Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Mouse/Rat Angiopoietin-2 ELISA Standard Curve
2 Images
Product Details
Citations (17)
Supplemental Products

Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
4.98 pg/mL
Assay Range
39.1 - 2,500 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Natural and recombinant mouse and rat Angiopoietin-2.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat Angiopoietin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat Angiopoietin-2 in cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant mouse Angiopoietin-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural Angiopoietin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural Angiopoietin-2.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 119 290 831 116 283 841
Standard Deviation 3.27 9.05 22.2 9.84 23.2 63.1
CV% 2.7 3.1 2.7 8.5 8.2 7.5


The recovery of Angiopoietin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 114 108-120
EDTA Plasma (n=2) 105 101-110
Heparin Plasma (n=2) 107 103-111
Serum (n=2) 113 109-117


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat Angiopoietin-2 ELISA Linearity

Scientific Data

Mouse/Rat Angiopoietin-2 ELISA Standard Curve

Product Datasheets

You must select a language.


Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Angiopoietin-2

Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.

Entrez Gene IDs:
285 (Human); 11601 (Mouse); 89805 (Rat); 396730 (Porcine); 101925495 (Cynomolgus Monkey)
Alternate Names:
AGPT2; ANG2; ANG-2; angiopoietin 2; Angiopoietin-2; angiopoietin-2a; angiopoietin-2B; angiopoitin 2; ANGPT2; Tie2-ligand
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Detrimental effects of PCSK9 loss-of-function in the pediatric host response to sepsis are mediated through independent influence on Angiopoietin-1
    Authors: MR Atreya, NZ Cvijanovic, JC Fitzgerald, SL Weiss, MT Bigham, PN Jain, AJ Schwarz, R Lutfi, J Nowak, GL Allen, NJ Thomas, JR Grunwell, T Baines, M Quasney, B Haileselas, MN Alder, P Lahni, S Ripberger, A Ekunwe, KR Campbell, KR Walley, SW Standage
    Research square, 2023-02-03;0(0):.
    Species: Mouse
    Sample Types: Serum
  2. Angpt2/Tie2 autostimulatory loop controls tumorigenesis
    Authors: NM Karabid, T Wiedemann, S Gulde, H Mohr, RC Segaran, J Geppert, M Rohm, G Vitale, G Gaudenzi, A Dicitore, DP Ankerst, Y Chen, R Braren, G Kaissis, F Schilling, M Schillmaie, G Eisenhofer, S Herzig, F Roncaroli, JB Honegger, NS Pellegata
    Embo Molecular Medicine, 2022-03-10;0(0):e14364.
    Species: Rat
    Sample Types: Cell Culture Supernates
  3. The Angiopoietin-Tie2 axis contributes to placental vascular disruption and adverse birth outcomes in malaria in pregnancy
    Authors: V Tran, AM Weckman, VM Crowley, LS Cahill, K Zhong, A Cabrera, RE Elphinston, V Pearce, M Madanitsa, L Kalilani-P, V Mwapasa, C Khairallah, AL Conroy, FO Ter Kuile, JG Sled, KC Kain
    EBioMedicine, 2021-11-07;73(0):103683.
    Species: Mouse
    Sample Types: Plasma
  4. Concentrated Secretome of Adipose Stromal Cells Limits Influenza A Virus-Induced Lung Injury in Mice
    Authors: NV Bogatcheva, ME Coleman
    Cells, 2021-03-24;10(4):.
    Species: Mouse
    Sample Types: BALF
  5. Inducible mechanisms of disease tolerance provide an alternative strategy of acquired immunity to malaria
    Authors: W Nahrendorf, A Ivens, PJ Spence
    Elife, 2021-03-23;10(0):.
    Species: Mouse
    Sample Types: Plasma
  6. Angiopoietin-2 is released during anaphylactic hypotension in anesthetized and unanesthetized rats
    Authors: T Zhang, T Shibamoto, M Tanida, M Taniguchi, Y Kuda, W Yang, Y Kurata
    PLoS ONE, 2020-11-17;15(11):e0242026.
    Species: Rat
    Sample Types: Plasma
  7. NLRP3 inflammasome inhibition attenuates sepsis-induced platelet activation and prevents multi-organ injury in cecal-ligation puncture
    Authors: DC Cornelius, OK Travis, RW Tramel, M Borges-Rod, CH Baik, M Greer, CA Giachelli, GA Tardo, JM Williams
    PLoS ONE, 2020-06-17;15(6):e0234039.
    Species: Rat
    Sample Types: Plasma
  8. Angiopoietin-2-integrin alpha5beta1 signaling enhances vascular fatty acid transport and prevents ectopic lipid-induced insulin resistance
    Authors: H Bae, KY Hong, CK Lee, C Jang, SJ Lee, K Choe, S Offermanns, Y He, HJ Lee, GY Koh
    Nat Commun, 2020-06-12;11(1):2980.
    Species: Mouse
    Sample Types: Serum
  9. The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye
    Authors: A Wolf, M Herb, M Schramm, T Langmann
    Nat Commun, 2020-06-01;11(1):2709.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Angiopoietin-2 blockade ameliorates autoimmune neuroinflammation by inhibiting leukocyte recruitment into the CNS
    Authors: Z Li, EA Korhonen, A Merlini, J Strauss, E Wihuri, H Nurmi, S Antila, J Paech, U Deutsch, B Engelhardt, S Chintharla, GY Koh, A Flügel, K Alitalo
    J. Clin. Invest., 2020-04-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Effect of Icariside II and Metformin on Penile Erectile Function, Histological Structure, Mitochondrial Autophagy, Glucose-Lipid Metabolism, Angiotensin II and Sex Hormone in Type 2 Diabetic Rats With Erectile Dysfunction
    Authors: J Zhang, S Li, S Zhang, Y Wang, S Jin, C Zhao, W Yang, Y Liu, G Kong
    Sex Med, 2020-03-05;0(0):.
    Species: Rat
    Sample Types: Serum
  12. Tumor angiogenesis is differentially regulated by phosphorylation of endothelial cell focal adhesion kinase tyrosines-397 and -861
    Authors: AR Pedrosa, N Bodrug, J Gomez-Escu, EP Carter, LE Reynolds, PN Georgiou, I Fernandez, DM Lees, V Kostourou, AN Alexopoulo, S Batista, B Tavora, B Serrels, M Parsons, T Iskratsch, KM Hodivala-D
    Cancer Res., 2019-06-12;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. NLRP3 inflammasome activation in platelets in response to sepsis
    Authors: DC Cornelius, CH Baik, OK Travis, DL White, CM Young, W Austin Pie, CA Shields, B Poudel, JM Williams
    Physiol Rep, 2019-05-01;7(9):e14073.
    Species: Rat
    Sample Types: Plasma
  14. Tie2 activation promotes choriocapillary regeneration for alleviating neovascular age-related macular degeneration
    Authors: J Kim, JR Park, J Choi, I Park, Y Hwang, H Bae, Y Kim, W Choi, JM Yang, S Han, TY Chung, P Kim, Y Kubota, HG Augustin, WY Oh, GY Koh
    Sci Adv, 2019-02-13;5(2):eaau6732.
    Species: Mouse
    Sample Types: Vitreous Humor
  15. Endothelial pyruvate kinase M2 maintains vascular integrity
    Authors: B Kim, C Jang, H Dharaneesw, J Li, M Bhide, S Yang, K Li, Z Arany
    J. Clin. Invest., 2018-09-17;0(0):.
    Species: Mouse
    Sample Types: Plasma
  16. Synthetic oleanane triterpenoids enhance blood brain barrier integrity and improve survival in experimental cerebral malaria
    Authors: VM Crowley, K Ayi, Z Lu, KT Liby, M Sporn, KC Kain
    Malar. J., 2017-11-14;16(1):463.
    Species: Mouse
    Sample Types: Plasma
  17. Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.
    Authors: Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger M, Wu D, Min W
    Nat Med, 2016-08-22;22(9):1033-1042.
    Species: Mouse
    Sample Types: Tissue Homogenates


No product specific FAQs exist for this product, however you may

View all ELISA FAQs

Supplemental ELISA Products

Reviews for Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit

There are currently no reviews for this product. Be the first to review Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit and earn rewards!

Have you used Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review