Mouse TER-119 APC-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of TER‑119 in Mouse BALB/c Bone Marrow Cells by Flow Cytometry. Mouse BALB/c bone marrow cells were stained with Rat Anti-Mouse CD45 Phycoerythrin-conjugated Monoclonal Antibody (Catalog # FAB114P) and either (A) Rat Anti-Mouse TER-119 APC-conjugated Monoclonal Antibody (Catalog # FAB1125A) or (B) Rat IgG2BAllophycocyanin Isotype Control (Catalog # IC013A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3).
- Kina, T. et al. (2000) Br. J. Haematol. 109:280.
- Ikuta, K. et al. (1990) Cell 62:863.
- Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5
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