Mouse/Rat DARC PE-conjugated Antibody

(1 citations)   
  • Species Reactivity
    Mouse, Rat
  • Specificity
    Detects mouse and rat DARC in Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse DARC
    Met1-Pro61, Ala115-Cys127, Ser186-Lys205, Tyr264-Asn285
    Accession # NP_034175
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of DARC in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse TER‑119 APC‑conjugated Monoclonal Antibody (Catalog # FAB1125A) and either (A) Sheep Anti-Mouse/Rat DARC PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.
Detection of DARC in HEK293 Human Cell Line Transfected with Mouse DARC and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse DARC and eGFP was stained with and either (A) Sheep Anti-Mouse/Rat DARC PE‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695P) or (B) Normal Sheep IgG Phycoerythrin Control (Catalog # IC016P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DARC

DARC (Duffy Antigen Receptor for Chemokines; also CD234) is a 40-46 kDa glycoprotein member of the Duffy family of silent heptahelical chemokine receptors. It is expressed in liver and on select neurons, erythrocytes and the endothelium of postcapillary venules. Unlike traditional chemokine receptors, DARC cannot signal through G-proteins as it lacks a DRYLAIVHA cytoplasmic motif. DARC has three potential functions: first, it binds circulating inflammatory-type chemokines, serving as a repository for future chemokine release; second, it acts as a vehicle by which chemokines are transported from the abluminal to the luminal side of endothelium; and third, it complexes with signal-transducing chemokine receptors to create a nonsignaling heterodimer. Mouse DARC is 334 amino acids (aa) in length. It contains a 62 aa N-terminal extracellular region, and a 28 aa C-terminal cytoplasmic tail. There is one potential splice variant that shows a 42 aa substitution for aa 133-334. Collectively, over the four extracellular domains (aa 1-62, 115-127, 186-205, 264-285), mouse DARC shares 52% and 75% aa identity with human and rat DARC, respectively.

  • Long Name:
    Duffy Antigen, Receptor For Chemokines
  • Entrez Gene IDs:
    2532 (Human); 13349 (Mouse)
  • Alternate Names:
    ACKR1; CCBP1; CD234 antigen; CD234; DARC; Dfy; Duffy Antigen; Duffy antigen/chemokine receptor; Duffy blood group antigen; Duffy blood group, chemokine receptor; Fy Antigen; Fy glycoprotein; FYDuffy blood group; Glycoprotein D; GPD; GPDWBCQ1; GpFy; Plasmodium vivax receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Atypical chemokine receptor 1 on nucleated erythroid cells regulates hematopoiesis
    Authors: J Duchene, I Novitzky-B, A Thiriot, M Casanova-A, M Bianchini, SL Etheridge, E Hub, K Nitz, K Artinger, K Eller, J Caamaño, T Rülicke, P Moss, RTA Megens, UH von Andria, A Hidalgo, C Weber, A Rot
    Nat. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Sheep IgG PE-conjugated Antibody

Ctrl IC016P  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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