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Mouse/Rat SIGNR3/CD209d Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB7220U Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB7220U-100UG

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Mouse/Rat SIGNR3/CD209d Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat SIGNR3/CD209d in Western blots. In Western blots, 100% cross-reactivity with recombinant mouse (rm) SIGNR1, 50% cross-reactivity with rmSIGNR7, and no cross-reactivity with rmSIGNR4 is observed. In direct ELISAs, no cross-reactivity
Source
Monoclonal Rat IgG2a Clone # 728804
Purification
Protein A or G purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SIGNR3/CD209d
Glu82-Lys237
Accession # Q91ZW8
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SIGNR3/CD209d

SIGNR3 (designated CD209d) is a 237 amino acid (aa), ~34 kDa type II transmembrane glycoprotein that is one of several mouse orthologs of human DC-SIGN (CD209). SIGNR3 is a calcium-binding C-type lectin that is expressed on myeloid mononuclear cells and a subpopulation of dendritic cells in the dermis, blood, and most lymphoid organs. Like human DC-SIGN, SIGNR3 recognizes high-mannose and fucose-terminated glycans and protects against pathogens such as Candida albicans and Mycobacterium tuberculosis. Within the region used as an immunogen, mouse and rat SIGNR3 share 91% aa sequence identity. A 207 aa isoform lacks aa 45-74, which includes the transmembrane domain.

Long Name
DC-SIGN-related Protein 3
Entrez Gene IDs
170779 (Mouse); 363856 (Rat)
Alternate Names
CD209 antigen;CDSIGN;CLEC4L;DC-SIGN;DC-SIGN1;hDC-SIGN; CD209d; SIGNR3

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