergic airway inflammation in mice
    Authors: BW Li, MJ de Bruijn, I Tindemans, M Lukkes, A KleinJan, HC Hoogsteden, RW Hendriks
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. Autoantibody-boosted T-cell reactivation in the target organ triggers manifestation of autoimmune CNS disease
    Proc. Natl. Acad. Sci. U.S.A., 2016;113(12):3323-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  24. Immune signatures of protective spleen memory CD8 T cells
    Sci Rep, 2016;6(0):37651.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  25. p(70S6K1) in the TORC1 pathway is essential for the differentiation of Th17 Cells, but not Th1, Th2, or Treg cells in mice.
    Authors: Sasaki C, Chen G, Munk R, Eitan E, Martindale J, Longo D, Ghosh P
    Eur J Immunol, 2016;46(1):212-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  26. Dimethyl fumarate treatment induces adaptive and innate immune modulation independent of Nrf2
    Authors: U Schulze-To, M Varrin-Doy, K Pekarek, CM Spencer, A Shetty, SA Sagan, BA Cree, RA Sobel, BT Wipke, L Steinman, RH Scannevin, SS Zamvil
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  27. Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells
    Nat Commun, 2016;7(0):11626.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. Conditioned Medium from the Stem Cells of Human Exfoliated Deciduous Teeth Ameliorates Experimental Autoimmune Encephalomyelitis
    Authors: C Shimojima, H Takeuchi, S Jin, B Parajuli, H Hattori, A Suzumura, H Hibi, M Ueda, A Yamamoto
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  29. Blockade of Extracellular ATP Effect by Oxidized ATP Effectively Mitigated Induced Mouse Experimental Autoimmune Uveitis (EAU)
    PLoS ONE, 2016;11(5):e0155953.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  30. Adaptive immunity against gut microbiota enhances apoE-mediated immune regulation and reduces atherosclerosis and western-diet-related inflammation
    Sci Rep, 2016;6(0):29353.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  31. Myelin-specific Th17 cells induce severe relapsing optic neuritis with irreversible loss of retinal ganglion cells in C57BL/6 mice
    Authors: CM Larabee, Y Hu, S Desai, C Georgescu, JD Wren, RC Axtell, SM Plafker
    Mol Vis, 2016;22(0):332-41.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  32. Interleukin-12 inhibits pathological neovascularization in mouse model of oxygen-induced retinopathy
    Authors: Yedi Zhou
    Sci Rep, 2016;6(0):28140.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  33. IL-18R?-deficient CD4(+) T cells induce intestinal inflammation in the CD45RB(hi) transfer model of colitis despite impaired innate responsiveness
    Authors: P Holmkvist, L Pool, K Hägerbrand, WW Agace, A Rivollier
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  34. An IL-27/NFIL3 signalling axis drives Tim-3 and IL-10 expression and T-cell dysfunction.
    Authors: Zhu C, Sakuishi K, Xiao S, Sun Z, Zaghouani S, Gu G, Wang C, Tan D, Wu C, Rangachari M, Pertel T, Jin H, Ahmed R, Anderson A, Kuchroo V
    Nat Commun, 2015;6(0):6072.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  35. Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells.
    Authors: Rutz S, Kayagaki N, Phung Q, Eidenschenk C, Noubade R, Wang X, Lesch J, Lu R, Newton K, Huang O, Cochran A, Vasser M, Fauber B, DeVoss J, Webster J, Diehl L, Modrusan Z, Kirkpatrick D, Lill J, Ouyang W, Dixit V
    Nature, 2015;518(7539):417-21.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  36. miR-148a is upregulated by Twist1 and T-bet and promotes Th1-cell survival by regulating the proapoptotic gene Bim.
    Authors: Haftmann C, Stittrich A, Zimmermann J, Fang Z, Hradilkova K, Bardua M, Westendorf K, Heinz G, Riedel R, Siede J, Lehmann K, Weinberger E, Zimmel D, Lauer U, Haupl T, Sieper J, Backhaus M, Neumann C, Hoffmann U, Porstner M, Chen W, Grun J, Baumgrass R, Matz M, Lohning M, Scheffold A, Wittmann J, Chang H, Rajewsky N, Jack H, Radbruch A, Mashreghi M
    Eur J Immunol, 2015;45(4):1192-205.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  37. DNA-dependent protein kinase inhibition blocks asthma in mice and modulates human endothelial and CD4(+) T-cell function without causing severe combined immunodeficiency.
    Authors: Ghonim M, Pyakurel K, Ju J, Rodriguez P, Lammi M, Davis C, Abughazleh M, Mansy M, Naura A, Boulares A
    J Allergy Clin Immunol, 2015;135(2):425-40.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  38. TGF-beta3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.
    Authors: Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K
    Nat Commun, 2015;6(0):6329.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  39. Dynamics of Circulating gammadelta T Cell Activity in an Immunocompetent Mouse Model of High-Grade Glioma.
    Authors: Beck B, Kim H, O'Brien R, Jadus M, Gillespie G, Cloud G, Hoa N, Langford C, Lopez R, Harkins L, Lamb L
    PLoS ONE, 2015;10(5):e0122387.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  40. NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.
    Authors: Luci C, Bekri S, Bihl F, Pini J, Bourdely P, Nouhen K, Malgogne A, Walzer T, Braud V, Anjuere F
    PLoS ONE, 2015;10(12):e0143224.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  41. ROR inverse agonist suppresses insulitis and prevents hyperglycemia in a mouse model of type 1 diabetes.
    Authors: Solt L, Banerjee S, Campbell S, Kamenecka T, Burris T
    Endocrinology, 2015;156(3):869-81.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  42. Identification and Analysis of Natural Killer Cells in Murine Nasal Passages.
    Authors: Okada K, Sato S, Sato A, Mandelboim O, Yamasoba T, Kiyono H
    PLoS ONE, 2015;10(11):e0142920.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  43. MHCII-independent CD4+ T cells protect injured CNS neurons via IL-4.
    Authors: Walsh J, Hendrix S, Boato F, Smirnov I, Zheng J, Lukens J, Gadani S, Hechler D, Golz G, Rosenberger K, Kammertons T, Vogt J, Vogelaar C, Siffrin V, Radjavi A, Fernandez-Castaneda A, Gaultier A, Gold R, Kanneganti T, Nitsch R, Zipp F, Kipnis J
    J Clin Invest, 2015;125(2):699-714.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  44. Interleukin-36gamma is expressed by neutrophils and can activate microglia, but has no role in experimental autoimmune encephalomyelitis.
    Authors: Bozoyan L, Dumas A, Patenaude A, Vallieres L
    J Neuroinflammation, 2015;12(0):173.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  45. Immune requirements for protective Th17 recall responses to Mycobacterium tuberculosis challenge.
    Authors: Monin L, Griffiths K, Slight S, Lin Y, Rangel-Moreno J, Khader S
    Mucosal Immunol, 2015;8(5):1099-109.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  46. IL-33 receptor ST2 amplifies the expansion of NK cells and enhances host defense during mouse cytomegalovirus infection.
    Authors: Nabekura T, Girard J, Lanier L
    J Immunol, 2015;194(12):5948-52.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  47. Effector T Cell Egress via Afferent Lymph Modulates Local Tissue Inflammation.
    Authors: Gomez D, Diehl M, Crosby E, Weinkopff T, Debes G
    J Immunol, 2015;195(8):3531-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  48. Adaptive Immune-like gamma/delta T Lymphocytes Share Many Common Features with Their alpha/beta T Cell Counterparts.
    Authors: Lombes A, Durand A, Charvet C, Riviere M, Bonilla N, Auffray C, Lucas B, Martin B
    J Immunol, 2015;195(4):1449-58.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  49. CXCL13 antibody for the treatment of autoimmune disorders.
    Authors: Klimatcheva E, Pandina T, Reilly C, Torno S, Bussler H, Scrivens M, Jonason A, Mallow C, Doherty M, Paris M, Smith E, Zauderer M
    BMC Immunol, 2015;16(0):6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  50. Mesenchymal stem/stromal cells protect against autoimmunity via CCL2-dependent recruitment of myeloid-derived suppressor cells.
    Authors: Lee H, Ko J, Jeong H, Ko A, Kim M, Wee W, Yoon S, Oh J
    J Immunol, 2015;194(8):3634-45.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  51. IkappaBNS regulates murine Th17 differentiation during gut inflammation and infection.
    Authors: Annemann M, Wang Z, Plaza-Sirvent C, Glauben R, Schuster M, Ewald Sander F, Mamareli P, Kuhl A, Siegmund B, Lochner M, Schmitz I
    J Immunol, 2015;194(6):2888-98.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  52. miR-20b suppresses Th17 differentiation and the pathogenesis of experimental autoimmune encephalomyelitis by targeting RORgammat and STAT3.
    Authors: Zhu E, Wang X, Zheng B, Wang Q, Hao J, Chen S, Zhao Q, Zhao L, Wu Z, Yin Z
    J Immunol, 2014;192(12):5599-609.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  53. Cytomegalovirus generates long-lived antigen-specific NK cells with diminished bystander activation to heterologous infection.
    Authors: Min-Oo G, Lanier L
    J Exp Med, 2014;211(13):2669-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  54. Therapeutic efficacy of suppressing the Jak/STAT pathway in multiple models of experimental autoimmune encephalomyelitis.
    Authors: Liu Y, Holdbrooks A, De Sarno P, Rowse A, Yanagisawa L, McFarland B, Harrington L, Raman C, Sabbaj S, Benveniste E, Qin H
    J Immunol, 2014;192(1):59-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  55. Dok1 and Dok2 proteins regulate natural killer cell development and function.
    Authors: Celis-Gutierrez J, Boyron M, Walzer T, Pandolfi P, Jonjic S, Olive D, Dalod M, Vivier E, Nunes J
    EMBO J, 2014;33(17):1928-40.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  56. Regulation of experimental autoimmune encephalomyelitis by TPL-2 kinase.
    Authors: Sriskantharajah S, Guckel E, Tsakiri N, Kierdorf K, Brender C, Ben-Addi A, Veldhoen M, Tsichlis P, Stockinger B, O'Garra A, Prinz M, Kollias G, Ley S
    J Immunol, 2014;192(8):3518-29.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  57. The transcription factor Zbtb32 controls the proliferative burst of virus-specific natural killer cells responding to infection.
    Authors: Beaulieu A, Zawislak C, Nakayama T, Sun J
    Nat Immunol, 2014;15(6):546-53.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  58. Transcription factor Runx3 regulates interleukin-15-dependent natural killer cell activation.
    Authors: Levanon, Ditsa, Negreanu, Varda, Lotem, Joseph, Bone, Karen Ra, Brenner, Ori, Leshkowitz, Dena, Groner, Yoram
    Mol Cell Biol, 2014;34(6):1158-69.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  59. A novel CMKLR1 small molecule antagonist suppresses CNS autoimmune inflammatory disease.
    Authors: Graham K, Zhang J, Lewen S, Burke T, Dang T, Zoudilova M, Sobel R, Butcher E, Zabel B
    PLoS ONE, 2014;9(12):e112925.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  60. Islet antigen-specific Th17 cells can induce TNF-alpha-dependent autoimmune diabetes.
    Authors: Li C, Mueller E, Bradley L
    J Immunol, 2014;192(4):1425-32.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  61. Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
    Authors: Reynolds C, Chong D, Raynsford E, Quigley K, Kelly D, Llewellyn-Hughes J, Altmann D, Boyton R
    BMC Biol, 2014;12(0):32.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  62. Site-specific chemokine expression regulates central nervous system inflammation and determines clinical phenotype in autoimmune encephalomyelitis.
    Authors: Stoolman J, Duncker P, Huber A, Segal B
    J Immunol, 2014;193(2):564-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  63. IL-18 expression results in a recombinant vaccinia virus that is highly attenuated and immunogenic.
    Authors: Verardi P, Legrand F, Chan K, Peng Y, Jones L, Yilma T
    J Interferon Cytokine Res, 2014;34(3):169-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  64. Altered IFN-gamma-mediated immunity and transcriptional expression patterns in N-Ethyl-N-nitrosourea-induced STAT4 mutants confer susceptibility to acute typhoid-like disease.
    Authors: Eva M, Yuki K, Dauphinee S, Schwartzentruber J, Pyzik M, Paquet M, Lathrop M, Majewski J, Vidal S, Malo D
    J Immunol, 2014;192(1):259-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  65. Hepatocyte growth factor limits autoimmune neuroinflammation via glucocorticoid-induced leucine zipper expression in dendritic cells.
    Authors: Benkhoucha M, Molnarfi N, Dunand-Sauthier I, Merkler D, Schneiter G, Bruscoli S, Riccardi C, Tabata Y, Funakoshi H, Nakamura T, Reith W, Santiago-Raber M, Lalive P
    J Immunol, 2014;193(6):2743-52.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  66. Dynamic Treg interactions with intratumoral APCs promote local CTL dysfunction.
    Authors: Bauer, Christia, Kim, Edward Y, Marangoni, Francesc, Carrizosa, Esteban, Claudio, Natalie, Mempel, Thorsten
    J Clin Invest, 2014;124(6):2425-40.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  67. Two separate defects affecting true naive or virtual memory T cell precursors combine to reduce naive T cell responses with aging.
    Authors: Renkema K, Li G, Wu A, Smithey M, Nikolich-Zugich J
    J Immunol, 2014;192(1):151-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  68. The metabolic checkpoint kinase mTOR is essential for IL-15 signaling during the development and activation of NK cells.
    Authors: Marcais A, Cherfils-Vicini J, Viant C, Degouve S, Viel S, Fenis A, Rabilloud J, Mayol K, Tavares A, Bienvenu J, Gangloff Y, Gilson E, Vivier E, Walzer T
    Nat Immunol, 2014;15(8):749-57.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  69. Parent-of-origin effects implicate epigenetic regulation of experimental autoimmune encephalomyelitis and identify imprinted Dlk1 as a novel risk gene.
    Authors: Stridh P, Ruhrmann S, Bergman P, Thessen Hedreul M, Flytzani S, Beyeen A, Gillett A, Krivosija N, Ockinger J, Ferguson-Smith A, Jagodic M
    PLoS Genet, 2014;10(3):e1004265.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  70. Ezh2 regulates transcriptional and posttranslational expression of T-bet and promotes Th1 cell responses mediating aplastic anemia in mice.
    Authors: Tong Q, He S, Xie F, Mochizuki K, Liu Y, Mochizuki I, Meng L, Sun H, Zhang Y, Guo Y, Hexner E, Zhang Y
    J Immunol, 2014;192(11):5012-22.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  71. Distinct requirements for activation of NKT and NK cells during viral infection.
    Authors: Tyznik A, Verma S, Wang Q, Kronenberg M, Benedict C
    J Immunol, 2014;192(8):3676-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  72. Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses.
    Authors: Chiba, Shiho, Ikushima, Hiroaki, Ueki, Hiroshi, Yanai, Hideyuki, Kimura, Yoshitak, Hangai, Sho, Nishio, Junko, Negishi, Hideo, Tamura, Tomohiko, Saijo, Shinobu, Iwakura, Yoichiro, Taniguchi, Tadatsug
    Elife, 2014;3(0):e04177.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  73. Dual-specificity phosphatase 14 (DUSP14/MKP6) negatively regulates TCR signaling by inhibiting TAB1 activation.
    Authors: Yang C, Li J, Chiu L, Lan J, Chen D, Chuang H, Huang C, Tan T
    J Immunol, 2014;192(4):1547-57.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  74. Effect of methamphetamine on the microglial damage: role of potassium channel Kv1.3.
    Authors: Wang J, Qian W, Liu J, Zhao J, Yu P, Jiang L, Zhou J, Gao R, Xiao H
    PLoS ONE, 2014;9(2):e88642.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  75. MicroRNA-29b modulates innate and antigen-specific immune responses in mouse models of autoimmunity.
    Authors: Salama A, Fichou N, Allard M, Dubreil L, De Beaurepaire L, Viel A, Jegou D, Bosch S, Bach J
    PLoS ONE, 2014;9(9):e106153.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  76. NADPH oxidase modifies patterns of MHC class II-restricted epitopic repertoires through redox control of antigen processing.
    Authors: Allan E, Tailor P, Balce D, Pirzadeh P, McKenna N, Renaux B, Warren A, Jirik F, Yates R
    J Immunol, 2014;192(11):4989-5001.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  77. Aptamer-targeted inhibition of mTOR in T cells enhances antitumor immunity.
    Authors: Berezhnoy, Alexey, Castro, Iris, Levay, Agata, Malek, Thomas R, Gilboa, Eli
    J Clin Invest, 2014;124(1):188-97.
    Species: Mouse
    Sample Type: Whole Cells
    Application: celll culture
  78. Essential role of CD11a in CD8+ T-cell accumulation and activation in adipose tissue.
    Authors: Jiang E, Perrard X, Yang D, Khan I, Perrard J, Smith C, Ballantyne C, Wu H
    Arterioscler Thromb Vasc Biol, 2014;34(1):34-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  79. Polymicrobial sepsis alters antigen-dependent and -independent memory CD8 T cell functions.
    Authors: Duong S, Condotta S, Rai D, Martin M, Griffith T, Badovinac V
    J Immunol, 2014;192(8):3618-25.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  80. Autocrine interleukin-6 drives skin-derived mesenchymal stem cell trafficking via regulating voltage-gated Ca(2+) channels.
    Authors: Ke F, Zhang L, Liu Z, Liu J, Yan S, Xu Z, Bai J, Zhu H, Lou F, Wang H, Shi Y, Jiang Y, Su B, Wang H
    Stem Cells, 2014;32(10):2799-810.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  81. Suppression of Th1-mediated autoimmunity by embryonic stem cell-derived dendritic cells.
    Authors: Ikeda T, Hirata S, Takamatsu K, Haruta M, Tsukamoto H, Ito T, Uchino M, Ando Y, Nagafuchi S, Nishimura Y, Senju S
    PLoS ONE, 2014;9(12):e115198.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  82. Lnk/Sh2b3 controls the production and function of dendritic cells and regulates the induction of IFN-gamma-producing T cells.
    Authors: Mori T, Iwasaki Y, Seki Y, Iseki M, Katayama H, Yamamoto K, Takatsu K, Takaki S
    J Immunol, 2014;193(4):1728-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  83. Astrocytes modulate the polarization of CD4+ T cells to Th1 cells.
    Authors: Beurel E, Harrington L, Buchser W, Lemmon V, Jope R
    PLoS ONE, 2014;9(1):e86257.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  84. Role of Blimp-1 in programing Th effector cells into IL-10 producers.
    Authors: Neumann C, Heinrich F, Neumann K, Junghans V, Mashreghi M, Ahlers J, Janke M, Rudolph C, Mockel-Tenbrinck N, Kuhl A, Heimesaat M, Esser C, Im S, Radbruch A, Rutz S, Scheffold A
    J Exp Med, 2014;211(9):1807-19.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  85. Cell-selective knockout and 3D confocal image analysis reveals separate roles for astrocyte-and endothelial-derived CCL2 in neuroinflammation.
    Authors: Paul D, Ge S, Lemire Y, Jellison E, Serwanski D, Ruddle N, Pachter J
    J Neuroinflammation, 2014;11(0):10.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  86. CDK8 kinase phosphorylates transcription factor STAT1 to selectively regulate the interferon response.
    Authors: Bancerek J, Poss Z, Steinparzer I, Sedlyarov V, Pfaffenwimmer T, Mikulic I, Dolken L, Strobl B, Muller M, Taatjes D, Kovarik P
    Immunity, 2013;38(2):250-62.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  87. Regulatory B cells suppress imiquimod-induced, psoriasis-like skin inflammation.
    Authors: Yanaba K, Kamata M, Ishiura N, Shibata S, Asano Y, Tada Y, Sugaya M, Kadono T, Tedder T, Sato S
    J Leukoc Biol, 2013;94(4):563-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  88. Regulation and migratory role of P-selectin ligands during intestinal inflammation.
    Authors: Hoffmann U, Pink M, Lauer U, Heimesaat M, Winsauer C, Kruglov A, Schlawe K, Leichsenring C, Liesenfeld O, Hamann A, Syrbe U
    PLoS ONE, 2013;8(4):e62055.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  89. IL-21 promotes CD8+ CTL activity via the transcription factor T-bet.
    Authors: Sutherland A, Joller N, Michaud M, Liu S, Kuchroo V, Grusby M
    J Immunol, 2013;190(8):3977-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  90. IL-27 receptor signalling restricts the formation of pathogenic, terminally differentiated Th1 cells during malaria infection by repressing IL-12 dependent signals.
    Authors: Villegas-Mendez A, de Souza J, Lavelle S, Gwyer Findlay E, Shaw T, Van Rooijen N, Saris C, Hunter C, Riley E, Couper K
    PLoS Pathog, 2013;9(4):e1003293.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Bioassay
  91. Regulation of Foxp3+ inducible regulatory T cell stability by SOCS2.
    Authors: Knosp C, Schiering C, Spence S, Carroll H, Nel H, Osbourn M, Jackson R, Lyubomska O, Malissen B, Ingram R, Fitzgerald D, Powrie F, Fallon P, Johnston J, Kissenpfennig A
    J Immunol, 2013;190(7):3235-45.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  92. T helper type 17 cells contribute to anti-tumour immunity and promote the recruitment of T helper type 1 cells to the tumour.
    Authors: Nunez S, Saez J, Fernandez D, Flores-Santibanez F, Alvarez K, Tejon G, Ruiz P, Maldonado P, Hidalgo Y, Manriquez V, Bono M, Rosemblatt M, Sauma D
    Immunology, 2013;139(1):61-71.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  93. Regulation of Th1 cells and experimental autoimmune encephalomyelitis by glycogen synthase kinase-3.
    Authors: Beurel E, Kaidanovich-Beilin O, Yeh W, Song L, Palomo V, Michalek S, Woodgett J, Harrington L, Eldar-Finkelman H, Martinez A, Jope R
    J Immunol, 2013;190(10):5000-11.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  94. Galectin-9 functionally impairs natural killer cells in humans and mice.
    Authors: Golden-Mason L, McMahan R, Strong M, Reisdorph R, Mahaffey S, Palmer B, Cheng L, Kulesza C, Hirashima M, Niki T, Rosen H
    J Virol, 2013;87(9):4835-45.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  95. Lymphoid-tissue stromal cells coordinate innate defense to cytomegalovirus.
    Authors: Verma S, Wang Q, Chodaczek G, Benedict C
    J Virol, 2013;87(11):6201-10.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Standard
  96. Abundant stage-dependent Ly49E expression by liver NK cells is not essential for their differentiation and function.
    Authors: Filtjens, Jessica, Taveirne, Sylvie, Van Acker, Aline, Van Ammel, Els, Vanhees, Mandy, Kerre, Tessa, Taghon, Tom, Vandekerckhove, Bart, Plum, Jean, Leclercq, Georges
    J Leukoc Biol, 2013;93(5):699-711.
  97. Differentially imprinted innate immunity by mucosal boost vaccination determines antituberculosis immune protective outcomes, independent of T-cell immunity.
    Authors: Jeyanathan M, Damjanovic D, Shaler C, Lai R, Wortzman M, Yin C, Zganiacz A, Lichty B, Xing Z
    Mucosal Immunol, 2013;6(3):612-25.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  98. p53 controls autoimmune arthritis via STAT-mediated regulation of the Th17 cell/Treg cell balance in mice.
    Authors: Park J, Lim M, Cho M, Ryu J, Moon Y, Jhun J, Byun J, Kim E, Hwang S, Ju J, Kwok S, Kim H
    Arthritis Rheum, 2013;65(4):949-59.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  99. TNF-like ligand 1A (TL1A) gene knockout leads to ameliorated collagen-induced arthritis in mice: implication of TL1A in humoral immune responses.
    Authors: Wang X, Hu Y, Charpentier T, Lamarre A, Qi S, Wu J, Luo H
    J Immunol, 2013;191(11):5420-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: cell culture
  100. IL-27 acts on DCs to suppress the T cell response and autoimmunity by inducing expression of the immunoregulatory molecule CD39.
    Authors: Mascanfroni, Ivan D, Yeste, Ada, Vieira, Silvio M, Burns, Evan J, Patel, Bonny, Sloma, Ido, Wu, Yan, Mayo, Lior, Ben-Hamo, Rotem, Efroni, Sol, Kuchroo, Vijay K, Robson, Simon C, Quintana, Francisc
    Nat Immunol, 2013;14(10):1054-63.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  101. Robust tumor immunity to melanoma mediated by interleukin-9-producing T cells.
    Nat. Med., 2012;1248(0):18.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  102. Chemotherapy-triggered cathepsin B release in myeloid-derived suppressor cells activates the Nlrp3 inflammasome and promotes tumor growth.
    Authors: Bruchard M, Mignot G, Derangere V, Chalmin F, Chevriaux A, Vegran F, Boireau W, Simon B, Ryffel B, Connat J, Kanellopoulos J, Martin F, Rebe C, Apetoh L, Ghiringhelli F
    Nat Med, 2012;19(1):57-64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  103. Regulation of innate CD8+ T-cell activation mediated by cytokines.
    Proc Natl Acad Sci U S A, 2012;109(25):9971-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  104. An epigenetic silencing pathway controlling T helper 2 cell lineage commitment.
    Authors: Allan RS, Zueva E, Cammas F, Schreiber HA, Masson V, Belz GT, Roche D, Maison C, Quivy JP, Almouzni G, Amigorena S
    Nature, 2012;487(7406):249-53.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  105. NFIL3/E4BP4 controls type 2 T helper cell cytokine expression.
    Authors: Kashiwada M, Cassel SL, Colgan JD
    EMBO J., 2011;30(10):2071-82.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  106. The effect of conditional EFNB1 deletion in the T cell compartment on T cell development and function.
    Authors: Jin W, Qi S, Luo H
    BMC Immunol., 2011;12(0):68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  107. Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens.
    Authors: DePaolo RW, Abadie V, Tang F
    Nature, 2011;471(7337):220-4.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  108. Cell adhesion molecules regulate fibrotic process via Th1/Th2/Th17 cell balance in a bleomycin-induced scleroderma model.
    Authors: Yoshizaki A, Yanaba K, Iwata Y
    J. Immunol., 2010;185(4):2502-15.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  109. TIM-3: a novel regulatory molecule of alloimmune activation.
    Authors: Boenisch O, D'Addio F, Watanabe T, Elyaman W, Magee CN, Yeung MY, Padera RF, Rodig SJ, Murayama T, Tanaka K, Yuan X, Ueno T, Jurisch A, Mfarrej B, Akiba H, Yagita H, Najafian N
    J. Immunol., 2010;185(10):5806-19.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  110. Crucial role of interleukin-7 in T helper type 17 survival and expansion in autoimmune disease.
    Authors: Liu X, Leung S, Wang C, Tan Z, Wang J, Guo TB, Fang L, Zhao Y, Wan B, Qin X, Lu L, Li R, Pan H, Song M, Liu A, Hong J, Lu H, Zhang JZ
    Nat. Med., 2010;16(2):191-7.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  111. Self-specific CD8+ T cells maintain a semi-naive state following lymphopenia-induced proliferation.
    Authors: Johnson LD, Jameson SC
    J. Immunol., 2010;184(10):5604-11.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  112. Dissociated expression of natural killer 1.1 and T-cell receptor by invariant natural killer T cells after interleukin-12 receptor and T-cell receptor signalling.
    Authors: Emoto M, Shimizu T, Koike H, Yoshizawa I, Hurwitz R, Kaufmann SH, Emoto Y
    Immunology, 2010;129(1):62-74.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  113. Negative regulation of autoimmune demyelination by the inhibitory receptor CLM-1.
    Authors: Xi H, Katschke KJ, Helmy KY, Wark PA, Kljavin N, Clark H, Eastham-Anderson J, Shek T, Roose-Girma M, Ghilardi N, van Lookeren Campagne M
    J. Exp. Med., 2010;207(1):7-16, S1-5.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  114. TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.
    Authors: Perrot I, Deauvieau F, Massacrier C
    J. Immunol., 2010;185(4):2080-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  115. T cell Ig domain and mucin domain 1 engagement on invariant NKT cells in the presence of TCR stimulation enhances IL-4 production but inhibits IFN-gamma production.
    Authors: Kim HS, Kim HS, Lee CW, Chung DH
    J. Immunol., 2010;184(8):4095-106.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  116. Interleukin-1 family cytokines as mucosal vaccine adjuvants for induction of protective immunity against influenza virus.
    Authors: Kayamuro H, Yoshioka Y, Abe Y, Arita S, Katayama K, Nomura T, Yoshikawa T, Kubota-Koketsu R, Ikuta K, Okamoto S, Mori Y, Kunisawa J, Kiyono H, Itoh N, Nagano K, Kamada H, Tsutsumi Y, Tsunoda S
    J. Virol., 2010;84(24):12703-12.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  117. The pro-Th2 cytokine IL-33 directly interacts with invariant NKT and NK cells to induce IFN-gamma production.
    Authors: Bourgeois E, Van LP, Samson M, Diem S, Barra A, Roga S, Gombert JM, Schneider E, Dy M, Gourdy P, Girard JP, Herbelin A
    Eur. J. Immunol., 2009;39(4):1046-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  118. Thymic regulation of autoimmune disease by accelerated differentiation of Foxp3+ regulatory T cells through IL-7 signaling pathway.
    Authors: Chen X, Fang L, Song S, Guo TB, Liu A, Zhang JZ
    J. Immunol., 2009;183(10):6135-44.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  119. Professional memory CD4+ T lymphocytes preferentially reside and rest in the bone marrow.
    Authors: Tokoyoda K, Zehentmeier S, Hegazy AN, Albrecht I, Grun JR, Lohning M, Radbruch A
    Immunity, 2009;30(5):721-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  120. Capsular polysaccharide and the O-specific antigen impede antibody binding: a potential obstacle for the successful development of an extraintestinal pathogenic Escherichia coli vaccine.
    Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Cope JJ
    Vaccine, 2009;27(3):388-95.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  121. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  122. The induction of antibody production by IL-6 is indirectly mediated by IL-21 produced by CD4+ T cells.
    Authors: Dienz O, Eaton SM, Bond JP, Neveu W, Moquin D, Noubade R, Briso EM, Charland C, Leonard WJ, Ciliberto G, Teuscher C, Haynes L, Rincon M
    J. Exp. Med., 2009;206(1):69-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  123. Expression of bioactive single-chain murine IL-12 in transgenic plants.
    Authors: Liu J, Dolan MC, Reidy M, Cramer CL
    J. Interferon Cytokine Res., 2009;28(0):381-92.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  124. Effects of Signal 3 during CD8 T cell priming: Bystander production of IL-12 enhances effector T cell expansion but promotes terminal differentiation.
    Authors: Cui W, Joshi NS, Jiang A, Kaech SM
    Vaccine, 2009;27(15):2177-87.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  125. Activation of the NLRP3 inflammasome in dendritic cells induces IL-1beta-dependent adaptive immunity against tumors.
    Authors: Ghiringhelli F, Apetoh L, Tesniere A, Aymeric L, Ma Y, Ortiz C, Vermaelen K, Panaretakis T, Mignot G, Ullrich E, Perfettini JL, Schlemmer F, Tasdemir E, Uhl M, Genin P, Civas A, Ryffel B, Kanellopoulos J, Tschopp J, Andre F, Lidereau R, McLaughlin NM, Haynes NM, Smyth MJ, Kroemer G, Zitvogel L
    Nat. Med., 2009;15(10):1170-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  126. Interferon-gamma limits Th1 lymphocyte adhesion to inflamed endothelium: a nitric oxide regulatory feedback mechanism.
    Authors: Norman MU, Zbytnuik L, Kubes P
    Eur. J. Immunol., 2008;38(5):1368-80.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  127. CXCL12 (SDF-1alpha) suppresses ongoing experimental autoimmune encephalomyelitis by selecting antigen-specific regulatory T cells.
    Authors: Meiron M, Zohar Y, Anunu R, Wildbaum G, Karin N
    J. Exp. Med., 2008;205(11):2643-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  128. Redox regulation of interleukin-4 signaling.
    Authors: Sharma P, Chakraborty R, Wang L, Min B, Tremblay ML, Kawahara T, Lambeth JD, Haque SJ
    Immunity, 2008;29(4):551-64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  129. Induction of natural killer cell-dependent antitumor immunity by the Autographa californica multiple nuclear polyhedrosis virus.
    Authors: Kitajima M, Abe T, Miyano-Kurosaki N, Taniguchi M, Nakayama T, Takaku H
    Mol. Ther., 2007;16(2):261-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  130. IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice.
    Authors: Smith E, Zarbock A, Stark MA, Burcin TL, Bruce AC, Foley P, Ley K
    J. Immunol., 2007;179(12):8274-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  131. T helper 1 cells stimulated with ovalbumin and IL-18 induce airway hyperresponsiveness and lung fibrosis by IFN-gamma and IL-13 production.
    Authors: Hayashi N, Yoshimoto T, Izuhara K, Matsui K, Tanaka T, Nakanishi K
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(37):14765-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  132. Decay accelerating factor can control T cell differentiation into IFN-gamma-producing effector cells via regulating local C5a-induced IL-12 production.
    Authors: Lalli PN, Strainic MG, Lin F, Medof ME, Heeger PS
    J. Immunol., 2007;179(9):5793-802.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  133. T-bet inhibits both TH2 cell-mediated eosinophil recruitment and TH17 cell-mediated neutrophil recruitment into the airways.
    Authors: Fujiwara M, Hirose K, Kagami S, Takatori H, Wakashin H, Tamachi T, Watanabe N, Saito Y, Iwamoto I, Nakajima H
    J. Allergy Clin. Immunol., 2007;119(3):662-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  134. Intranasal interleukin-12 therapy inhibits Mycoplasma pneumoniae clearance and sustains airway obstruction in murine pneumonia.
    Authors: Salvatore CM, Fonseca-Aten M, Katz-Gaynor K, Gomez AM, Hardy RD
    Infect. Immun., 2007;76(2):732-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  135. The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Th1 cells in vitro and in delayed-type hypersensitivity reactions in vivo.
    Authors: Alcaide P, King SL, Dimitroff CJ, Lim YC, Fuhlbrigge RC, Luscinskas FW
    J. Invest. Dermatol., 2007;127(8):1964-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  136. Suppression of autoimmune inflammation of the central nervous system by interleukin 10 secreted by interleukin 27-stimulated T cells.
    Authors: Fitzgerald DC, Zhang GX, El-Behi M, Fonseca-Kelly Z, Li H, Yu S, Saris CJ, Gran B, Ciric B, Rostami A
    Nat. Immunol., 2007;8(12):1372-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  137. Involvement of mast cells in IL-12/23 p40 production is essential for survival from polymicrobial infections.
    Authors: Nakano N, Nishiyama C, Kanada S, Niwa Y, Shimokawa N, Ushio H, Nishiyama M, Okumura K, Ogawa H
    Blood, 2007;109(11):4846-55.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  138. Cytopiloyne, a novel polyacetylenic glucoside from Bidens pilosa, functions as a T helper cell modulator.
    Authors: Chiang YM, Chang CL, Chang SL, Yang WC, Shyur LF
    J Ethnopharmacol, 2007;110(3):532-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  139. Overexpression of suppressor of cytokine signaling-5 in T cells augments innate immunity during septic peritonitis.
    Authors: Watanabe H, Kubo M, Numata K, Takagi K, Mizuta H, Okada S, Ito T, Matsukawa A
    J. Immunol., 2006;177(12):8650-7.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  140. Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
    Authors: Wen H, Hogaboam CM, Gauldie J, Kunkel SL
    Am. J. Pathol., 2006;168(6):1940-50.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  141. Human complete Stat-1 deficiency is associated with defective type I and II IFN responses in vitro but immunity to some low virulence viruses in vivo.
    Authors: Chapgier A, Wynn RF, Jouanguy E, Filipe-Santos O, Zhang S, Feinberg J, Hawkins K, Casanova JL, Arkwright PD
    J. Immunol., 2006;176(8):5078-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  142. Exogenous IL-12 suppresses experimental autoimmune encephalomyelitis (EAE) by tuning IL-10 and IL-5 levels in an IFN-gamma-dependent way.
    Authors: Berghmans N, Dillen C, Heremans H
    J. Neuroimmunol., 2006;176(1):63-75.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  143. Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.
    Authors: An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
    J Ethnopharmacol, 2006;106(3):418-24.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  144. Activin A functions as a Th2 cytokine in the promotion of the alternative activation of macrophages.
    Authors: Ogawa K, Funaba M, Chen Y, Tsujimoto M
    J. Immunol., 2006;177(10):6787-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  145. Increased sensitivity to Salmonella enterica serovar Typhimurium infection in mice undergoing withdrawal from morphine is associated with suppression of interleukin-12.
    Authors: Feng P, Wilson QM, Meissler JJ, Adler MW, Eisenstein TK
    Infect. Immun., 2005;73(12):7953-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  146. Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages.
    Authors: Harrington, Laurie E, Hatton, Robin D, Mangan, Paul R, Turner, Henriett, Murphy, Theresa, Murphy, Kenneth, Weaver, Casey T
    Nat Immunol, 2005;6(11):1123-32.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  147. Sequential ELISA to profile multiple cytokines from small volumes.
    Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
    J. Immunol. Methods, 2005;302(1):172-81.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  148. Pivotal role of endogenous tachykinins and the NK1 receptor in mediating leukocyte accumulation, in the absence of oedema formation, in response to TNFalpha in the cutaneous microvasculature.
    Authors: Costa SK, Yshii LM, Poston RN, Muscara MN, Brain SD
    J. Neuroimmunol., 2005;171(1):99-109.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  149. TCR vaccines against a murine T cell lymphoma: a primary role for antibodies of the IgG2c class in tumor protection.
    Authors: Lambert SL, Okada CY, Levy R
    J. Immunol., 2004;172(2):929-36.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  150. 17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
    Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
    Blood, 2004;104(5):1404-10.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  151. Control of Leishmania major in the absence of Tyk2 kinase.
    Authors: Schleicher U, Mattner J, Blos M, Schindler H, Rollinghoff M, Karaghiosoff M, Muller M, Werner-Felmayer G, Bogdan C
    Eur. J. Immunol., 2004;34(2):519-29.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  152. IL-12 p40 homodimer-dependent macrophage chemotaxis and respiratory viral inflammation are mediated through IL-12 receptor beta 1.
    Authors: Russell TD, Yan Q, Fan G, Khalifah AP, Bishop DK, Brody SL, Walter MJ
    J. Immunol., 2003;171(12):6866-74.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  153. Intracellular thiols contribute to Th2 function via a positive role in IL-4 production.
    Authors: Monick MM, Samavati L, Butler NS, Mohning M, Powers LS, Yarovinsky T, Spitz DR, Hunninghake GW
    J. Immunol., 2003;171(10):5107-15.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  154. STAT4 serine phosphorylation is critical for IL-12-induced IFN-gamma production but not for cell proliferation.
    Authors: O&amp;amp;apos;shea JJ
    Proc. Natl. Acad. Sci. U.S.A., 2002;99(19):12281-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  155. A receptor for the heterodimeric cytokine IL-23 is composed of IL-12Rbeta1 and a novel cytokine receptor subunit, IL-23R.
    Authors: Parham C, Chirica M, Timans J, Vaisberg E, Travis M, Cheung J, Pflanz S, Zhang R, Singh KP, Vega F, To W, Wagner J, O&amp;amp;apos;Farrell AM, McClanahan T, Zurawski S, Hannum C, Gorman D, Rennick DM, Kastelein RA, de Waal Malefyt R, Moore KW
    J. Immunol., 2002;168(11):5699-708.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  156. Cutting Edge: MicroRNA-223 Regulates Myeloid Dendritic Cell-Driven Th17 Responses in Experimental Autoimmune Encephalomyelitis.
    Authors: Ifergan I, Chen S, Zhang B, Miller S
    J Immunol, 0;196(4):1455-9.
    Species: Mouse
    Sample Type: In Vivo
Expand to show all 156 Citations
Average Rating: 4.7 (Based on 3 reviews)

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We have 3 reviews tested in 3 applications: Immunoassay Standard, CD4+ T cell polarization toward Th1, In vitro bioactivity in cell culture.
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Immunoassay Standard Recombinant Mouse IL-12 Protein 419-ML
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Excellent
 Immunoassay Standard Anonymous 11/27/2017
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Immunoassay Standard Recombinant Mouse IL-12 Protein 419-ML
Immunoassay Standard: Recombinant Mouse IL-12 Protein [419-ML]

Summary

ApplicationImmunoassay Standard

Other Experimental Details

Other Experimental DetailsThis protein was used as the calibrator in an ELISA using MAB419 and BAF419 as the matched pair antibodies.
CD4+ T cell polarization toward Th1 Recombinant Mouse IL-12 Protein 419-ML
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Excellent
 CD4+ T cell polarization toward Th1 Anonymous 08/24/2017
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CD4+ T cell polarization toward Th1 Recombinant Mouse IL-12 Protein 419-ML
CD4+ T cell polarization toward Th1: Recombinant Mouse IL-12 Protein [419-ML]

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ApplicationCD4+ T cell polarization toward Th1
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 In vitro bioactivity in cell culture Anonymous 01/21/2016
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Summary

ApplicationIn vitro bioactivity in cell culture

FAQs

  1. What is the extra band observed when running Recombinant Mouse IL-12 Protein (Catalog # 419-ML) in Western blot?

    • Recombinant mouse IL‑12 preparations were proteolytically cleaved between residues G158 and E159 of the mature p35 subunit. Thus, under reducing conditions, three bands representing the p40 subunit, the p35 R1 ‑ G158 peptide and the p35 E159 - A193 peptide can be observed in SDS-PAGE.

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