Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
4.98 pg/mL
Assay Range
39.1 - 2,500 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat Angiopoietin-2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse/Rat Angiopoietin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat Angiopoietin-2 in cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant mouse Angiopoietin-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural Angiopoietin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural Angiopoietin-2.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 119 | 290 | 831 | 116 | 283 | 841 |
| Standard Deviation | 3.27 | 9.05 | 22.2 | 9.84 | 23.2 | 63.1 |
| CV% | 2.7 | 3.1 | 2.7 | 8.5 | 8.2 | 7.5 |
Recovery
The recovery of Angiopoietin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 114 | 108-120 |
| EDTA Plasma (n=2) | 105 | 101-110 |
| Heparin Plasma (n=2) | 107 | 103-111 |
| Serum (n=2) | 113 | 109-117 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-2
Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
Entrez Gene IDs:
285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)
Alternate Names:
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Citations for Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
17
Citations: Showing 1 - 10
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Detrimental effects of PCSK9 loss-of-function in the pediatric host response to sepsis are mediated through independent influence on Angiopoietin-1
Authors: MR Atreya, NZ Cvijanovic, JC Fitzgerald, SL Weiss, MT Bigham, PN Jain, AJ Schwarz, R Lutfi, J Nowak, GL Allen, NJ Thomas, JR Grunwell, T Baines, M Quasney, B Haileselas, MN Alder, P Lahni, S Ripberger, A Ekunwe, KR Campbell, KR Walley, SW Standage
Research square, 2023-02-03;0(0):.
Species: Mouse
Sample Types: Serum
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Angpt2/Tie2 autostimulatory loop controls tumorigenesis
Authors: NM Karabid, T Wiedemann, S Gulde, H Mohr, RC Segaran, J Geppert, M Rohm, G Vitale, G Gaudenzi, A Dicitore, DP Ankerst, Y Chen, R Braren, G Kaissis, F Schilling, M Schillmaie, G Eisenhofer, S Herzig, F Roncaroli, JB Honegger, NS Pellegata
Embo Molecular Medicine, 2022-03-10;0(0):e14364.
Species: Rat
Sample Types: Cell Culture Supernates
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The Angiopoietin-Tie2 axis contributes to placental vascular disruption and adverse birth outcomes in malaria in pregnancy
Authors: V Tran, AM Weckman, VM Crowley, LS Cahill, K Zhong, A Cabrera, RE Elphinston, V Pearce, M Madanitsa, L Kalilani-P, V Mwapasa, C Khairallah, AL Conroy, FO Ter Kuile, JG Sled, KC Kain
EBioMedicine, 2021-11-07;73(0):103683.
Species: Mouse
Sample Types: Plasma
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Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice
Authors: NV Bogatcheva, ME Coleman
Cells, 2021-03-24;10(4):.
Species: Mouse
Sample Types: BALF
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Inducible mechanisms of disease tolerance provide an alternative strategy of acquired immunity to malaria
Authors: W Nahrendorf, A Ivens, PJ Spence
Elife, 2021-03-23;10(0):.
Species: Mouse
Sample Types: Plasma
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Angiopoietin-2 is released during anaphylactic hypotension in anesthetized and unanesthetized rats
Authors: T Zhang, T Shibamoto, M Tanida, M Taniguchi, Y Kuda, W Yang, Y Kurata
PLoS ONE, 2020-11-17;15(11):e0242026.
Species: Rat
Sample Types: Plasma
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NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture
Authors: DC Cornelius, OK Travis, RW Tramel, M Borges-Rod, CH Baik, M Greer, CA Giachelli, GA Tardo, JM Williams
PLoS ONE, 2020-06-17;15(6):e0234039.
Species: Rat
Sample Types: Plasma
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Angiopoietin-2-integrin alpha5beta1 signaling enhances vascular fatty acid transport and prevents ectopic lipid-induced insulin resistance
Authors: H Bae, KY Hong, CK Lee, C Jang, SJ Lee, K Choe, S Offermanns, Y He, HJ Lee, GY Koh
Nat Commun, 2020-06-12;11(1):2980.
Species: Mouse
Sample Types: Serum
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The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye
Authors: A Wolf, M Herb, M Schramm, T Langmann
Nat Commun, 2020-06-01;11(1):2709.
Species: Mouse
Sample Types: Tissue Homogenates
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Angiopoietin-2 blockade ameliorates autoimmune neuroinflammation by inhibiting leukocyte recruitment into the CNS
Authors: Z Li, EA Korhonen, A Merlini, J Strauss, E Wihuri, H Nurmi, S Antila, J Paech, U Deutsch, B Engelhardt, S Chintharla, GY Koh, A Flügel, K Alitalo
J. Clin. Invest., 2020-04-01;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Effect of Icariside II and Metformin on Penile Erectile Function, Histological Structure, Mitochondrial Autophagy, Glucose-Lipid Metabolism, Angiotensin II and Sex Hormone in Type 2 Diabetic Rats With Erectile Dysfunction
Authors: J Zhang, S Li, S Zhang, Y Wang, S Jin, C Zhao, W Yang, Y Liu, G Kong
Sex Med, 2020-03-05;0(0):.
Species: Rat
Sample Types: Serum
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Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861
Authors: AR Pedrosa, N Bodrug, J Gomez-Escu, EP Carter, LE Reynolds, PN Georgiou, I Fernandez, DM Lees, V Kostourou, AN Alexopoulo, S Batista, B Tavora, B Serrels, M Parsons, T Iskratsch, KM Hodivala-D
Cancer Res., 2019-06-12;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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NLRP3 inflammasome activation in platelets in response to sepsis
Authors: DC Cornelius, CH Baik, OK Travis, DL White, CM Young, W Austin Pie, CA Shields, B Poudel, JM Williams
Physiol Rep, 2019-05-01;7(9):e14073.
Species: Rat
Sample Types: Plasma
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Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration
Authors: J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh
Sci Adv, 2019-02-13;5(2):eaau6732.
Species: Mouse
Sample Types: Vitreous Humor
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Endothelial pyruvate kinase M2 maintains vascular integrity
Authors: B Kim, C Jang, H Dharaneesw, J Li, M Bhide, S Yang, K Li, Z Arany
J. Clin. Invest., 2018-09-17;0(0):.
Species: Mouse
Sample Types: Plasma
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Synthetic oleanane triterpenoids enhance blood brain barrier integrity and improve survival in experimental cerebral malaria
Authors: VM Crowley, K Ayi, Z Lu, KT Liby, M Sporn, KC Kain
Malar. J., 2017-11-14;16(1):463.
Species: Mouse
Sample Types: Plasma
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Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
Authors: Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger M, Wu D, Min W
Nat Med, 2016-08-22;22(9):1033-1042.
Species: Mouse
Sample Types: Tissue Homogenates
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