Follistatin-Like 1 (FSTL1), also known as FSTL1 and follistatin-related protein (FRP), is a secreted protein that contains a follistatin-like domain, a Kazal-like domain and a von Willebrand factor C domain. Human FSTL1 shares 92% amino acid sequence identity with the mouse FSTL1, also known as TSC-36.
Follistatin-like 1/FSTL1 Antibody (228208)
R&D Systems | Catalog # MAB17381
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunocytochemistry
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 228208
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Follistatin-like 1/FSTL1
Glu19-Ile306
Accession # NP_032073
Glu19-Ile306
Accession # NP_032073
Specificity
Detects mouse Follistatin-like 1/FSTL1 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Follistatin-like 1/FSTL1 Antibody (228208)
Follistatin-like 1/FSTL1 in PC‑12 Rat Cell Line.
Follistatin-like 1/FSTL1 was detected in immersion fixed PC-12 rat adrenal pheochromocytoma cell line using Rat Anti-Mouse/Rat Follistatin-like 1/FSTL1 Mono-clonal Antibody (Catalog # MAB17381) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Follistatin-like 1/FSTL1 Antibody (228208)
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed PC-12 rat adrenal pheochromocytoma cell line
Sample: Immersion fixed PC-12 rat adrenal pheochromocytoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Follistatin-like 1/FSTL1
Alternate Names
Flik, Follistatin-related Protein, FRP, FSL1, FSTL1, TSC-36
Gene Symbol
FSTL1
UniProt
Additional Follistatin-like 1/FSTL1 Products
Product Documents for Follistatin-like 1/FSTL1 Antibody (228208)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Follistatin-like 1/FSTL1 Antibody (228208)
For research use only
Related Research Areas
Citations for Follistatin-like 1/FSTL1 Antibody (228208)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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