< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.
The Quantikine Mouse/Rat RGM-A Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat RGM-A in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse RGM-A and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural RGM-A showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural RGM-A.
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
The recovery of mouse/rat RGM-A spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Mouse Cell Culture Supernates (n=4)
Mouse EDTA Plasma (n=4)
Mouse Heparin Plasma (n=4)
Mouse Serum (n=4)
Mouse Tissue Homogenates (n=4)
Rat Cell Culture Supernates (n=4)
Rat EDTA Plasma (n=4)
Rat Heparin Plasma (n=4)
Rat Serum (n=4)
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RGM-A were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
The repulsive guidance molecule (RGM) family of molecules contains three uniquely structured GPI-linked glycoproteins that participate in a variety of activities. Termed RGM-A, -B and -C, they are 40-45 kDa in size, show generally nonoverlapping fetal expression patterns, and serve as BMP-4 receptors. RGM-A and -C are plasma membrane bound, while RGM-B is generally microsomal in location. RGM-A and -B are associated with neurons, while RGM-C regulates skeletal muscle and hepatocyte function.
Repulsive Guidance Molecule A
Entrez Gene IDs
56963 (Human); 244058 (Mouse);
RGM domain family member A; RGM domain family, member A; RGMA; RGM-A; RGMrepulsive guidance molecule A;
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