Recombinant and natural mouse and rat RGM-C/Hemojuvelin
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference observed with 1 or more available related molecules.
The Quantikine Mouse/Rat RGM-C/Hemojuvelin Immunoassay is a 4.5 hour solid phase
ELISA designed to measure mouse and rat RGM-C in cell culture
supernates, tissue homogenates, serum, and plasma. It contains
NS0-expressed recombinant mouse RGM-C and antibodies raised against the
recombinant factor. This immunoassay has been shown to quantitate the
recombinant factor accurately. Results obtained using natural RGM-C
showed dose-response curves that were parallel to the standard curves
obtained using the recombinant kit standards. These results indicate
that this kit can be used to determine relative mass values for natural
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays
to determine inter-assay precision. Assays were performed by at least
three technicians using two lots of kit components.
The recovery of mouse/rat RGM-C spiked to levels throughout the range of the assay in various matrices was evaluated.
Average % Recovery
Cell Culture Supernates (n=4)
EDTA Plasma (n=4)
Heparin Plasma (n=4)
To assess the linearity of the assay, samples containing high concentrations of RGM-C were serially diluted with Calibrator
Diluent to produce samples with values within the dynamic range of the
Preparation and Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
The repulsive guidance molecule (RGM) family of molecules contains three uniquely structured GPI-linked glycoproteins that participate in a variety of activities. Termed RGM-A, -B and -C, they are 40-45 kDa in size, show generally nonoverlapping fetal expression patterns, and serve as BMP-4 receptors. RGM-A and -C are plasma membrane bound, while RGM-B is generally microsomal in location. RGM-A and -B are associated with neurons, while RGM-C regulates skeletal muscle and hepatocyte function.
Repulsive Guidance Molecule C
Entrez Gene IDs
148738 (Human); 69585 (Mouse); 310681 (Rat);
DL-M; haemojuvelin; hemochromatosis type 2 (juvenile); Hemojuvelin; HFE2; HFE2AMGC23953; HJV; HJVHemochromatosis type 2 protein; JH; repulsive guidance molecule c; RGMC; RGM-C; RGMCRGM domain family member C;
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